NUMA1 Recombinant Monoclonal Antibody

說明書

中文名稱：

NUMA1重組抗體
貨號：

CSB-RA182340A0HU
規(guī)格：

￥1320
圖片：
Overlay Peak curve showing Hela cells stained with CSB-RA182340A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA182340A0HU NUMA1重組單克隆抗體是針對核有絲分裂器蛋白1（NUMA1）的高特異性科研試劑，該靶標蛋白在維持細胞核結構完整性及調控有絲分裂紡錘體組裝中起關鍵作用，是研究細胞分裂機制與核動力學的重要分子。本產品采用重組表達技術制備，經(jīng)ELISA與流式細胞術（FC）雙重驗證，其中FC應用推薦使用1:50至1:200的稀釋范圍，可精準檢測內源性NUMA1蛋白在細胞周期中的動態(tài)定位及表達水平變化。適用于細胞生物學領域中對有絲分裂進程的實時追蹤、核膜重組研究以及紡錘體極性分析等實驗場景，亦可用于腫瘤細胞異常分裂機制或特定疾病模型中核結構紊亂的關聯(lián)性探索。該抗體嚴格限于基礎科研用途，能夠為體外培養(yǎng)細胞的蛋白功能研究提供可靠工具，滿足活細胞成像、細胞周期同步化分析等實驗需求。
Uniprot No.：

Q14980
基因名：

NUMA1
別名：

Nuclear mitotic apparatus protein 1 (Nuclear matrix protein-22) (NMP-22) (Nuclear mitotic apparatus protein) (NuMA protein) (SP-H antigen), NUMA1, NMP22 NUMA
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human NUMA1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

