TSHR Recombinant Monoclonal Antibody

說明書

中文名稱：

TSHR Recombinant Monoclonal Antibody
貨號：

CSB-RA025131MA1HU
規格：

￥1320
圖片：
The Binding Activity of Human TSHR with Anti-TSHR Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TSHR (CSB-MP025131HU) at 10 μg/mL can bind Anti-TSHR recombinant antibody. The EC₅₀ is 30.81-36.12 ng/mL.The VLPs (CSB-MP3838) is negative control.

Untransfected HEK293T cells (green line) and transfected Human TSHR HEK293T stable cells (red line) were stained with anti-TSHR antibody (CSB-RA025131MA1HU) (2μg/1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

The purity of TSHR was greater than 95% as determined by SEC-HPLC
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA025131MA1HU重組單克隆抗體是針對甲狀腺刺激激素受體（TSHR）開發的高特異性科研工具。TSHR作為G蛋白偶聯受體家族成員，在調控甲狀腺激素合成與分泌中發揮核心作用，其異常活化與Graves病、甲狀腺功能亢進及甲狀腺腫等病理過程密切相關。本產品經ELISA平臺嚴格驗證，展現優異的抗原結合能力，在1:5000至1:50000的寬泛稀釋范圍內均能保持穩定的檢測靈敏度，適用于梯度濃度實驗設計。其重組單抗特性確保批次間高度一致性，為甲狀腺相關信號通路研究、受體功能解析及體外模型構建提供可靠支持。科研人員可將其應用于甲狀腺細胞系中TSHR蛋白表達水平的定量分析、受體配體相互作用研究，以及甲狀腺疾病機制探索中的靶點驗證實驗，為藥物靶點篩選和分子機制研究提供精準檢測方案。
Uniprot No.：

P16473
基因名：

TSHR
別名：

CHNG1 antibody; hTSHR I antibody; hTSHRI antibody; LGR 3 antibody; LGR3 antibody; MGC75129 antibody; Seven transmembrane helix receptor antibody; Thyroid adenoma hyperfunctioning antibody; Thyroid carcinoma with thyrotoxicosis antibody; Thyroid stimulating hormone receptor antibody; Thyroid stimulating hormone receptor; isoform 2 antibody; Thyroid-stimulating hormone receptor antibody; Thyrotropin receptor antibody; Thyrotropin receptor I antibody; Thyrotropin receptor I; hTSHR I antibody; TSH R antibody; TSH Receptor antibody; TSH-R antibody; TSHR antibody; TSHR_HUMAN antibody
反應種屬：

Human
免疫原：

Recombinant Human TSHR protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號：

