USP9X Recombinant Monoclonal Antibody

說明書

中文名稱：

USP9X Recombinant Monoclonal Antibody
貨號：

CSB-RA183712A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA183712A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA183712A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing Hela cells stained with CSB-RA183712A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

Q93008
基因名：

USP9X
別名：

Probable ubiquitin carboxyl-terminal hydrolase FAF-X (EC 3.4.19.12) (Deubiquitinating enzyme FAF-X) (Fat facets in mammals) (hFAM) (Fat facets protein-related, X-linked) (Ubiquitin thioesterase FAF-X) (Ubiquitin-specific protease 9, X chromosome) (Ubiquitin-specific-processing protease FAF-X), USP9X, DFFRX FAM USP9
反應種屬：

Human
免疫原：

A synthesized peptide from human USP9X protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

11F2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-48'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Involved in axonal growth and neuronal cell migration. Regulates cellular clock function by enhancing the protein stability and transcriptional activity of the core circadian protein ARNTL/BMAL1 via its deubiquitinating activity.
基因功能參考文獻：
1. USP9X is overexpressed in breast carcinomas, and its level of expression is correlated with that of CEP131. USP9X is an important regulator of centrosome biogenesis and USP9X/CEP131 plays a critical role in breast carcinogenesis. PMID: 28361952
2. High USP9X expression is associated with Pancreatic Ductal Adenocarcinoma. PMID: 29248719
3. USP9X is a deubiquitylase of the Hippo pathway kinase LATS2 and that the Hippo pathway functions as a downstream signaling cascade that mediates USP9X's tumor-suppressive activity. PMID: 29183995
4. Ets-1 is induced by BRAF or MEK kinase inhibition, resulting in increased NRAS expression, which could be blocked by inactivation of Usp9x. PMID: 28198367
5. LNC473 could recruit deubiquitinase USP9X to inhibit the ubiquitination level of survivin and then increase survivin expression. PMID: 29605299
6. Study shows that USP9X deubiquitylating enzyme maintains RAPTOR protein levels, mTORC1 signalling and proliferation in neural progenitors. USP9X is the first deubiquitylating enzyme shown to stabilize RAPTOR. PMID: 28341829
7. Deubiquitylating enzyme USP9X was overexpressed in gastric cancer, suggesting a potential implication as an oncogene, and was significantly associated with a poorer survival. PMID: 28274596
8. Study demonstrated that USP9X acts as a tumor metastasis supporter in pancreatic ductal adenocarcinoma (PDAC), and its downregulation inhibited the migration and invasion of PDAC cells, while high expression of USP9X inhibited the apoptosis of cancer cells. Therefore, USP9X functioned as an oncogene in PDAC cells and is closely related with the expression of Snail, Twist and survivin. PMID: 29130109
9. knockdown of USP9X was shown to confer resistance to apoptosis following pediatric T-cell acute lymphoblastic leukemia relevant chemotherapy drug treatment in Jurkat leukemia cells PMID: 27602765
10. USP9X stabilizes beta-catenin and activates Wnt/beta-catenin signal pathway to promote glioma cell proliferation and survival. PMID: 27783990
11. Loss of USP9X expression is associated with pancreatic cancer. PMID: 28720576
12. Frame shift mutation in USP9X and deletion of the 5'UTR of the USP9X identified in two females with intellectual disability syndrome. PMID: 28377321
13. High USP9X expression is associated with basal-like breast cancer. PMID: 27593927
14. The authors find that primary human aggressive B-cell lymphoma samples exhibit high USP9X expression that correlate with XIAP overexpression. PMID: 27317434
15. USP9x-SMAD4 Interaction is associated with Breast Cancer Metastasis. PMID: 28115363
16. Data suggest that USP9X as an integral component of centrosome where it functions to stabilize PCM1 and CEP55 and to promote centrosome biogenesis; N-terminal domain of USP9X appears to be responsible for physical association of USP9X with PCM1 and CEP55. (USP9X = ubiquitin-specific protease 9X; PCM1 = pericentriolar material 1 protein; CEP55 = 55kDa centrosomal protein) PMID: 28620049
17. USP9X recruited to the centrosome by NPHP5 protects NPHP5 from ubiquitination, thus favouring cilia assembly. PMID: 28498859
18. BAG3 was found to positively regulate Mcl-1 levels by binding to and inhibiting USP9X. Our data show that BAG3 and Mcl-1 are key mediators of resistance to chemotherapy in ovarian cancer PMID: 27120977
19. found that USP9X regulated the expression and stability of CLASPIN in an S-phase-specific manner. USP9X depletion profoundly impairs the progression of DNA replication forks, causing unscheduled termination events with a frequency similar to CLASPIN depletion, resulting in excessive endogenous DNA damage PMID: 26921344
