PSMD4 Recombinant Monoclonal Antibody

說明書

中文名稱：

PSMD4 Recombinant Monoclonal Antibody
貨號：

CSB-RA226285A0HU
規(guī)格：

￥1320
圖片：
Flow cytometric analysis of PSMD4 expression in HepG2 cells using PSMD4 antibody. Green, isotype control; red, PSMD4.

Immunocytochemical staining of HepG2 cells with PSMD4 antibody. Nuclei were stained blue with DAPI; PSMD4 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using PSMD4 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PSMD4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P55036
基因名：

PSMD4
別名：

PSMD4; Proteasome 26S Subunit Ubiquitin Receptor, Non-ATPase 4; AF; Rpn10; AF-1; S5A; Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4; 26S Proteasome Non-ATPase Regulatory Subunit 4; 26S Proteasome Regulatory Subunit S5A; Proteasome 26S Subunit, Non-ATPase 4; Multiubiquitin Chain-Binding Protein; Antisecretory Factor 1; MCB1; ASF; 26S Proteasome Regulatory Subunit RPN10; S5a/Antisecretory Factor Protein; Angiocidin; PUB-R5
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human PSMD4 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

17F12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
基因功能參考文獻：
1. Preterm delivery is associated with low levels of anti-secretory factor in placenta. Inflammation, a potential trigger of preterm birth, is more pronounced in the preterm placenta and inversely related to the placental level of anti-secretory factor. PMID: 29265188
2. We therefore suggest that PSMD4 or b-catenin might be potential targets for suppressing tumor aggressiveness, and consequently, improving outcomes in patients whose tumors express cNrf2. PMID: 27033953
3. Binding (especially high-affinity binding) of non-ubiquitinated proteins to the Rpn10 proteasome subunit can both regulate the functioning of this proteasomal ubiquitin receptor (by competing with ubiquitinated substrates) and promote activation of other pathways for proteolytic degradation of proteins destined to the proteasome. PMID: 28988533
4. The platelet 26S proteasome exhibits different basal activities of its catalytic subunits and chymotrypsin-like activity is most prominently enhanced by calcium dependent signaling. Collagen stimulation enhances 26S proteasome chymotrypsin-like activity in platelets. PMID: 27768934
5. regulation of NY-ESO-1 processing by the ubiquitin receptors Rpn10 and Rpn13 as a well as by the standard and immunoproteasome is governed by non-canonical ubiquitination on non-lysine sites. PMID: 26903513
6. Not only AF1, but an entire proteasome complex, seems to be present in blood. PMID: 25897558
7. binds to death receptor-6, and induces monocyte cell line differentiation via NF-kB pathway PMID: 24829148
8. The VWA domain regulation of ubiquitin and Ubl binding to S5a is restricted to the 26S proteasome. PMID: 25318673
9. The degradation of TP53 and MDM2 by the proteasome can be selectively dependent on S5a in human cells. PMID: 24121268
10. To examine angiocidin expression in SMMC-7221 and HepG2 cells and the role of angiocidin in liver cancer cell growth. Angiocidin is highly expressed in liver cancer cells, and it may play a key role in tumor growth of liver cancers. PMID: 24250289
11. Authors demonstrate that human cytomegalovirus UL76 induces a novel nuclear aggresome, likely by subverting S5a of the ubiquitin-proteasome system. PMID: 23966401
12. One consequence of the interaction between E6/E6AP and S5a is enhanced ubiquitination of this proteasome subunit. PMID: 24074603
13. These studies suggest that diminished 26S activity in failing human hearts may be related ti impaired docking of the 19S to the 20S as a result of decreased Rpt subunit ATPase activity and alpha7 subunit phosphorylation. PMID: 23515276
14. identified the VWA domain of hRpn10 as a receptor for ubiquitin-like proteins within the 26S proteasome and elucidated how FAT10 mediates efficient proteolysis by the proteasome PMID: 22434192
15. Both higher PSMD4 expression levels and higher 1q21 copy numbers affected clinical outcome adversely. PMID: 21628408
16. results suggest that there is different substrate specificity between S5a and hRpn13 at the level of delivery and S5a may be the major docking site for ERAD substrates. PMID: 20417181
17. Overexpression of the S5a subunit of proteasome activator PA700 did not recover proteasome function in Huntington disease cells. S5a. PMID: 17327906
18. parkin Ubld uses differential surfaces to recruit UIM regions from the S5a proteasomal subunit compared with Eps15 involved in cell signaling. PMID: 19875440
19. S5a component of the 19S complex interacts with different ubiquitin-like (ubl) modules PMID: 11827521
20. HHr23a binds to this protein, which changes its own conformation. PMID: 14557549
21. structure of the ubiquitin-interacting motif of the proteasome subunit S5a bound to the ubiquitin-like domain of HR23B PMID: 14585839
22. Results report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules, and present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin. PMID: 15826667
23. These results are consistent with the conclusion that the anti-tumor activity of angiocidin arises from its ability to ligate collagen and alpha2beta1 on endothelial cells and tumor cells. PMID: 16762342
24. UIM2 domain of S5a binds preferentially to hHR23a over polyubiquitin, and a model is provided for the ternary complex that represents one of the mechanisms used by the proteasome to capture ubiquitylated substrates. PMID: 17408689
25. results suggest that S5a is regulated during apoptosis at the transcriptional level and S5a upregulation by antiapoptotic signals can contribute to cell survival. PMID: 17459097
26. S5a-UIMs can be used as upstream inhibitors of the proteasome pathway. PMID: 17949686
27. In autoimmune disorders like MS, angiocidin activates T cells, macrophages, microglia & astrocytes to produce inflammatory cytokines and to stimulate antigen presentation. PMID: 18207252
28. angiocidin activates monocytes to secrete cytokines and differentiates them to a macrophage-like phenotype through at least two pathways mediated by MAPK and NF-kappaB, as well as PI3K PMID: 18632645
29. The presence of S5a in the reaction containing a substrate (luciferase), ubiquitination enzymes (an E2, UbcH5, and the U-box E3 ligase CHIP) and 26S proteasome significantly increased the rate of luciferase degradation. PMID: 19387488
30. Angiocidin's ability to elicit tumor cell death may be mediated in part by it's pro-inflammatory effects on immune cells in the tumor microenvironment. PMID: 19519434
31. The binding of S5a to K48-linked diubiquitin is defined at an atomic level resolution. PMID: 19683493
32. Proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (also called subunit 5a) has two independent polyubiquitin binding sites whose sequences are highly conserved among higher eukaryotic orthologs. PMID: 9488668
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蛋白家族：

Proteasome subunit S5A family
數據庫鏈接：

HGNC: 9561

OMIM: 601648

KEGG: hsa:5710

STRING: 9606.ENSP00000357879

UniGene: Hs.505059

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