LRP8 Recombinant Monoclonal Antibody

說明書

中文名稱：

LRP8 Recombinant Monoclonal Antibody
貨號：

CSB-RA825731A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of LDL receptor related protein 8 expression in HepG2 cells using LDL receptor related protein 8 antibody. Green, isotype control; red, LDL receptor related protein 8.

Immunocytochemical staining of HepG2 cells with LDL receptor related protein 8 antibody. Nuclei were stained blue with DAPI; LDL receptor related protein 8 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using LDL receptor related protein 8 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LDL receptor related protein 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q14114
基因名：

LRP8
別名：

LRP8; LDL Receptor Related Protein 8; APOER2; LRP-8; HSZ75190; MCI1; Low Density Lipoprotein Receptor-Related Protein 8, Apolipoprotein E Receptor; Low-Density Lipoprotein Receptor-Related Protein 8; Apolipoprotein E Receptor 2; ApoE Receptor 2
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human LRP8 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3H5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor. Not required for endocytic uptake of SEPP1 in the kidney which is mediated by LRP2. Together with its ligand, apolipoprotein E (apoE), may indirectly play a role in the suppression of the innate immune response by controlling the survival of myeloid-derived suppressor cells.
基因功能參考文獻：
1. ABGL4, LRP8 and PCSK9 polymorphisms and gene interactions increase cardiometabolic risk. PMID: 27853278
2. This study elucidates that the contracted-open conformation of ligand-bound ApoER2 at neutral pH resembles the contracted-closed conformation of ligand-unbound low-density lipoprotein receptor at acidic pH in a manner suggestive of being primed for ligand release even prior to internalization. PMID: 28446613
3. LRP8 is a risk gene for psychosis. PMID: 26637325
4. Results show that none of the SNPs were significantly associated with myocardial infarction (MI). Interestingly, haplotype based association analysis showed TG and CG of rs10788952 and rs7546246 significantly associated with MI and in particular, haplotype TG was positively correlated with the risk of MI, as this increased the LDL and total cholesterol level in MI patients in south Indians. PMID: 29032149
5. These results reveal an association between ApoER2 isoform expression and Alzheimer's disease. PMID: 26902204
6. ApoER2 contributes cooperatively with endothelial cell protein C receptor and protease activated receptor 1 to APC-initiated endothelial antiapoptotic and barrier protective signaling. PMID: 26800564
7. two SNPs (rs3737984 and rs2297660) in ApoER2 gene had significant association with dyslipidemia in Thai ethnic PMID: 26817215
8. A novel TACGC risk haplotype in the LRP8 gene that is present in patients with CAD and MI but not in normal controls. PMID: 24867879
9. results of this study demonstrated the presence of reelin, its receptors VLDLR and ApoER2 as well as Dab1 in the ENS and might indicate a novel role of the reelin system in regulating neuronal plasticity and pre-synaptic functions in the ENS. PMID: 24844606
10. Data suggest that PS1/gamma-secretase-dependent processing of the reelin receptor ApoER2 inhibits reelin expression and may regulate its signaling. PMID: 24344333
11. By incorporating the information from bioinformatics and RNA expression analyses, we identified at least two of the most promising risk genes for alcohol dependence: APOER2 and UBAP2 PMID: 23739027
12. TCCGC haplotype at the 3'-terminal block of the LRP8 gene confers a protective role in the development of familial and early-onset coronary artery disease and/or myocardial infarction. PMID: 23524007
13. In a Chinese unrelated Han population, variants within the LRP8 gene do not convey the risk of developing Parkinson's disease. PMID: 22889673
14. results identify LRP8 as a novel positive factor of canonical Wnt signaling pathway PMID: 22589174
15. genetic variant R952Q of LRP8 is associated with increased plasma TG levels in patients who are overweight and have premature CAD/MI and history of smoking. PMID: 22404453
