RALBP1 Recombinant Monoclonal Antibody

說明書

中文名稱：

RALBP1 Recombinant Monoclonal Antibody
貨號：

CSB-RA065704A0HU
規(guī)格：

￥1320
圖片：
Flow cytometric analysis of RALBP1 expression in HeLa cells using RALBP1 antibody. Green, isotype control; red, RALBP1.

Immunocytochemical staining of Hela cells with RALBP1 antibody. Nuclei were stained blue with DAPI; RALBP1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using RALBP1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RALBP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

Uniprot No.：

Q15311
基因名：

RALBP1
別名：

RalA Binding Protein 1; RLIP76; RIP1; Dinitrophenyl S-Glutathione ATPase; 76 KDa Ral-Interacting Protein; Ral-Interacting Protein 1; RalA-Binding Protein 1; DNP-SG ATPase; RLIP1; RIP; EC 7.6.2.2; EC 7.6.2.3; RalBP1; RLIP
反應(yīng)種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human RALBP1 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

25A4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:2000-1:10000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Multifunctional protein that functions as a downstream effector of RALA and RALB. As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity. As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis. During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle. During mitosis, also controls mitochondrial fission as an effector of RALA. Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L.; Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance.
基因功能參考文獻：
1. RLIP76 plays a critical role in cellular proliferation, apoptosis, cell cycle distribution, cell movement and invasion in melanoma PMID: 28537681
2. In the exons and exon-intron boundaries of ABCB5 and RLIP76 genes. PMID: 26975227
3. the miR143-3p level was markedly lower in participants with ovarian cancer compared with normal control, while the expression of RALBP1 mRNA and protein were evidently overexpressed in participants with ovarian cancer compared with normal control. PMID: 27748916
4. RLIP76 knockdown increased autophagic flux and apoptosis in U251 glioma cells. PMID: 27473470
5. Phosphorylation level of Akt declined from 138.45+/-13.8 to 69.9+/-29.7% in SGC-7901, and from 115.5+/-26.6 to 49.07+/-27% in MGC-803 and phosphorylation level of mTOR also significantly decreased.While apoptosis of gastric cancer(GA) cells increased which we verified with apoptosis proteins and staining analysis. Our data showed that RLIP76 plays a significant oncogenic role in GC and it maybe a potential target in GC PMID: 27572296
6. Anti-tumor effect was exerted when miR-124 directly targeted RLIP76, a stress-inducible transporter that plays a crucial role in the development of melanoma. PMID: 27657824
7. report showed that RLIP76 expression was significantly increased in breast cancer samples and positively correlated with the malignant status of breast cancer patients; results indicated that high RLIP76 expression was associated with poor prognosis of breast cancer patients PMID: 26125275
8. RLIP76 expression is induced by TNF-alpha and follows the induction kinetics of inflammation markers, suggesting that inflammation can influence RLIP76 expression at the blood brain barrier. PMID: 26406496
9. RLIP76 downregulation in HT29 CRC cells suppressed cell growth, enhanced cell apoptosis, induced cell cycle arrest, and inhibited cell invasion by decreasing MMP2 expression. PMID: 25213293
10. High RLIP76 expression is associated with a poor outcome of meningioma. PMID: 25993541
11. results revealed that the effect of miR-101 on prostate cancer cell apoptosis was due to RLIP76 regulation of the PI3K/Akt/Bcl-2 signaling pathway PMID: 26067553
12. RalBP1 protein is an independent predictor of poor survival and early relapse for CRC patients PMID: 22549157
13. RLIP76 is a potential target for developing novel therapeutic strategies for leukemia PMID: 24839008
14. RLIP76 is a node for Rho and Ras family signalling. [Review] PMID: 24450627
15. Activation of RalBP1 during neoplastic epithelial cell transformation induces cytoplasmic accumulation of p27, this event requires p27 Ser-10 phosphorylation by protein kinase B/Akt. PMID: 23576547
16. RLIP76 may suppress apoptosis and promote the proliferation of glioma cells by direct adenosine triphosphate-dependent xenobiotic transport and by activating the Rac1-JNK signaling pathway. PMID: 23276796
17. p300 associates with the RLIP76 promoter via an overlapping cMYB and cETS binding site and regulates RLIP76 promoter activity and its expression. PMID: 23419874
18. RalB-mediated invadopodium formation was dependent on RalBP1/RLIP76; disruption of the ATPase function of RalBP1 impaired invadopodium formation. PMID: 22331470
