PLSCR3 Recombinant Monoclonal Antibody

說明書

中文名稱：

PLSCR3 Recombinant Monoclonal Antibody
貨號：

CSB-RA789286A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of phospholipid scramblase 3 expression in H9c2 cells using phospholipid scramblase 3 antibody. Green, isotype control; red, phospholipid scramblase 3.

Immunocytochemical staining of H9c2 cells with Phospholipid scramblase 3 antibody. Nuclei were stained blue with DAPI;Phospholipid scramblase 3 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using phospholipid scramblase 3 antibody. Total cell lysates (20 μg for H9c2 and C2C12, 30 μg for others ) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospholipid scramblase 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q9NRY6
基因名：

PLSCR3
別名：

PLSCR3; Phospholipid Scramblase 3; Ca(2+)-Dependent Phospholipid Scramblase 3; PL Scramblase 3
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human PLSCR3 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

13E11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of the phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer membrane of the mitochondria. Plays an important role in mitochondrial respiratory function, morphology, and apoptotic response. Mediates the translocation of cardiolipin from the mitochondrial inner membrane to outer membrane enhancing t-Bid induced cytochrome c release and apoptosis. Enhances TNFSF10-induced apoptosis by regulating the distribution of cardiolipin in the mitochondrial membrane resulting in increased release of apoptogenic factors and consequent amplification of the activity of caspases. Regulates cardiolipin de novo biosynthesis and its resynthesis.
基因功能參考文獻：
1. The results revealed that Ca2+ and Mg2+ bind to Human phospholipid scramblase 3 (hPLSCR3) and trigger conformational changes mediated by aggregation. PMID: 29337693
2. Secreted Scr3 was taken up by HeLa cells, suggesting that Scr3 functions as a cell-to-cell transferable modulator carried by exosomes in a paracrine manner. PMID: 23350699
3. PLS3 is a downstream effector of PKC-delta in the mitochondria. PMID: 12649167
4. PLS3 as a critical regulator of mitochondrial structure and respiration, and CL transport in apoptosis. PMID: 14573790
5. This enzyme is a novel regulator of cardiolipin de novo biosynthesis and its resynthesis. PMID: 16939411
6. These findings indicate that phosphorylation of PLS3 by PKC-delta induces PLS3 activation to facilitate mitochondrial targeting of tBid and apoptosis. PMID: 17226776
7. Identification of Alix-type and Non-Alix-type ALG-2-binding sites in human phospholipid scramblase 3: differential binding to an alternatively spliced isoform and amino acid-substituted mutants PMID: 18256029
8. TRAIL-induced activation of PKC-delta mediates regulation of the phospholipid scramblase3-induced changes in cardiolipin. PMID: 18491232
顯示更多

收起更多
亞細胞定位：

Mitochondrion membrane; Single-pass type II membrane protein. Mitochondrion inner membrane; Single-pass type II membrane protein. Nucleus.
蛋白家族：

Phospholipid scramblase family
組織特異性：

Expressed in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, uterus, small intestine and peripheral blood lymphocytes. Not detected in testis, brain and liver.
數據庫鏈接：

HGNC: 16495

OMIM: 607611

KEGG: hsa:57048

STRING: 9606.ENSP00000316021

UniGene: Hs.534591

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WDR5 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human