NRP1 Recombinant Monoclonal Antibody

說明書

中文名稱：

NRP1重組抗體
貨號：

CSB-RA016091MA1HU
規格：

￥1320
圖片：
Overlay Peak curve showing PC3 cells stained with CSB-RA016091MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA016091MA1HU重組單克隆抗體是針對神經纖毛蛋白-1（NRP1）開發的高特異性科研工具。NRP1作為多功能跨膜受體，在血管生成、細胞遷移及免疫調控等生物學過程中發揮重要作用，其異常表達與多種病理生理機制密切關聯。本產品經ELISA和流式細胞術（FC）嚴格驗證，在FC應用中推薦使用1:50-1:200稀釋比例，可精準檢測細胞表面NRP1蛋白表達水平。抗體采用重組技術生產，具有優異的批間一致性，且通過抗原親和力驗證確保靶標結合可靠性。該抗體適用于體外細胞模型的功能研究，如腫瘤微環境中血管內皮細胞與免疫細胞的相互作用分析、細胞信號轉導機制探索以及相關受體配體結合實驗等基礎研究領域。科研人員可借助本產品開展NRP1在腫瘤血管形成、免疫細胞調控及組織發育等方向的作用機制研究，為相關疾病的分子機制解析提供實驗支持。
Uniprot No.：