20. USP9X possibly promotes head and neck cancer cell proliferation through the mTOR pathway. PMID: 27374971
21. biological relevance of the ICP0- USP9X complex in HSV-1 infection PMID: 26596467
22. identify USP9X as a novel regulator of EGFR endocytosis PMID: 26748853
23. study reports for the first time that USP9X is a deubiquitinase of Angiomotin-like 2 (AMOTL2) and that AMOTL2 mono-ubiquitination is required for YAP inhibition PMID: 26598551
24. USP9x interacted with and stabilized beta-catenin through deubiquitination to mediate transcription of the decoy receptors in breast cancer cells PMID: 26717875
25. We examined the role of USP9X in the primary cilium of affected females with ciliopathy syndromes and found that endogenous USP9X localizes along the length of the ciliary axoneme, so its loss of function could disrupt cilium-regulated processes. PMID: 26833328
26. These data support the use of MCL1 expression as a predictive biomarker for USP9X inhibitors in non-small cell lung cancer therapy PMID: 25692226
27. The mammalian PRICKLE-interactome was defined, identifying prickle-interacting proteins that localize to synapses and a novel interacting partner, USP9X, a substrate-specific de-ubiquitinase. PRICKLE and USP9X interact through their carboxy-termini; and USP9X de-ubiquitinates PRICKLE, protecting it from proteasomal degradation. PMID: 25763846
28. the novel compound EOAI3402143 dose-dependently inhibited Usp9x and Usp24 activity, increased tumor cell apoptosis, and fully blocked or regressed myeloma tumors in mice. PMID: 25814533
29. Noxa upregulation reduces the availability of Usp9x to Mcl-1, thereby promoting its ubiquitination and degradation, leading to the apoptosis of neoplastic cells. PMID: 24991768
30. Although USP9X may function as a tumor-suppressor during the establishment of PDAC, data presented here argue that USP9X promotes cell growth in advanced PDAC cells when PDAC is typically diagnosed. PMID: 24841553
31. Our analysis of clinical HCC samples verifies that miR-26b also targets USP9X expression to inhibit the EMT of hepatocytes PMID: 24890815
32. USP9X downregulation renders breast cancer cells resistant to tamoxifen. PMID: 25028367
33. USP9X mutations may have a role in X-linked intellectual disability and disrupt neuronal cell migration and growth PMID: 24607389
34. a large set of SOX2-associated proteins in DAOY medulloblastoma cells PMID: 23667531
35. Mcl-1, Bcl-xL and USP9X overexpression are tumor survival mechanisms protective against chemotherapy. PMID: 23171055
36. The resulting USP9X-deficient cancer cells exhibited increased activation of apoptotic pathways. PMID: 22895071
37. USP9X is an important regulatory protein of SMURF1. PMID: 23184937
38. Usp9x plays an important role in stabilizing SMN and the SMN complex, likely via antagonizing Ub-dependent SMN degradation. PMID: 23112048
39. the deubiquitinase USP9X as a novel mTORC1 and -2 binding partner that negatively regulates mTOR activity and skeletal muscle differentiation. PMID: 22544753
40. loss of Usp9x enhances transformation and protects pancreatic cancer cells from anoikis PMID: 22699621
41. Our results suggest that ubiquitin-specific protease 9X (USP9X) is by far the most active deubiquitinase acting on Ub-PEX5, both in female rat liver and HeLa cells. PMID: 22371489
42. alpha-Synuclein fate is determined by USP9X-regulated monoubiquitination PMID: 22065755
43. Zymophagy, a novel selective autophagy pathway mediated by VMP1-USP9x-p62, prevents pancreatic cell death. PMID: 21173155
44. Ubiquitin-cycle inhibition represents a novel and effective approach to blocking Bcr-Abl kinase signaling and reducing Mcl-1 levels to engage chronic myelogenous leukemia cell apoptosis. PMID: 21248063
45. the deubiquitinase USP9X stabilizes MCL1 and thereby promotes cell survival; deubiquitinases may stabilize labile oncoproteins in human malignancies PMID: 20023629
46. FAM associates with E-cadherin and beta-catenin during trafficking to the plasma membrane PMID: 14742711
47. identified the ubiquitin-protease FAM/USP9X as a binding partner of Itch. The association between Itch and FAM/USP9X was confirmed in vitro by glutathione S-transferase pulldown and in vivo through coimmunoprecipation PMID: 17038327
48. NUAK1 and MARK4 are substrates of USP9X PMID: 18254724
49. Smad4 is monoubiquitinated in lysine 519 in vivo, a modification that inhibits Smad4 by impeding association with phospho-Smad2; FAM reverts this negative modification, re-empowering Smad4 function. PMID: 19135894
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相關疾病：

Mental retardation, X-linked 99 (MRX99)
亞細胞定位：

Cytoplasm. Cell projection, growth cone.
蛋白家族：

Peptidase C19 family
組織特異性：

Widely expressed in embryonic and adult tissues.
數據庫鏈接：

HGNC: 12632

OMIM: 300072

KEGG: hsa:8239

STRING: 9606.ENSP00000316357

UniGene: Hs.77578

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