TUBG1 Recombinant Monoclonal Antibody
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中文名稱：TUBG1 Recombinant Monoclonal Antibody
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貨號(hào)：CSB-RA936218A0HU
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規(guī)格：￥1320
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圖片：
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Flow cytometric analysis of gamma Tubulin expression in HepG2 cells using gamma Tubulin antibody. Green, isotype control; red, gamma Tubulin. -
Immunocytochemical staining of HepG2 cells with Gamma Tubulin antibody. Nuclei were stained blue with DAPI; Gamma Tubulin was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. -
Western blotting analysis using gamma Tubulin antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with gamma Tubulin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：TUBG1
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別名：TUBG1; Tubulin Gamma 1; TUBGCP1; TUBG; Gamma-Tubulin Complex Component 1; Tubulin, Gamma Polypeptide; Tubulin Gamma-1 Chain; GCP-1; Tubulin, Gamma 1; Gamma-1-Tubulin; CDCBM4
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反應(yīng)種屬：Human, Mouse, Rat
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免疫原：Recombinant Human TUBG1 protein
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：21H12
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, FC, ICC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 FC 1:200-1:2000 ICC 1:100-1:1000 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
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基因功能參考文獻(xiàn)：
- CCT actively promotes the formation of conformationally different aggregates of gamma-tubulin, a non-amyloidogenic CCT client protein, which are mediated by specific CCT-gamma-tubulin interactions. PMID: 29339327
- that in the face of predominant gamma-tubulin-1 expression, the accumulation of gamma-tubulin-2 in mature neurons and neuroblastoma cells during oxidative stress may denote a prosurvival role of gamma-tubulin-2 in neurons PMID: 28119396
- All GCPs are incorporated into the helix via lateral interactions between their N-terminal domains, whereas the C-terminal domains mediate longitudinal interactions with gamma-tubulin. PMID: 27660388
- While binding to gamma-tubulin may help target APC to the site of microtubule nucleation complexes, additional C-terminal sequences of APC are required to stimulate and stabilize microtubule growth. PMID: 26556314
- Results show that CUL4A- and CUL4B-mediated polyubiquitination of gamma-tubulin for its degradation. PMID: 25542213
- In this study, the molecular basis of interaction between two lateral gamma-tubulin units within the gamma-tubulin ring complex were elucidated by making extensive use of molecular mechanics and molecular dynamics techniques. PMID: 25031076
- Gamma tubulin expression was associated with patients' age, astrocytoma grade, respectability, patient survival and performance. These factors may be important prognostic indicators for patients with astrocytomas. PMID: 24620973
- Recombinant gamma-tubulin can be phosphorylated by Cdk1-cyclin B or Brsk1 and dephosphorylated by Ppp4c-R2-R3A in vitro. PMID: 23966160
- Nek9 phosphorylates NEDD1 on Ser377 driving its recruitment and thereby that of gamma-tubulin to the centrosome in mitotic cells. PMID: 22818914
- Our results reveal for the first time an increased expression of TUBG1 and TUBG2 in lung cancer PMID: 22806905
- overexpression of Cdk1 or BRCA1 greatly expands the gamma-tubulin coating of microtubules, suggesting that the microtubule-bound gamma-tubulin is involved in DNA damage response. PMID: 21951856
- in transformed as well as in non-transformed interphase mammalian cells gamma-tubulin is present not only in cytoplasm but also in nuclei and nucleoli where it can be translocated during mitosis PMID: 21465471
- These results thus report for the first time the identification of Cep70 as an important centrosomal protein that interacts with gamma-tubulin and underscore its critical role in the regulation of mitotic spindle assembly. PMID: 21795687
- The data presented here indicate that gamma-tubulin has a profound relationship with actin filament dynamics PMID: 21473851
- a direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome PMID: 20224777
- the 2.7-A crystal structure of human gamma-tubulin bound to GTP-gammaS (a non-hydrolysable GTP analogue) PMID: 15917813
- analysis of human ninein isoforms that are regulated by centrosomal targeting signals and bind to gamma-tubulin PMID: 17102634
- Data suggest that aberrant expression and interactions of betaIII-tubulin and gamma-tubulin may be linked to malignant changes in glial cells. PMID: 17406983
- GSK-3beta regulates the localization of gamma-tubulin ring complex, including GCP5, to the spindle poles, thereby controlling the formation of proper mitotic spindles PMID: 18316369
- The axial elastic modulus of tubulin diminishes as the length of the monomer increases. PMID: 18621829
- Increased expression of centrosomal TUBG in atypical ductal hyperplasia and carcinoma of the breast is reported. PMID: 19215229
- Stability of the small gamma-tubulin complex (gamma tubulin/GCP2/GCP3) requires HCA66, a protein of the centrosome and the nucleolus. PMID: 19299467
- Data suggest that sequential phosphorylation of Nedd1 by Cdk1 and Plk1 plays a role in targeting gamma tubulin ring complex to the centrosome by promoting the interaction of Nedd1 with the gammaTuRC component gamma-tubulin, during mitosis. PMID: 19509060
- Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components PMID: 19543530
- SADB kinase activity controls centrosome homeostasis by regulating phosphorylation of gamma-tubulin. PMID: 19648910
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相關(guān)疾病：Cortical dysplasia, complex, with other brain malformations 4 (CDCBM4)
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亞細(xì)胞定位：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle.
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蛋白家族：Tubulin family
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