CD8A Recombinant Monoclonal Antibody

說明書

中文名稱：

CD8A重組抗體
貨號：

CSB-RA934881A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: CD8A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26, 22, 31 kDa
Observed band size: 31 kDa

IHC image of CSB-RA934881A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA934881A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunoprecipitating CD8A in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA934881A0HU in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2:CSB-RA934881A0HU(3μg)+ HepG2 whole cell lysate(500μg)
Lane 3: HepG2 whole cell lysate (10μg)
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA934881A0HU CD8A重組單克隆抗體是針對人源CD8α鏈開發(fā)的高特異性科研工具，適用于多種實(shí)驗(yàn)場景。該抗體識別的CD8A抗原是細(xì)胞毒性T淋巴細(xì)胞表面關(guān)鍵的共受體分子，通過與MHC I類分子相互作用參與抗原識別及T細(xì)胞活化，在適應(yīng)性免疫應(yīng)答中發(fā)揮重要作用。經(jīng)嚴(yán)格驗(yàn)證，本品在蛋白免疫印跡中能清晰檢測CD8A特異性條帶，免疫組化可精準(zhǔn)定位組織切片中CD8+ T細(xì)胞分布，免疫沉淀實(shí)驗(yàn)有效富集目標(biāo)蛋白，并在ELISA體系中展現(xiàn)優(yōu)異結(jié)合性能，推薦使用濃度分別為WB 1:500-1:5000、IHC 1:50-1:200、IP 1:200-1:1000。本產(chǎn)品采用重組表達(dá)技術(shù)保證批次穩(wěn)定性，經(jīng)多實(shí)驗(yàn)平臺驗(yàn)證顯示低交叉反應(yīng)性，適用于T細(xì)胞亞群分析、免疫微環(huán)境研究、感染性疾病模型構(gòu)建及腫瘤免疫相關(guān)機(jī)制探索等科研領(lǐng)域，為免疫細(xì)胞表型鑒定和功能研究提供可靠解決方案。
Uniprot No.：

P01732
基因名：

CD8A
別名：

T-cell surface glycoprotein CD8 alpha chain (T-lymphocyte differentiation antigen T8/Leu-2) (CD antigen CD8a), CD8A, MAL
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human CD8
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