19. The impairment of RLIP76 by aaRLIP76 can play a role in the damage of vascular cells from females, contributing to the gender-associated pathogenesis of immune-mediated vascular diseases. PMID: 21671802
20. Data show that disrupting either RALA or RALBP1 leads to a loss of mitochondrial fission at mitosis, improper segregation of mitochondria during cytokinesis and a decrease in ATP levels and cell number. PMID: 21822277
21. studies suggest that the expression of RalBP1 is necessary for human cancer cell metastasis; show that the requirement for RalA expression for manifestation of this phenotype is not entirely dependent on a RalA-RalBP1 interaction PMID: 21170262
22. a link between RLIP76 mediated GS-E transport and cell cycle signaling are presented. PMID: 20183533
23. Studies offer strong support for the hypothesis that RLIP76 is an overarching anti-apoptosis mechanism that, if inhibited, can be more broadly effective in the treatment of renal cell carcinoma. PMID: 19626587
24. We have compared the transport properties of recombinant RLIP76 and human erythrocyte membrane RLIP76. PMID: 11732624
25. In this review, RLIP76-mediated transport of organic ions has physiological and toxicological relevance which may play an important role in the mechanism of drug resistance. PMID: 12433796
26. RLIP76 activity is a general determinant of 4HNE and DOX resistance. Its activity contributes to the drug-resistant phenotype of NSCLC. PMID: 12527936
27. RLIP76 has a role in Doxorubicin transport in lung cancer PMID: 12632060
28. RLIP76 has a role in triggering apoptosis in lung cancer cells and synergistically increaseing doxorubicin cytotoxicity PMID: 12632061
29. RLIP/RalBP1 is used as a platform by the mitotic cdk1 to facilitate the phosphorylation of Epsin, which makes Epsin incompetent for endocytosis during mitosis, when endocytosis is switched off. PMID: 12775724
30. These results show for the first time that POB1 can regulate the transport function of RLIP76 and are consistent with our previous studies showing that inhibition of RLIP76 induces apoptosis in cancer cells. PMID: 15707977
31. Results identify targets in RLIP76 for phosphorylation by protein kinase C alpha, which may act as substrates for differential transport of doxorubicin. PMID: 16087181
32. RLIP76 is the predominant transporter of antiepileptic drugs in the blood brain barrier and may be involved in mechanisms of drug-resistant epilepsy. PMID: 16188027
33. Augmenting cellular levels of RLIP76 using purified recombinant RLIP76 increased growth rate in all cells, and restored the sensitivity of RLIP76-/- mouse embryonic fibroblasts to both inhibition through PKCalpha-depletion and stimulation through PMA. PMID: 16890208
34. identify a role for caspase-8 in monocytes undergoing macrophagic differentiation, that is, the enzyme activated in an atypical complex down-regulates NF-kappaB activity through RIP1 cleavage. PMID: 17047155
35. phosphoprotein mapping of Ral binding protein 1 (RalBP1/Rip1/RLIP76) PMID: 17706599
36. the spatiotemporal mobilization of TICAM-1 in response to dsRNA and the formation of the TICAM-1 speckles containing RIP1 and NAP1 are important for the activation of the TLR3-TICAM-1 pathway. PMID: 17982077
37. autoantibodies to RLIP76 play a pathogenetic role in immune-mediated vascular diseases PMID: 17993611
38. common variants in RLIP76 are unlikely to contribute to epilepsy drug response. PMID: 18086001
39. Hsf-1 causes specific and saturable inhibition of the transport activity of Ralbp1 and that the combination of Hsf-1 and POB1 causes nearly complete inhibition through specific bindings with Ralbp1. PMID: 18474607
40. RLIP76 is a fundamental link between biochemical pathways and glutathione-linked metabolism of xenobiotics and stress-degense signaling pathways. PMID: 18628450
41. RLIP76 serves a key effector function for survival of prostate cancer cells; depletion of RLIP76 in mice bearing xenografts of prostate cancer cells leads to near complete regression of established subcutaneous xenografts with no apparent toxic effects. PMID: 19073149
42. the accumulation-deficient drug-resistance mediated by RLIP76 can be modulated by inhibition of RLIP76 transport activity by cdc2. PMID: 19375851
43. RLIP76 is an anticancer for kidney cancer: inhibition of RLIP76 function by antibody or its depletion by small interfering RNA or antisense DNA causes marked regression of kidney xenografts in nude mice. PMID: 19417134
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亞細胞定位：

Cell membrane; Peripheral membrane protein. Cytoplasm, cytosol. Cytoplasm, cytoskeleton, spindle pole. Nucleus. Mitochondrion.
組織特異性：

Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
數(shù)據(jù)庫鏈接：

HGNC: 9841

OMIM: 605801

KEGG: hsa:10928

STRING: 9606.ENSP00000019317

UniGene: Hs.528993

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