16. ApoEr2 plays important roles in structure and function of CNS synapses and dendritic spines PMID: 21347244
17. Deficient sLRP-amyloid-beta binding might precede and correlate later in disease with an increase in the tau/Abeta42 CSF ratio and global cognitive decline in mild cognitive impairment individuals converting into Alzheimer's disease. PMID: 21157031
18. LRP8 gene polymorphisms influence plasma cholesterol levels as well as size and composition of LDL particles PMID: 21316997
19. the expression of ApoER2 may serve as a trait marker for major depressive disorder. PMID: 20493228
20. There were seven polymorphisms in apoE receptor 2 in Japanese sporadic Alzheimer disease patients, but no association of these polymorphisms with Alzheimers. PMID: 20208369
21. Differential functions of ApoER2 and very low density lipoprotein receptor in Reelin signaling depend on differential sorting of the receptors. PMID: 19948739
22. ApoEr2 regulates cell movement, and both X11alpha and Reelin enhance this effect. PMID: 19720620
23. transmembrane domain and PXXP motif excludes it from carrying out clathrin-mediated endocytosis PMID: 12621059
24. Data show that the apolipoprotein E receptor 2 binding domain of apolipoprotein E is in the 1-165 amino terminal region, whereas the carboxy terminal 230-299 region of apoE is required for efficient initial association with phospholipids. PMID: 12950167
25. Complete molecular structure examined by nuclear magnetic resonance. PMID: 16034672
26. ApoEr2 can form a multiprotein complex with NMDA receptor subunits and PSD95 PMID: 16332682
27. Reelin signals by binding to two transmembrane receptors, apolipoprotein E receptor 2 (Apoer2) and very-low-density lipoprotein receptor. PMID: 16481437
28. In conclusion, results from the two independent samples of black women provide consistent evidence that SNP rs2297660 in LRP8 is associated with fetal growth. PMID: 16642433
29. The effect of Dab1 on APP and apoEr2 processing in transfected cells and primary neurons is reported. PMID: 16951405
30. The presence of three splice variants of ApoER2 on platelets was confirmed by immuno-blotting, with ApoER2Delta4-5 being the most abundantly expressed splice variant. PMID: 17470198
31. However, this polymorphism increased the risk of AD conferred by the MAPK8IP1 G allele. PMID: 17614163
32. A nonconservative substitution, R952Q, in LRP8 was significantly associated with susceptibility to premature CAD and/or MI by use of both population-based and family-based designs. PMID: 17847002
33. Fyn, due in part to its effects on Dab1, regulates the phosphorylation, trafficking, and processing of APP and apoEr2. PMID: 18089558
34. protein C and APC may directly promote cell signaling in other cells by binding to ApoER2 and/or GPIbalpha PMID: 18489431
35. Study showed no evidence for association of genetic variants in the LRP8 gene with familial and sporadic myocardial infarction. PMID: 18592168
36. ligation of ApoER2 by APC signals via Dab1 phosphorylation and subsequent activation of PI3K and Akt and inactivation of GSK3beta, thereby contributing to APC's beneficial effects on cells. PMID: 19116273
37. Our data suggest that LRP8 R952Q variant may have an additive effect to APOE epsilon2/epsilon3/epsilon4 genotype in determining ApoE concentrations and risk of MI in an Italian population PMID: 19439088
38. apolipoprotein E receptor 2 (ApoER2, LRP8), a member of the LDL receptor family, is a platelet receptor for FXI. PMID: 19661487
39. Sequential proteolytic processing of murine ApoER2 results in the release of its intracellular domain by the protease gamma-secretase. The prior cleavage of its extracellular domain is determined by the glycosylation state of the receptor. PMID: 12871934
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相關疾病：

Myocardial infarction 1 (MCI1)
亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Secreted.
蛋白家族：

LDLR family
組織特異性：

Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
數據庫鏈接：

HGNC: 6700

OMIM: 602600

KEGG: hsa:7804

STRING: 9606.ENSP00000303634

UniGene: Hs.280387

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