WDR1 Recombinant Monoclonal Antibody

說明書

中文名稱：

WDR1 Recombinant Monoclonal Antibody
貨號(hào)：

CSB-RA094686A0HU
規(guī)格：

￥1320
圖片：
Flow cytometric analysis of WD repeat domain 1 expression in H9C2 cells using WD repeat domain 1 antibody. Green, isotype control; red, WD repeat domain 1.

Immunocytochemical staining of H9C2 cells with WD repeat domain 1 antibody. Nuclei were stained blue with DAPI; WD repeat domain 1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using WD repeat domain 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WD repeat domain 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

Uniprot No.：

O75083
基因名：

WDR1
別名：

WDR1; WD Repeat Domain 1; AIP1; Actin-Interacting Protein 1; WD Repeat-Containing Protein 1; NORI-1; Epididymis Secretory Protein Li 52; HEL-S-52; PFITS
反應(yīng)種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human WDR1 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

21E11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. Enhances cofilin-mediated actin severing. Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions. Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance. Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension. Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt.
基因功能參考文獻(xiàn)：
1. STAT3 bound to the 5' upstream sequence (-1971 to -1964), a putative promoter region, of WDR1 gene, and its activation induced WDR1 overexpression in breast cancer cells. PMID: 27521604
2. MRTF-A-miR-206-WDR1 form feedback loop to regulate breast cancer cell migration. PMID: 28822708
3. homozygous missense L153F/L293F mutation in the actin regulatory gene WDR1 causes a new autoinflammatory disease in humans, with periodic fevers, immunodeficiency, and intermittent thrombocytopenia (PFIT). PMID: 27994071
4. These data suggest that WDR1 plays an important role in suppressing platelet activity, where it alters the actin cytoskeleton dynamics, and downregulation of WDR1 may contribute to the platelet-mediated pathogenesis of cardiovascular disease. PMID: 27609643
5. Mutations in WDR1 affect neutrophil morphology, motility, and function, causing a novel primary immunodeficiency PMID: 27557945
6. A/A" genotype of rs12498927 was associated with increased risk of gout. PMID: 27173277
7. Identify positive correlations between WDR1 and CLNK gene polymorphisms in Chinese-Tibetan gout populations. PMID: 26438387
8. Demonstrate a previously unanticipated role of Aip1 in regulating the structure and remodeling of intestinal epithelial junctions and early steps of epithelial morphogenesis. PMID: 25792565
9. When maternal environmental tobacco smoke was considered, fifteen of 135 single nucleotide polymorphism (SNPs) mapping to SLC2A9 and 9 of 59 SNPs in WDR1 gave P values approaching genome-wide significance. PMID: 24516586
10. Data suggest that anti-WD repeat domain 1 (WDR1) antibody could be applied for serological screening of papillary thyroid carcinoma (PTC) and undifferentiated thyroid carcinoma (UTC), and could be an efficient and inexpensive biomarker. PMID: 23215816
11. WD repeat-containing protein 1 was confirmed as being significantly overexpressed in interstitial fluid from ovarian carcinomas compared to healthy ovarian tissue. PMID: 23707566
12. Studies suggest that vascular endothelial growth factor may be responsible for upregulated P-glycoprotein expression. PMID: 23293323
13. The QKI RNA-binding proteins regulate oligodendrocyte differentiation by modulating the expression of AIP-1. PMID: 20631256
14. Studies indicate that CUL4 uses a large beta-propeller protein, DDB1, as a linker to interact with a subset of WD40 proteins. PMID: 19818632
15. AIP1/WDR1 could abort the severing/disassembly activity somewhat still carried out due to phosphorylated ADF/cofilin PMID: 15629458
16. Data indicates that Aip1 plays an important role in directional cell migration by restricting the stimulus-induced membrane protrusion to one direction via promoting cofilin activity. PMID: 18494608
17. Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527
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亞細(xì)胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, podosome. Cell junction.
蛋白家族：

WD repeat AIP1 family
組織特異性：

Expressed in peripheral blood mononuclear cells (at protein level).
數(shù)據(jù)庫鏈接：

HGNC: 12754

OMIM: 604734

KEGG: hsa:9948

STRING: 9606.ENSP00000427687

UniGene: Hs.128548

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