11A5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:5000-1:50000

Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism.
基因功能參考文獻：
1. MCT8 and TSHR form heteromers. PMID: 29290039
2. TSHR mutations occur in approximately 5% thyroid nodules in a large consecutive series with indeterminate cytology. TSHR mutations may be associated with an increased cancer risk when present at high allelic frequency PMID: 29516685
3. This work is devoted to the ascertainment of serological cross-reactivity between OmpF porin from Yersinia pseudotuberculosis (YpOmpF) and human thyroid-stimulating hormone receptor (hTSHR). PMID: 29079441
4. Genetic polymorphisms of CTLA-4 gene on the nucleotide 49 at codon 17 of exon 1, TSHR gene SNP rs2268458 of intron 1, number of regulatory T cells and TRAb levels play a role as risk factors for relapse in patients with Graves' disease. PMID: 29093229
5. Overexpression of TSHR was found in a great majority of hepatocellular carcinomatissues and associated with unfavorable prognosis PMID: 29715101
6. Signaling dissection using diverse inhibitors indicated that EOC cell proliferation driven by thyrostimulin-TSHR signaling is PKA independent, but does require the involvement of the MEK-ERK and PI3K-AKT signal cascades, which are activated mainly via the trans-activation of EGFR PMID: 27273257
7. SNPs rs179247 and rs12101255 were significantly associated with Graves disease. rs12101255 and rs2268458 polymorphisms had no association with Graves diseases and Graves ophthalmopathy. PMID: 27456991
8. Among the evaluated TSHR gene SNPs, the rs4411444 GG genotype and the rs4903961 C allele in the enhancer regions of the TSHR gene were most strongly associated with the development of Graves disease, especially intractable disease, and that of Hashimoto disease, respectively. PMID: 27762730
9. Low expression of TSHR is associated with dilated cardiomyopathy and impaired left ventricular function accompanied by increased risk of death. PMID: 29320567
10. Monoallelic TSHR mutations are significantly associated with positive newborn screen for congenital hypothyroidism, and the association is further strengthened by the coexistence of monoallelic DUOX2 mutations. PMID: 29092890
11. The role of TSH Receptor cleavage into subunits and shedding of the A-Subunit in Graves' disease pathogenesis is reviewed. PMID: 27454362
12. a hot-spot mutation in EZH1 is the second most frequent genetic alteration in autonomous thyroid adenomas; the association between EZH1 and TSHR mutations suggests a 2-hit model for the pathogenesis of these tumors, whereby constitutive activation of the cAMP pathway and EZH1 mutations cooperate to induce the hyperproliferation of thyroid cells PMID: 27500488
13. Germline mutation in the thyrotropin receptor gene is associated with non-autoimmune hyperthyroidism. PMID: 28195550
14. Congenital Hypothyroidism With Gland-in-Situ 59% attributable to mutations in TSHR. PMID: 27525530
15. data support the association that low intrathymic TSHR expression is associated with susceptibility to developing pathogenic TSHR antibodies, while high intrathymic TSHR expression is protective PMID: 28099999
16. Graves' disease is associated with polymorphisms of TSHR intron 1 rs179247 and rs12101255. There is no association between rs179247 SNPs and Graves' ophthalmopathy PMID: 27465319
17. TSHR gene mutations p.R528C and c.392+4del4 associated with congenital hypothyroidism. PMID: 26864598
18. this study shows that the TSHR is not main factor contributing to determining common genetic basis among Graves disease and alopecia areata PMID: 27810496
19. TSHR D727E polymorphism might be involved in the pathogenesis of toxic multinodular goiter PMID: 27525921
20. lncRNA PVT1 may contribute to tumorigenesis of thyroid cancer through recruiting EZH2 and regulating TSHR expression. PMID: 26427660
21. experimentally verified contact of Ser-281 (ECD) and Ile-486 (TMD) was subsequently utilized in docking homology models of the ECD and the TMD to create a full-length model of a glycoprotein hormone receptor PMID: 27129207
22. TSHR gene polymorphisms are associated with typical symptoms in primary congenital hypothyroidism. PMID: 26356361
23. The eminent ratio of TSHr methylation in well-differentiated thyroid carcinoma against benign thyroidal nodules adduced that TSHr methylation status can be utilized as a tumor marker for well-differentiated thyroid cancer. PMID: 26519197
24. Thyroid-stimulating hormone receptor intronic polymorphisms are associated with the susceptibility to Graves' disease and Graves' ophthalmopathy in the Brazilian population, but do not appear to influence the disease course. PMID: 25543543
25. data support a structural model of the TSHR transmembrane domain with a bulged transmembrane helix TM2 and a straight TM5 that is specific of glycoprotein hormone receptors PMID: 26545118
