MMP9 Recombinant Monoclonal Antibody

說明書

中文名稱：

MMP9 Recombinant Monoclonal Antibody
貨號：

CSB-RA014679MA1HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA014679MA1HU diluted at 1:150 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA014679MA1HU MMP9重組單克隆抗體是針對基質金屬蛋白酶9（MMP9）研發(fā)的高特異性科研試劑。該抗體靶向的MMP9蛋白屬于鋅依賴性內肽酶家族，在細胞外基質重塑、炎癥反應及腫瘤微環(huán)境調控等生理病理過程中發(fā)揮重要作用。經嚴格驗證，本產品在ELISA實驗中展現(xiàn)出優(yōu)異的抗原結合能力，免疫組化（IHC）應用時推薦使用1:50-1:200的稀釋范圍，能夠在石蠟包埋組織切片中精準定位MMP9蛋白的表達分布，實驗結果顯示清晰的陽性信號與低背景干擾。抗體采用重組技術制備，具有批次間高度一致性和穩(wěn)定性，可特異性識別天然構象及變性狀態(tài)的MMP9蛋白。適用于體外培養(yǎng)細胞模型、病理組織樣本的蛋白表達分析，為腫瘤侵襲轉移機制研究、慢性炎癥性疾病探索以及組織修復相關科研項目提供可靠工具。本產品經多物種交叉反應驗證，建議配合陽性和陰性對照實驗以優(yōu)化檢測條件。
Uniprot No.：

P14780
基因名：

MMP9
別名：

82 kDa matrix metalloproteinase-9 antibody; 92 kDa gelatinase antibody; 92 kDa type IV collagenase antibody; CLG 4B antibody; CLG4B antibody; Collagenase Type 4 beta antibody; Collagenase type IV 92 KD antibody; EC 3.4.24.35 antibody; Gelatinase 92 KD antibody; Gelatinase B antibody; Gelatinase beta antibody; GelatinaseB antibody; GELB antibody; Macrophage gelatinase antibody; MANDP2 antibody; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody; Matrix Metalloproteinase 9 antibody; MMP 9 antibody; MMP-9 antibody; MMP9 antibody; MMP9_HUMAN antibody; Type V collagenase antibody
反應種屬：

