SUN1 Recombinant Monoclonal Antibody

說明書

中文名稱：

SUN1 Recombinant Monoclonal Antibody
貨號：

CSB-RA996476A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of SUN1 expression in HepG2 cells using SUN1 antibody. Green, isotype control; red, SUN1.

Immunocytochemical staining of H9C2 cells with SUN1 antibody. Nuclei were stained blue with DAPI; SUN1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using SUN1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SUN1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

O94901
基因名：

SUN1
別名：

SUN1; Sad1 And UNC84 Domain Containing 1; KIAA0810; UNC84A; SUN Domain-Containing Protein 1; Sad1 Unc-84 Domain Protein 1; Sad1/Unc-84 Protein-Like 1; Protein Unc-84 Homolog A; FLJ12407; Unc-84 Homolog A (C. Elegans); Unc-84 Homolog A
反應種屬：

Human
免疫原：

Recombinant Human SUN1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

20C9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Involved in telomere attachment to nuclear envelope in the prophase of meiosis implicating a SUN1/2:KASH5 LINC complex in which SUN1 and SUN2 seem to act at least partial redundantly. Required for gametogenesis and involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs). Required for efficient localization of SYNE4 in the nuclear envelope. May be involved in nuclear remodeling during sperm head formation in spermatogenenis. May play a role in DNA repair by suppressing non-homologous end joining repair to facilitate the repair of DNA cross-links.
基因功能參考文獻：
1. While Sun1 and Sun2 in HeLa cells are each able to bind KASH-domains, Sun1 is more efficiently incorporated into LINC complexes under normal growth conditions. Furthermore, the balance of Sun1 and Sun2 incorporated into LINC complexes is cell type-specific and is correlated with SRF/Mkl1-dependent gene expression. PMID: 29813079
2. Low SUN1 expression is associated with HIV infections. PMID: 29643244
3. Thus, AID is a conserved functional domain in SUN proteins and this work provides the structural evidence to support the conversation of the AID-mediated autoinhibition of SUN proteins. PMID: 29408528
4. SUN1/SUN2 may function redundantly in early HIV-1 infection steps and therefore influence HIV-1 replication and pathogenesis. PMID: 28747499
5. wndchrm revealed a consistent negative correlation between SUN1 expression and the size of nucleoli in human breast cancer tissues. PMID: 26962703
6. Nuclear envelope associated endosome-mediated transfer depends on the nuclear envelope proteins SUN1 and SUN2, as well as the Sec61 translocon complex. PMID: 26356418
7. SUN1 plays a role in hnRNP-involved mRNA export. PMID: 26476453
8. An important role for SUN1 and SUN2 in muscle disease pathogenesis. PMID: 25210889
9. these data support a model whereby mitotic phosphorylation of SUN1 disrupts interactions with nucleoplasmic binding partners, promoting disassembly of the nuclear lamina and, potentially, its chromatin interactions PMID: 25482198
10. The Caenorhabditis elegans lamin, LMN-1, is required for nuclear migration and interacts with the nucleoplasmic domain of the SUN protein UNC-84. PMID: 25057012
11. Results highlight the interactions at the nuclear envelope where mutations in the EMD and TMPO gene in combination with mutations in SUN1 have an impact on several components of the network. PMID: 24375709
12. Farnesylation of progerin enhances its interaction with SUN1 and reduces SUN1 mobility. PMID: 24522183
13. Codepletion of SUN1/2 slows cell proliferation and results in an accumulation of morphologically defective and disoriented mitotic spindles. PMID: 24662567
14. Reduction of SUN1 overaccumulation in LMNA mutant fibroblasts and in cells derived from HGPS patients corrected nuclear defects and cellular senescence. PMID: 22541428
15. These results demonstrate that the interplay between SUN1 and farnesylated prelamin A contributes to nuclear positioning in human myofibers and may be implicated in pathogenetic mechanisms. PMID: 21311568
16. POM121 and Sun1 interact transiently to promote early steps of interphase nuclear pore complexes assembly PMID: 21727197
17. perturbations in lamin A-SUN1 and SUN2 protein interactions may underlie the opposing effects of EDMD and HGPS mutations on nuclear and cellular mechanics PMID: 19933576
18. Describes cloning and function of C. elegans unc-84 and cloning of human orthologs. PMID: 10375507
19. The Sun1 itself does not require functional A-type lamins for its localisation at the inner nuclear membrane in mammalian cells. PMID: 16079285
20. localization and anchoring of UNC84A is not dependent on the lamin proteins, in contrast to what had been observed for C. elegans UNC-84 PMID: 16445915
21. SUN1 and SUN2 may form a physical interaction between the nuclear envelope and the centrosome PMID: 17132086
22. chromosome de-condensation needs the function of an inner nuclear membrane (INM) protein hsSUN1 and a membrane-associated histone acetyltransferase (HAT), hALP. PMID: 17631499
23. Results suggest that Sun1 represents an important determinant of nuclear pore complex distribution across the nuclear surface. PMID: 17724119
24. Isolation of a cDNA encoding a mouse homolog of the human SUN1 (UNC84A) gene. PMID: 12036294
25. KIAA0810 is a novel 100-kDa transmembrane protein with similarity to Caenorhabditis elegans Unc-84A and resides in the inner nuclear membrane. It is likely to interact with the nuclear lamina. PMID: 11593002
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亞細胞定位：

Nucleus inner membrane; Single-pass type II membrane protein.
數據庫鏈接：

HGNC: 18587

OMIM: 607723

KEGG: hsa:23353

STRING: 9606.ENSP00000384015

UniGene: Hs.438072

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