26. TSHR gene variants are associated with congenital hypothyroidism. PMID: 25153578
27. the hinge region and its adjacent domains have roles in binding and signaling patterns of the thyrotropin and follitropin receptor PMID: 25340405
28. subclinical hypothyroidism in heterozygotes with TSHR mutations is a stable compensated condition with an appropriately adjusted set point for pituitary-thyroid feedback that does not require replacement therapy PMID: 25557138
29. Patients with preoperative TSHR-mRNA >/=1.02 ng/mug may be at a greater risk for recurrence of thyroid cancer. PMID: 26212344
30. The present study demonstrates that the SmartAmp2 method is useful to detect the R450H mutation in TSHR. PMID: 24895636
31. The aim of this study was to investigate whether chimeric TSH receptor based bioassay can predict Graves' disease remission/relapse after antithyroid drug PMID: 24968734
32. TSHR polymorphisms were not associated with the dose of T4 or central obesity among hypothyroid patients. PMID: 25079464
33. TSHR mutations are common among Hungarian patients with Congenital hypothyroidism. The novel genetic alterations revealed an important structural role of the N432(1.50) and the P449(2.39) residues in receptor expression and signaling, respectively. PMID: 25978107
34. c.317+1G>A splice site mutation in the TSHR gene leads to sever congenital hypothyroidism PMID: 24859513
35. Our data suggest a role for PTCSC2, FOXE1, and TSHR in the predisposition to papillary thyroid carcinoma. PMID: 25303483
36. germline polymorphisms of TSHR do not confer susceptibility for the development of autonomously functioning thyroid nodules. PMID: 24789540
37. our study showed a high implication of TSHR gene methylation and its significant association with BRAF V600E mutation in thyroid tumors, depicting a positive connection between TSHR pathway and MAP Kinase pathway. PMID: 24927793
38. Allele A of the rs179247 polymorphism in the TSHR gene is associated with lower risk of GO in young GD patients. PMID: 25061884
39. study reports a large deletion of the TSHR gene in two siblings with congenital hypothyroidism coming from a consanguineous Turkish family PMID: 24690939
40. A gain-of-function mutation (M435R) activates the Gs/adenylyl cyclase pathway, deactivates the Gq/11-phospholipase C pathway, and causes nonautoimmune hyperthyroidism in a Japanese family. PMID: 24608569
41. This review covers several mutations in TSHR which are clearly associated with a hyperthyroidism-phenotype, but interestingly show a lack of constitutive activity determined by in vitro characterization. PMID: 24845969
42. A nonsense thyrotropin receptor gene mutation is associated with congenital hypothyroidism and heart defects. PMID: 24945425
43. A newly discovered TSHR mutation L665F in transmembrane helix 7 of the receptor was detected in six members of a family with hyperthyroidism. PMID: 24947036
44. findings demonstrate TSH-R expression is thymus-specific within the immune system; data support the notion of a novel neuroendocrine-immune interaction in which TSH-R signaling in the thymus, most likely mediated by TSH, enhances thymic T-cell development PMID: 24635198
45. Autonomous adenomas are caused by somatic mutations of the thyroid-stimulating hormone receptor in children. PMID: 24480816
46. genetic association studies in population in Japan: Data suggest that patients with TSHR mutations exhibit persistent hyperthyroidism throughout follow-up; in such patients, hyperthyroidism progresses despite treatment with antithyroid drugs. PMID: 24279482
47. Significant reduction in TSHR messenger RNA is associated with ovarian carcinomas. PMID: 24844218
48. discovered a genetic-epigenetic interaction involving a noncoding SNP in the TSHR gene that regulates thymic TSHR gene expression and facilitates escape of TSHR-reactive T cells from central tolerance, triggering Graves disease PMID: 25122677
49. The expression of TSHR and NIS genes is differently controlled by multiple mechanisms, including epigenetic events elicited by major signaling pathways involved in thyroid tumorigenesis. PMID: 24353283
50. Only the IL1RN tandem repeats polymorphism may be associated with Hashimoto's thyroiditis susceptibility; TSHR and IL1RN polymorphisms may represent prognostic factors for predicting the severity of the disease. PMID: 24328419
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相關疾病：

Hypothyroidism, congenital, non-goitrous, 1 (CHNG1); Familial gestational hyperthyroidism (HTFG); Hyperthyroidism, non-autoimmune (HTNA)
亞細胞定位：

Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family, FSH/LSH/TSH subfamily
組織特異性：

Expressed in thyroide cells (at protein level). Expressed in the thyroid.
數據庫鏈接：

HGNC: 12373

OMIM: 275200

KEGG: hsa:7253

STRING: 9606.ENSP00000298171

UniGene: Hs.160411

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