Human
免疫原：

Recombinant Human MMP9 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號：

9B2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
基因功能參考文獻：
1. Cyclic peptides 1, 2, 46 also displayed potential interaction with active residues of MMP9; thus, these cyclic peptides can serve as potential drug candidates to block MMP9 activity and future studies are warranted to confirm their efficacy. PMID: 30226602
2. Our findings indicate that monitoring serum MMP-9 and detection of histological MMP-9 could help identify TNBC patients who will respond to NAC and will display varying risks of disease relapse. MMP-9 may serve as a predictive and prognostic biomarker for tailoring and modifying the NAC strategy for TNBC. PMID: 30241470
3. significantly increased in the serum of type 2 diabetes mellitus patients with diabetic retinopathy PMID: 29752166
4. Study evaluated for the first time the plasma levels and the diagnostic usefulness of VEGF, MMP-9, and TIMP-1 in cervical cancer (CC), not only independently but especially in combination with established cervical tumor markers. All tested parameters showed statistical significance when compared their concentrations in patients with CC to healthy women. PMID: 30037277
5. In monocytes, incubation with psoriasis' sera increased the production of MMP-9 and TIMP-1 in comparison with both baseline and monocytes incubated with non-psoriatic sera PMID: 29979898
6. High mmp9 expression is associated with Endometrial adenocarcinoma. PMID: 30139240
7. There was studied the level of matrix metalloproteinase-9 (MMP-9) in plasma of bone marrow aspirates in 87 patients: 39 with acute myeloid leukemia (AML) and 48 myelodysplastic syndrome (MDS). It has been found out an association of the level of MMP-9 in plasma of bone marrow aspirates in patients with AML and MDS with a volume of leukemic mass. PMID: 30444586
8. This study provides the first evidence for the involvement of polymorphisms within MMP9 and the severity of childhood trauma in antipsychotic treatment response PMID: 29448178
9. HOXD-AS1 was upregulated in non-small cell lung carcinoma tissues and cell lines and promoted NSCLC cell proliferation, migration invasion via sponging miR-133b and regulating MMP9. PMID: 29958139
10. rs3918241, rs2250889, rs17576 and rs17577 polymorphisms of MMP-9 are not associated with altered risk of urinary cancer. PMID: 30249503
11. PARP-1, via manipulating the binding of NF-kB/AP-1 at the MMP-9 promoter, regulates MMP-9 expression, which helps maintain mitochondrial homeostasis. PMID: 28478229
12. The results suggest that the MMP9 gene may affect genetic predisposition to tick-borne encephalitis in a Russian population. PMID: 29496490
13. MMP-9 was targeted by hsa-miR-494, which inhibited OS. PMID: 29068478
14. In placenta, a significant positive correlation between MMP-9 and gestational age was noted in the first trimester. When gestational age was controlled for, partial correlation revealed a significant positive correlation between TNF-alpha and MMP-9 only in the second trimester. PMID: 28820024
15. Melatonin inhibits epithelialtomesenchymal transition in gastric cancer cells via attenuation of IL1beta/NFkappaB/MMP2/MMP9 signaling. PMID: 30066836
16. Data indicate that CD147 promotes breast cancer cell proliferation, metastasis, and invasion by modulating matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor (VEGF) expression. PMID: 29901696
17. immune system process is indispensable in the progression of disease in colon, and identifies that IL-8 and MMP-9 play potential critical roles for the progression. PMID: 30074183
18. The demethylation of the MMP-9 gene promoter region may be involved in the occurrence and development of diabetic nephropathy by regulating the expression of MMP-9 protein in serum. PMID: 29952417
19. Patients carrying the variant T allele of the MMP9 rs3918242 polymorphism exhibited significantly higher infarct volumes than those with the major CC genotype. PMID: 30257242
20. Increased matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio in smokers with airway hyperresponsiveness is associated with accelerated lung function decline. PMID: 29692608
21. MMP-9 and TIMP-1 levels are correlated with age, pathological grade, tumor size and lymph node metastasis of breast cancer patients. PMID: 30010105
22. When ANXA5 expression increased, cell proliferation was inhibited by regulating the expression of bcl-2 and bax while cell metastasis was suppressed by regulating E-cadherin and MMP-9 expression. PMID: 30010106
23. IL-33 can regulate deposition of ECM and promote the process of pulmonary fibrosis by inducing the imbalance between MMP-9 and TIMP-1. PMID: 29417309
24. Results show that MMP9 specifically binds to H3K27me1 nucleosomes allowing the cleavage of H3N-terminal tail. PMID: 29807539
25. Study shows that rs1056629 polymorphism in the 3'UTR of MMP9 disrupts its binding to miR-491 in pressure ulcer (PU)patients after hip fracture. mRNA and protein expression levels are high in PU specimens. PMID: 29620239