O14786
基因名：

NRP1
別名：

NRP1 Recombinant Monoclonal Antibody
反應種屬：

Human
免疫原：

Recombinant Human NRP1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號：

10C10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR.; Binds VEGF-165 and may inhibit its binding to cells. May induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.; (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection.
基因功能參考文獻：
1. HLA-G, NRP1, and PD-1, may be involved in the immune response in psoriatic patients PMID: 29790686
2. miR-320/NRP-1 axis contributes to the growth and metastasis of cholangiocarcinoma cells. PMID: 28618167
3. we demonstrated that HS3ST3B enhances the proliferation and survival of breast cancer MDA-MB-231 cells, via a mechanism that is dependent on Nrp1. PMID: 30360368
4. The serum NRP1 was significantly associated with AFP, gamma-GT, Alb, bile acid, ALT, AST, ALP and pre-Alb. NRP1 is a novel tumor marker in HCC. PMID: 29966621
5. lnc00152 was aberrantly overexpressed in colorectal tumors and cancer cells and that lnc00152 was modulated by miRNA206. lnc00152 overexpression promoted epithelial-mesenchymal transition and increased neuropilin1 expression in the colorectal cancer cells. PMID: 29956750
6. the results presented herein indicate that NRP1 exerts tumor suppressive effects in NB, at least in part by regulating the expression of beta1 integrin PMID: 29750423
7. NRP1 may be important in the etiology of menstrual migraine. PMID: 29671086
8. clinical samples from apical periodontitis patients were obtained to analyse the expression of Sema3A/Nrp1. These results indicated that the bone destruction level expanded from days 7 to 35 PMID: 29457037
9. Study demonstrated that NRP1+ cells possess tumor-initiating cells (TIC) properties, including the preferential expression of stemness markers, higher clonogenic and self-renewal potential, and increased cell migration capacity. These findings support an important role for NRP1 in the biology of lung cancer TICs. PMID: 29138851
10. Authors demonstrated that when VEGFR2 was inhibited, NRP-1 appeared to regulate RAD51 expression through the VEGFR2-independent ABL-1 pathway, consequently regulating radiation sensitivity. In addition, the combined inhibition of VEGFR2 and NRP-1 appears to sensitize cancer cells to radiation. PMID: 29777301
11. NRP1, as a promoter of odontoblast differentiation, regulates dental pulp stem cells via the classical Wnt/beta-catenin pathway. PMID: 28910136
12. The new observation that shows strong NP-1 immunoreactivity of these particles, and decreased NP1 expression in syncytiotrophoblast of preeclampsia (PET) placentas in comparison to the control group, may imply a role of NP-1 in PET. PMID: 26937865
13. rs2228638 of NRP1 may have a role in tetralogy of Fallot risk in European and Chinese Han populations PMID: 29432830
14. Data show that neuropilin 1 (NRP1) binds extracellular AGO2 (carrying miRNA or not), and internalizes AGO2/miRNA complexes. PMID: 27486976
15. Data suggest neuropilin-1 (NRP-1) as a therapeutic target to reduce tumor fibrosis and pancreatic ductal adenocarcinoma (PDAC) progression. PMID: 27542226
16. Results suggest that targeting the transmembrane domains (TMD) of neuropilin-1 (NRP1) in breast cancer is a potent new strategy to fight against breast cancer and related metastasis. PMID: 27351129
17. By blocking neuropilin-1 receptor (NRP-1) in tumor-bearing mice, luciferase activity in tumors delivered by HK polyplexes was reduced by 96%, whereas activity in normal tissues was minimally reduced. PMID: 27257039
18. High NRP1 expression is associated with metastasis in triple-negative breast cancer. PMID: 26967387
19. Overexpression of Neuropilin 1 incresed in breast cancer stem cells formation and breast cancer stem cells-induced tube formation and knockdown of Neuropilin 1 reduced CSCs-related traits. PMID: 29179185
20. Nrp1 mediates the migratory behavior of mesangial cells. PMID: 28835419
21. this study identified the NRP1 cytoplasmic domain as essential for acute vascular hyperpermeability induced by different NRP1 ligands: a ligand- blocking antibody against NRP1 and a CendR peptide. PMID: 27117252
22. Data indicate that miR-206 inhibits neuropilin-1 (NRP1) and SMAD2 gene expression by directly binding to their 3'-UTRs. PMID: 27014911
23. Results show that Nrp1 plays a critical role in balancing responsiveness to VEGF-A versus TGFbeta to regulate glioblastoma growth, progression, and recurrence after anti-vascular therapy. PMID: 28938007
24. Data provide evidence that NRP1 functions to enhance the metastatic potential of prostate tumors. PMID: 28092670
25. NRP1 is an important niche component PMID: 27676403
26. our data suggest a novel molecular mechanism by which tMUC1 may modulate NRP1-dependent VEGFR signaling in PDA cells. PMID: 26804176
27. Our findings identify aberrant active integrin b1-driven Src-Akt hyperactivation as a primary resistance mechanism to cetuximab in PDAC cells and offer an effective therapeutic strategy to overcome this resistance using an EGFR and NRP1 dual targeting antibody PMID: 27797376
28. VEGF-A acts via interaction with NRP-1 to trigger intracellular events leading to ECS cell survival and formation of aggressive, invasive and highly vascularized tumors. PMID: 26804163
29. These data identify a new molecular mechanism of brain microvascular endothelial inflammatory response through NRP1-IFNgamma crosstalk. PMID: 27591257
30. This study indicates that capsaicin application results in significant loss of epidermal NRP-1 receptor expression, whereas diabetic subjects presenting small fiber neuropathy show full epidermal NRP-1 expression in contrast to the basal expression pattern seen in healthy controls PMID: 27598321
31. Study show that a high percentage of intratumoral NRP1(+) Tregs correlates with poor prognosis in melanoma and head and neck squamous cell carcinoma. PMID: 28552348
32. Study revels the structural description of the neuropilin-1 ectopic region by reporting the crystal structure of its MAM domain. The domain adopts a jellyroll fold that is stabilized by a Ca2+ ion while forming a molecular surface that is distinct from its structural homologs. PMID: 27720589
33. Data suggest that neuropilin-1 (NRP1) functions as a receptor for neutrophil elastase (NE), mediates uptake/absorption of NE, and facilitates cross-presentation of PR1 (a peptide fragment of NE) in breast cancer cells. PMID: 28468826
34. This report is the first study characterizing the specific functions of NRP1-Delta7, a glycosaminoglycan-defective neuropilin-1 splice variant that displays anti-tumorigenic properties in vitro and in vivo. PMID: 27798666
35. this study shows that NRP1 can act as a co-receptor for PDGF-D-PDGFRbeta signaling and is possibly implicated in intercellular communication in the vascular wall PMID: 28254885
36. This review provides a general overview of current knowledge of the signalling pathways that are modulated by NRP1, with particular focus on neuronal and vascular roles in the brain and retina. PMID: 26923176
37. Curcumin-loaded nanoliposomes linked to homing peptides for integrin targeting and neuropilin-1-mediated internalization reduced viability of breast cancer cells. PMID: 27937055
38. Upregulated expression of NRP1 is associated with glioma. PMID: 27481513
39. Studies suggest that the activation of NRP-1 by PlGF directly contributes to tumour aggressiveness and to melanoma escape from anti-VEGF-A therapies. PMID: 26846845
40. REVIEW: Nrp1 functions in the vasculature is critical for the development of targeted therapeutics for cancer and vascular diseases such as atherosclerosis and retinopathies. PMID: 26849476
41. High Expression of Neuropilin-1 Associates with Hepatocellular Carcinoma. PMID: 26563279
42. Increased NRP-1 expression is associated with metastatic endometrial and lung cancers. PMID: 26701889
43. miR-152 suppression in NSCLC cells might promote neuropilin-1 mediated cancer metastasis. PMID: 26823738
44. NRP-1 was found to be overexpressed in gastric cancer (GC) tissues, and its expression correlates with the clinical staging, tumor differentiation and pathological types of gastric cancer. PMID: 26795388
45. NRP1 was targeted by miR130a and miR130b at the binding site of chromosome 10: 334668643466870, which was involved in the axon guidance signaling pathway. PMID: 26573160
46. Semaphorin-3a, neuropilin-1 and plexin-A1 are axonal guidance molecules that have been recently implicated in regulating bone metabolism. PMID: 26602825
47. neuropilin-1 is regulated in the oral epithelium and is selectively up-regulated during epithelial dysplasia PMID: 26877262
48. NRP1 over-expression in miR-365 expressing cells could rescue invasion and growth defects of miR-365. In addition, miR-365 expression inversely correlated with NRP1 protein levels in malignant melanoma PMID: 26406949
49. Data show that binding of pleiotrophin (PTN) to neuropilin-1 (NRP-1) stimulated the internalization and recycling of NRP-1 at the cell surface. PMID: 26408254
50. Study found significant down-regulation of placental NRP-1 expression in fetal growth restriction pregnancies complicated with absent end-diastolic flow in the umbilical artery. PMID: 26409917
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亞細胞定位：

[Isoform 2]: Secreted.; Mitochondrion membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Cytoplasm.
蛋白家族：

Neuropilin family
組織特異性：

[Isoform 1]: The expression of isoforms 1 and 2 does not seem to overlap. Expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and
數據庫鏈接：

HGNC: 8004

OMIM: 602069

KEGG: hsa:8829

STRING: 9606.ENSP00000265371

UniGene: Hs.131704

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