12A11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division. Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles. Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner. In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle. During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation. Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro. Also required for proper orientation of the mitotic spindle during asymmetric cell divisions. Plays a role in mitotic MT aster assembly. Involved in anastral spindle assembly. Positively regulates TNKS protein localization to spindle poles in mitosis. Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume. Required for epidermal differentiation and hair follicle morphogenesis.
基因功能參考文獻：
1. It has been reported that the Galectin-3/NuMA interaction is functionally important for the spindle pole organization; spindle pole cohesion requires glycosylation-mediated localization of NuMA. PMID: 28469279
2. The p37 negatively regulates this function of PP1, resulting in lower cortical NuMA levels and correct spindle orientation. PMID: 29222185
3. The results show how E-cadherin instructs the assembly of the LGN/NuMA complex at cell-cell contacts, and define a mechanism that couples cell division orientation to intercellular adhesion. PMID: 28045117
4. Here, the authors use quantitative imaging and laser ablation to show that NuMA targets dynactin to spindle microtubule minus-ends, localizing dynein activity there. PMID: 29185983
5. All urine samples were analyzed by voided urine and bladder washing cytology, NMP22 and UBC rapid test (qualitatively and quantitatively). The best cutoff (highest Youden index; >/=6.7 ng/ml) for the quantitative UBC was determined by receiver operating characteristic curves. PMID: 28824318
6. Short isoform of NuMA might be functioned as a putative role of tumor suppressor. Further studies should be made to illuminate the relationship between ACTN4, MYBL2, and tumor progression. PMID: 28748856
7. The function of nuclear mitotic apparatus protein (NuMA) in rDNA transcription and p53-independent nucleolar stress response suggests a central role for NuMA in cellular homeostasis. PMID: 28981686
8. Importin-alpha/-beta regulates the NuMA functioning required for assembly of higher-order microtubule structures including the mitotic spindle. PMID: 28939615
9. Low post translational modifications of NuMA protein is associated with neoplasms. PMID: 28209915
10. Seven NuMA isoforms generated by alternative splicing were categorized into 3 groups: long, middle and short. Both exons 15 and 16 in long NuMA were "hotspot" for alternative splicing. Lower expression of short NuMA was observed in cancer cells compared with nonneoplastic controls. PMID: 25451259
11. Chimeric proteins constructed by fusion of LANA of Kaposi's sarcoma-associated herpesvirus with the NuMA could bind with ori-P and enhance replication of an ori-P-containing plasmid. PMID: 27829174
12. findings reveal a direct physical link between two important regulators of mitotic progression and demonstrate the critical role of the NuMA-Astrin interaction for accurate cell division. PMID: 27462074
13. Aurora-A governs the dynamic exchange between the cytoplasmic and the spindle pole-localized pools of NuMA. Aurora-A phosphorylates directly the C terminus of NuMA on three Ser residues, of which Ser1969 determines the dynamic behavior and the spindle orientation functions of NuMA. PMID: 26832443
14. Suppressor APC domain containing 2 negatively regulates the localization of LGN at the cell cortex, likely by competing with NuMA for its binding PMID: 26766442
15. Letter: risk factors for false positive results when using urinary NMP22 as biomarker for early detection of bladder cancer. PMID: 24976592
16. Results show that at low grade of disease, NMP22 test provided a significantly higher sensitivity for the detection of recurrent urothelial carcinoma of the bladder compared to voided urine cytology specimens. PMID: 25488052
17. NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane.During anaphase correct NuMA localization is mediated by direct membrane phospholipid binding. PMID: 24996901
18. Retinoblastoma protein (pRB) have a novel function in regulating the mitotic function of NuMA and spindle organization, which are required for proper cell cycle progression. PMID: 24350565
19. Study finds that frictional forces increase nonlinearly with microtubule-associated proteins (MAP) velocity across microtubules and depend on filament polarity, with NuMA's friction being lower when moving toward minus ends, EB1's lower toward plus ends, and PRC1's exhibiting no directional preference. PMID: 24725408
20. The mitosis-dependent dynamic SUMO-1 modification of NuMA might contribute to NuMA-mediated formation and maintenance of mitotic spindle poles during mitosis. PMID: 24309115
21. ectopic expression of NuMA can manipulate endogenous p53 and p21 transcriptional expression during interphase. PMID: 23828576
22. ectopic expression of BRAP2 inhibits nuclear localization of HMG20A and NuMA1, and prevents nuclear envelope accumulation of SYNE2. PMID: 23707952
23. Hepatocyte Par1b defines lumen position in concert with the position of the astral microtubule anchoring complex LGN-NuMA to yield the distinct epithelial division phenotypes. PMID: 24165937
24. NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function. PMID: 23921553
25. Phosphorylation of NuMA by aurora-A is important for cell survival. PMID: 23097092
26. Data indicate that dynein- and astral microtubule-mediated transport of Galphai/LGN/nuclear mitotic apparatus (NuMA) complex from cell cortex to spindle poles. PMID: 23389635
27. nuclear matrix protein 22 (nuclear mitotic apparatus protein, NuMA) has a role in upper tract urothelial tumors. PMID: 21865670
28. NuMA is required for the recruitment of cyclin-dependent kinase 8, a component of the Mediator complex and a promoter of p53-mediated p21 gene function. PMID: 23589328
29. Studies indicate that the Inscuteable (Insc)and NuMA are mutually exclusive interactors of LGN. PMID: 22977735
30. Numa regulates spindle assemby in conjunction with Eg5. PMID: 23368718
31. NuMA expression was upregulated in tumours, with a significant association with disease stage in mucinous EOC subtypes, lymph node involvement and patient age PMID: 22719996
32. Low NUMA1 is associtated with glioblastoma. PMID: 22619067
33. Without functional NuMA, microtubules lose connection to meiosis I spindle poles, resulting in highly disorganized early spindle assembly. PMID: 22552228
34. During apoptotic rearrangement of interchromatin granule clusters, the nuclear matrix (NuMa rearrangement) and chromatin are closely associated. This process occurs in defined stages and depends on the activity of protein phosphatases, caspases and CAD. PMID: 22023725
35. The levels of urinary NMP22 and CK18 in the patients with transitional cell carcinoma of the bladder were significantly higher than those in the non-transitional cell carcinoma of the bladder. PMID: 19615282
36. Accurate distribution of NuMA is important for oocyte maturation, zygote and embryo development in humans. Proper assembly of NuMA is likely necessary for bipolar spindle organization and human oocyte developmental competence. PMID: 21297155
37. NuMA is expressed in interphase nuclei of fibroblasts and oocytes. PMID: 21406448
38. Phenotype onset is correlated with NuMA-RARalpha copy number; mice with higher copy number developing disease later than those with lower copy number. PMID: 21255834
39. These results suggest that NuMA may provide structural support in the interphase nucleus by contributing to the organization of chromatin. PMID: 20467816
40. Ric-8A and Gi alpha recruit LGN, NuMA, and dynein to the cell cortex to help orient the mitotic spindle. PMID: 20479129
41. Data suggest that pADPr provides a dynamic cross-linking function at spindle poles by extending from covalent modification sites on PARP-5a and NuMA and binding noncovalently to NuMA and that this function helps promote assembly of exactly two poles. PMID: 19759176
42. A domain within the C-terminal tail of NuMA interacts with tubulin and induces bundling and stabilisation of microtubules and leads to formation of abnormal mitotic spindles. PMID: 11956313
43. NuMA is cleaved differently in Jurkat T and HeLa cells, suggesting that different sets of caspases are activated in these cell lines. The normal diffuse intranuclear distribution of NuMA changed during apoptosis. PMID: 12508117
44. role in development of myelodi leukemia with promyelocytic features PMID: 14737102
45. Proteins and open reading frames with a NuMA C terminus distal portion like region were found in a diverse set of vertebrate species including mammals, birds, amphibia, and early teleost fish. PMID: 15388855
46. Multiple mechanisms regulate NUMA1 dynamics at spindle poles. PMID: 15561764
47. concluded that variations in the NuMA gene are likely responsible for the observed increased breast cancer risk PMID: 15684076
48. NuMA plays diverse important roles in vertebrate cells [review] PMID: 16146802
49. NuMA has a role in mammary epithelial differentiation by influencing the organization of chromatin. PMID: 17108325
50. point to the Rae1-NuMA interaction as a critical element for normal spindle formation in mitosis PMID: 17172455
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亞細胞定位：

Nucleus. Nucleus, nucleoplasm. Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cell cortex. Cell membrane; Lipid-anchor; Cytoplasmic side. Lateral cell membrane.; [Isoform 3]: Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.; [Isoform 4]: Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
數(shù)據(jù)庫鏈接：

HGNC: 8059

OMIM: 164009

KEGG: hsa:4926

STRING: 9606.ENSP00000377298

UniGene: Hs.325978

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