PRKAR2A Recombinant Monoclonal Antibody
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中文名稱：PRKAR2A重組抗體
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貨號：CSB-RA197122A0HU
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規格：￥1320
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圖片：
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IHC image of CSB-RA197122A0HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB. -
IHC image of CSB-RA197122A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB. -
Immunofluorescence staining of Hela with CSB-RA197122A0HU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 512-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing MCF-7 cells stained with CSB-RA197122A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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產品描述：PRKAR2A Recombinant Monoclonal Antibody（Code: CSB-RA197122A0HU）靶向cAMP依賴性蛋白激酶A的調節亞基2A（PRKAR2A），該蛋白在介導cAMP信號通路中發揮關鍵作用，參與調控細胞代謝、基因表達及分化等生物學過程。本抗體經ELISA、免疫組化（IHC）、免疫熒光（IF）和流式細胞術（FC）嚴格驗證，適用于多種實驗場景：IHC推薦稀釋度1:50-1:200，可清晰顯示組織切片中PRKAR2A的定位與表達水平；IF在相同稀釋范圍內能精準標記細胞內靶蛋白的亞細胞分布；FC分析則支持檢測細胞表面或胞內PRKAR2A的表達動態。該重組單抗具有高親和力和特異性，適用于探索PRKAR2A在細胞信號轉導、代謝性疾病及腫瘤微環境中的功能機制研究，為體外模型構建、蛋白互作分析及病理生理學研究提供可靠工具。產品嚴格限于科研用途，建議根據實驗體系優化工作濃度。
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Uniprot No.：
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基因名：
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別名：cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2A, PKR2 PRKAR2
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反應種屬：Human
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免疫原：A synthesized peptide derived from Human PRKAR2A
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：15G8
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
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基因功能參考文獻：
- High PK-R2 expression is associated with colorectal cancer. PMID: 26372733
- Prkar2a deficiency predisposes to hematopoietic malignancies in vivo. RIIalpha's likely association with HS and DLBCL was hitherto unrecognized and may lead to better understanding of these rare neoplasms. PMID: 26608815
- Disruption of Snapin-PKR2 interaction did not affect PKR2 signaling, but increased the ligand-induced degradation, implying a role of Snapin in the trafficking of PKR2. PMID: 26687946
- we demonstrate that neurochondrin has strong isoform selectivity towards the RIIa subunit of PKA with nanomolar affinity PMID: 25916936
- These data demonstrate that some Kallmann syndrome-associated, intracellularly retained mutant PKR2 receptors can be functionally rescued, suggesting a potential treatment strategy for patients bearing such mutations. PMID: 24753254
- Smad4 and the R subunit of the protein kinase A holoenzyme form a functional complex in vivo in response to TGFbeta. PMID: 23362281
- ETO nervy homology region (NHR) 3 domain-PKA(RIIalpha) protein interaction does not appear to significantly contribute to AML1-ETO's ability to induce leukemia. PMID: 20708017
- findings indicate that increased particulate type II protein kinase A activity occurs throughout pregnancy therefore directing the cAMP quiescence signal to specific subcellular loci within myometrial smooth muscle cells PMID: 12727975
- These data implicate the involvement of PKA-RIIalpha anchoring apical targeting of distinct proteins and glycosphingolipids to apical plasma membrane domains and suggest that rerouting may underlie the delayed Golgi-to-apical surface transport of MDR1. PMID: 16723498
- The high-resolution crystal structures of the docking and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA in complex with the high-affinity anchoring peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit recognition. PMID: 17081989
- The data suggest that centrosomal anchoring of RIIalpha and the interrelated subapical positioning of these centrosomes is required for oncostatin M-, but not cAMP-mediated, bile canalicular lumen development. PMID: 17494870
- RIIalpha releases Calpha upon elevated cAMP alone, dependent on autophosphorylation of the RIIalpha inhibitory domain PMID: 17884635
- Bacillus anthracis edema toxin altered the protein levels and activity of protein kinase A and exchange protein activated by cAMP (Epac), a recently identified cAMP-binding molecule. PMID: 19307216
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亞細胞定位：Cytoplasm. Cell membrane. Note=Colocalizes with PJA2 in the cytoplasm and the cell membrane.
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蛋白家族：CAMP-dependent kinase regulatory chain family
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組織特異性：Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
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數據庫鏈接：
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