NDUFAF1 Recombinant Monoclonal Antibody
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中文名稱：NDUFAF1 Recombinant Monoclonal Antibody
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貨號：CSB-RA219742A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: Jurkat whole cell lysate(30μg), Raji whole cell lysate(30μg), HepG2 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), Hela whole cell lysate(30μg)
All lanes: NDUFAF1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 38 kDa
Observed band size: 33 kDa
Exposure time：1min -
IHC image of CSB-RA219742A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Overlay Peak curve showing jurkat cells stained with CSB-RA219742A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：
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別名：CGI 65 antibody; CGI65 antibody; CIA30 antibody; CIA30_HUMAN antibody; Complex I intermediate associated protein 30 antibody; Complex I intermediate associated protein 30 mitochondrial antibody; Complex I intermediate-associated protein 30; mitochondrial antibody; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 antibody; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 antibody; NADH:ubiquinone oxidoreductase complex assembly factor 1 antibody; NDUFAF 1 antibody; Ndufaf1 antibody
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide derived from human NDUFAF1
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：2D4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, IHC, FC
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推薦稀釋比：
Application Recommended Dilution IHC 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Chaperone protein involved in early stages of the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
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基因功能參考文獻(xiàn)：
- a case of leukodystrophy associated with mitochondrial complex I deficiency due to a novel mutation in the NDUFAF1 gene. PMID: 24963768
- oncogenic K-Ras is able to induce significant alterations in mitochondrial protein expression, and identified NDUFAF1 as an important molecule whose low expression contributes to mitochondrial dysfunction induced by K-Ras. PMID: 25714130
- NDUFAF1 is an important protein for the assembly/stability of complex I (NADH:ubiquinone oxidoreductase). PMID: 16218961
- Study shows a mitochondrial function for Ecsit in the assembly of mitochondrial complex I, an N-terminal targeting signal directs Ecsit to mitochondria, where it interacts with assembly chaperone NDUFAF1. PMID: 17344420
- the involvement of NDUFAF1 in the assembly process could be indirect rather than direct via the binding to assembly intermediates PMID: 17383918
- CIA30 is a crucial component in the early assembly of complex I and mutations in its gene can cause mitochondrial disease PMID: 17557076
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亞細(xì)胞定位：Mitochondrion. Mitochondrion matrix.
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蛋白家族：CIA30 family
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組織特異性：Ubiquitous.
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數(shù)據(jù)庫鏈接：
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