2F2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IP	1:200-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
基因功能參考文獻(xiàn)：
1. Studied lymphocyte phenotype in resected lymph nodes of patients with lung cancer by analyzing levels of Foxp3 and CD8 by immunohistochemical staining. PMID: 28831395
2. This study compares the differences of PD-L1 expression and CD8+ tumor-infiltrating lymphocyte density, two major response biomarkers of PD-1/PD-L1 blockade, between paired primary and brain metastatic lesions in advanced non-small cell lung cancer (NSCLC). For NSCLC brain metastases patients, CD8+ tumor-infiltrating lymphocyte density showed a spatial and temporal heterogeneity. PMID: 29526752
3. Endometrioid endometrial carcinomas are capable of down-regulating CD8 expression by cytotoxic T lymphocytes. PMID: 27346574
4. The PD-1/CD8 ratio may, therefore, be a useful prognostic marker for stage II/III CRC. What is important for predicting the prognosis may be the PD-1/CD8 ratio rather than the absolute number of PD-1(+) tumor-infiltrating lymphocytes . PMID: 28739701
5. We also observed a significant association between forkhead box protein 3 ( p = 0.001) and the Neo-Bioscore, while only a marginal difference was observed with CD8+ expression ( p = 0.074). This study demonstrated that forkhead box protein 3 expression has value as the only independent marker that predicts a good response to neoadjuvant chemotherapy and that it is related with a good prognosis according to the Neo-Biosc PMID: 28621227
6. missense mutation increases susceptibility to recurrent respiratory infections PMID: 26563160
7. Human mesenchymal stromal cells enhance the immunomodulatory function of CD8(+)CD28(-) regulatory T cells. PMID: 25482073
8. Dendritic cells accumulate in the bone marrow of multiple myeloma patients and protect tumor plasma cells from CD8+ T-cell killing. PMID: 26185130
9. Patients with the presence of CD8- and CD45RO-positive T cells in bone marrow demonstrated better survival of gastric cancer patients than those with the absence of these cells in bone marrow. PMID: 25804232
10. More sCD8 circulating molecules were found in patients transfused with post-storage leucodepleted RBCs whose supernatants had large amounts of sHLA-I. sCD8 is a possible modulator of sHLA-I-mediated transfusion-related immunomodulation. PMID: 23356971
11. CD8-positivity proved to be an independent prognostic predictor of worse outcome in pediatric anaplastic lymphoma kinase-positive anaplastic large cell lymphoma. PMID: 23716548
12. CREMalpha orchestrates epigenetic remodeling of the CD8A,B through the recruitment of DNA methyltransferase (DNMT) 3a and histone methyltransferase G9a. PMID: 24297179
13. Modified CD8A gene function may contribute to the development of refractory chronic rhinosinusitis via reduction of circulating CD8 lymphocytes. PMID: 23640800
14. transcription factor cAMP-responsive element modulator alpha (CREMalpha), which is expressed at increased levels in T cells from systemic lupus erythematosus patients, contributes to transcriptional silencing of CD8A and CD8B. PMID: 24047902
15. The CD8(+)CCR7(+) T-cell frequency in HNSCC patients' blood tested at diagnosis can discriminate them from normal controls and predicts disease recurrence PMID: 23363813
16. The expression of human leucocyte antigen (HLA)-DR in epithelial cells and cluster of differentiation (CD8)-positive lymphocytes as possible markers of chronic ocular graft versus host disease, was investigated. PMID: 23878500
17. HIV-1 clade-B Nef peptides elicit increased frequency, breadth and function of CD8+ T-cells compared to consensus peptides PMID: 23185362
18. analysis of the effector phenotype of CD8+ T cells at the junction epithelium during clinical quiescence of herpes simplex virus 2 infection PMID: 22811543
19. study confirms that CD8 expression is a recurrent albeit rare phenomenon in patients with chronic lymphocytic leukemia and suggests that CD8 expression has an adverse prognostic impact PMID: 22143984
20. Silencing Hsp25/Hsp27 gene expression augments proteasome activity and increases CD8+ T-cell-mediated tumor killing and memory responses PMID: 22185976
21. LNX1 and LNX2 interact with CD8alpha and promote its ubiquitylation and endocytosis. PMID: 22045731
22. Nef-mediated down-modulation of CD8alphabeta is a fundamental property of primate lentiviruses. PMID: 22013062
23. Multiple sclerosis is characterized by a dysregulation of CD8+CD56-perforin+ T cells that may play a role in the development of disability. PMID: 22001684
24. Lymphoid tissue (LT)-resident CD8alpha-positive dendritic cells (DCs) and non(LT)-derived CD103-positive DCs express transgenic XCR1 and are characterized by a unique transcriptional fingerprint, irrespective of their tissue of origin. PMID: 21948982
25. Coreceptor CD8alphabeta contributes to the antigen-recognition process by binding to a largely invariant region of the MHC class I molecule and by promoting intracellular signaling. PMID: 21954283
