PLAA Recombinant Monoclonal Antibody
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中文名稱：PLAA Recombinant Monoclonal Antibody
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貨號：CSB-RA565057A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30μg), U-251MG whole cell lysate(30μg), HEK293T whole cell lysate(30μg), HT-29 whole cell lysate(30μg), A431 whole cell lysate(30μg)
All lanes: PLAP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time：1min40s -
Immunofluorescence staining of Hela cell with CSB-RA565057A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing Hela cells stained with CSB-RA565057A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：Phospholipase A-2-activating protein (PLA2P) (PLAP), PLAA, PLAP
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反應種屬：Human
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免疫原：A synthesized peptide from human PLAA protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：1F3
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Plays a role in protein ubiquitination, sorting and degradation through its association with VCP. Involved in ubiquitin-mediated membrane proteins trafficking to late endosomes in an ESCRT-dependent manner, and hence plays a role in synaptic vesicle recycling. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. Plays a role in cerebellar Purkinje cell development. Positively regulates cytosolic and calcium-independent phospholipase A2 activities in a tumor necrosis factor alpha (TNF-alpha)- or lipopolysaccharide (LPS)-dependent manner, and hence prostaglandin E2 biosynthesis.
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基因功能參考文獻：
- In both human and mouse, hypomorphic mutations in the ubiquitin adaptor protein PLAA cause an infantile-lethal neurodysfunction syndrome with seizures. PMID: 28413018
- This study presented that Phospholipase A2-activating protein is associated with a novel form of leukoencephalopathy. PMID: 28007986
- This review provides evidence that 1,25-dihydroxy vitamin D3 stimulates PLA2 via PLAA and CaMKII, a process initiated by Pdia3/PLAA interaction, which further triggers CaMKII-dependent PLA2 activation. [review] PMID: 25448737
- Function assignment to conserved residues in mammalian alkaline phosphatases. PMID: 11937510
- PLAP inhibits sPLA2-triggered release of fatty acids from erythrocyte membranes PMID: 14499668
- identified one stimulatory element, with Sp1 binding sites, and one inhibitory element, in exon 1 of the plaa gene PMID: 18291623
- Structural basis for ubiquitin recognition by a novel domain from the human phospholipase A2-activating protein PMID: 19423704
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相關疾病：Neurodevelopmental disorder with progressive microcephaly, spasticity, and brain anomalies (NDMSBA)
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亞細胞定位：Nucleus. Cytoplasm. Cell junction, synapse.
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蛋白家族：WD repeat PLAP family
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數據庫鏈接：
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