26. No association between the carriage of studied MMP9 polymorphisms and the predisposition to preeclampsia was found. PMID: 29670668
27. the logistic regression analysis, none of the genes was associated with AOB (anterior open bite). In conclusion, the polymorphism rs17576 (glutamine for arginine substitution) in MMP9 was a protective factor for AOB PMID: 29297546
28. Results provide evidence that MMP9 plays critical roles in mesenchymal stem cells high suppressive potency, which also involves the capacity to induce and integrated multiple immunoregulatory mechanisms. PMID: 28408751
29. piperine reduced the expression of pAkt, MMP9 and pmTOR. Together, these data indicated that piperine may serve as a promising novel therapeutic agent to better overcome prostate cancer metastasis. PMID: 29488612
30. Our study demonstrated that both positive expression status in tumor tissue and expression levels of MMP-9 are significantly elevated in BC patients and correlate with disease progression. Thus, MMP-9 can serve as a biomarker to determine the degree of BC malignancy. PMID: 29578150
31. Our findings indicate that overexpression of MMP-9 rescued insulin survival signaling in vitro and in early stages in the 5XFAD model of AD. PMID: 28386117
32. Review/Meta-analysis: Matrix metalloproteinase-9 has high predictive value for hemorrhagic transformation after acute ischemic stroke. PMID: 29598905
33. The expression of MMP-9 is associated with clinical outcome in chondrosarcoma. PMID: 29316907
34. MMP-9 levels in the tumor tissue extracts had also increased significantly PMID: 29520667
35. MMP-9 -1562 C/T polymorphism might be related to the digestive cancer susceptibility [meta-analysis] PMID: 29890308
36. MMP-9 protein overexpression was found in prostate cancers, low expression in any of the normal tissues or in benign prostatic tissue PMID: 27355218
37. Hypoxia promotes retinoblastoma cell line HXO-RB44 invasion by activating HIF-1alpha/MMP9 signaling pathway. PMID: 29243776
38. Authors conclude that ADAM8 promotes early metastatic processes such as transendothelial migration by upregulation of MMP-9 and shedding of PSGL-1 from breast cancer cells. PMID: 28986926
39. High expression of MMP-9 and TLR4 in patients with COPD may promote inflammatory cell infiltration, induce proliferation of smooth muscle cells, degrade extracellular matrix, and play an important role in lung revascularization. PMID: 28537664
40. The data obtained from this study show that mild hypothermia therapy down regulated the expression of MMP-9 mRNA, the MMP-9 protein level and increased the FOUR score and GCS in high-risk TBI patients within 72 h. PMID: 28966304
41. High MMP9 expression is associated with cervical cancer. PMID: 30052166
42. MMP-9 overexpression and activation are important events occurring during oral squamous cell carcinoma progression/invasion and that this overexpression/activation is regulated by c-Myc, active MMP-2 and inactive GSK3beta mediated pathways. PMID: 29134466
43. Plasma concentration of MMP9 demonstrated no associations with stroke occurrence in a West African cohort. PMID: 29074065
44. High mmp9 expression is associated with ovarian cancer cell migration and invasion. PMID: 28677781
45. no significant difference was detected in the expression level of individual MMP9 and TIMP1 genes in SCZ patients versus healthy controls either in total numbers of subject or in sex based subgroups PMID: 28578515
46. Data suggest that MIR129 or MIR335 overexpression in keratinocytes inhibits MMP9 promoter activity and protein expression by targeting SP1; inhibition of these microRNAs has the opposite effect. These mechanisms may be involved in regulation of wound healing. (MIR = microRNA; MMP9 = matrix metalloproteinase-9; SP1 = transcription factor-SP1) PMID: 29748291
47. Significant association observed for MMP-9 (-1562C/T) (OR 1.27; 95% CI 1.06 to 1.53) gene polymorphism and risk of ischemic stroke. [meta-analysis] PMID: 29906531
48. MMP-9 activity increases postpartum. Women experiencing uncomplicated vaginal delivery demonstrate higher postpartum MMP-9 activity and are more likely to have recovered their vaginal angle. PMID: 29155140
49. Results show that MMP9 degrades MMRN2 allowing endothelial cells migration and sprouting angiogenesis. PMID: 28435016
50. The findings suggest that AEG-1 promotes gastric cancer metastasis through upregulation of eIF4E-mediated MMP-9 and Twist. PMID: 28661037
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相關疾病：

Intervertebral disc disease (IDD); Metaphyseal anadysplasia 2 (MANDP2)
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
組織特異性：

Detected in neutrophils (at protein level). Produced by normal alveolar macrophages and granulocytes.
數(shù)據(jù)庫鏈接：

HGNC: 7176

OMIM: 120361

KEGG: hsa:4318

STRING: 9606.ENSP00000361405

UniGene: Hs.297413

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