26. These results suggest that the elevated frequency of PD-1(+) CD8(+) T cells could be associated with leukemic relapse in pediatric umbilical cord blood transplantation recipients. PMID: 21813446
27. The aim was to determine if SPARC, FOXP3, CD8 and CD45RO expression levels are associated with colorectal cancer (CRC) stage, disease outcome and long-term cancer-specific survival PMID: 21818290
28. we present the complex structures of human CD8alphaalpha bound to HLA-A*2402 PMID: 21645925
29. the presence of IL-1 and/or IL-6 during activation of human CD8 T cells attenuates Fas-mediated AICD, whereas IL-12 increases the susceptibility of activated CD8 T cells to this form of cell death PMID: 21518761
30. In the setting of HIV-induced lymphopenia, naive CD4-expressing T cells are recruited mainly into the proliferating pool in response to CD4 T cell depletion, whereas naive CD8-positive T cell proliferation is driven mainly by levels of HIV RNA. PMID: 21257970
31. Studies indicate that The thymus leukemia (TL) antigen and CD8alphaalpha are interacting surface molecules that are expressed at the frontline of the mucosal immune system. PMID: 20850477
32. PD-L1 and PD-L2 knockdown dendritic cells showed superior potential to expand minor histocompatibility antigen-specific CD8(+) effector and memory T cells from leukemia patients early after donor lymphocyte infusion and later during relapse. PMID: 20682852
33. The study shows that impaired Epstein-Barr virus-specific CD8+ T-cell function in X-linked lymphoproliferative disease is restricted to SLAM family-positive B-cell targets. PMID: 20644117
34. The positive rate of CD8 in primary lung cancer tissue was significantly higher than that in normal lung tissue. PMID: 20704820
35. Binding of the CD8 coreceptor to class I histocompatibility molecules significantly enhances the amount of calcium released from intracellular stores, triggered by a weaker ESO1 peptide agonist. PMID: 20053942
36. Four new weak but tissue-specific DNase I hypersensitivity sites have been mapped 0-0.8 kb upstream of the first exon of CD8A, in the promoter region. PMID: 11937547
37. Soluble HLA-A,-B,-C and -G molecules induce apoptosis in T and NK CD8+ cells and inhibit cytotoxic T cell activity through CD8 ligation. PMID: 12594841
38. expression of the HMG box protein TOX is sufficient to induce changes in coreceptor gene expression associated with beta-selection, including CD8 gene demethylation PMID: 15078895
39. Establishment of a permanent CD8+ cell line from a patient with leukemic phase of acute lymphoblastic lymphoma. PMID: 15927673
40. p32 recruits ganciclovir kinase to redistribute the nuclear lamina. PMID: 15975922
41. Introduction of CD8 alpha down-regulates the production of major T helper (Th) type 2 cytokines and may induce differentiation of tumor-specific Th1 cells to improve the clinical potential of T cell receptor (TCR) gene transfer to treat melanoma. PMID: 16818755
42. The results indicate that p.Gly111Ser is confined to the Spanish Gypsy population, where it occurs at a carrier rate of 0.4%. PMID: 17658607
43. human monocytes express CD8 alpha; co-engagement of CD8 alpha and FcR enhances monocyte TNF release, suggesting FcR may be a novel partner receptor for CD8 alpha on innate immune cells PMID: 17678538
44. CD8+FOXP3+ T cells play an important role in unresponsiveness related to upregulated interleukin receptors on dendritic cells. PMID: 18089323
45. Data suggest that Bim-mediated attrition of HBV-specific CD8(+) T cells contributes to the inability of these cell populations to persist and control viral replication. PMID: 18398508
46. tumor-induced TGF-beta may actively subvert the CD8+ arm of the immune system into directly promoting tumor growth by an IL-17-dependent mechanism PMID: 18483277
47. Data suggest that a decrease in CD8+CD28- T cells may reflect impaired T-cell suppression and accordingly, increased T cell help to autoreactive B cells in patients with systemic lupus erythematosus. PMID: 18625635
48. analysis of an interaction between B7-H3 and TLT-2 that preferentially enhances CD8(+) T cell activation PMID: 18650384
49. polarized CD8 T cells within the HBV-infected liver may impede proliferative antiviral effector function, while contributing to the proinflammatory cytokine environment PMID: 18695005
50. in patients with melanoma, who underwent a peptide-based vaccination, homodimer may represent a marker of antigen-specific T-cell memory PMID: 18833003
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相關(guān)疾病：

CD8 deficiency, familial (CD8 deficiency)
亞細(xì)胞定位：

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
組織特異性：

CD8 on thymus-derived T-cells usually consists of a disulfide-linked alpha/CD8A and a beta/CD8B chain. Less frequently, CD8 can be expressed as a CD8A homodimer. A subset of natural killer cells, memory T-cells, intraepithelial lymphocytes, monocytes and
數(shù)據(jù)庫鏈接：

HGNC: 1706

OMIM: 186910

KEGG: hsa:925

STRING: 9606.ENSP00000283635

UniGene: Hs.85258

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