Immunocytochemical staining of HAP-1 cells with Leucine aminopeptidase 3 antibody. Nuclei were stained blue with DAPI; Leucine aminopeptidase 3 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Leucine aminopeptidase 3 expression in HAP-1 cells using Leucine aminopeptidase 3 antibody. Green, isotype control; red, Leucine aminopeptidase 3.
Western blotting analysis using Leucine aminopeptidase 3 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Leucine aminopeptidase 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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貨期:
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用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Cytolosic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides. The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme. For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates. Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status.
基因功能參考文獻:
LAP3 might be a new prognostic factor and be close correlation with glioma cell growth, migration, invasion. PMID: 25453285
our results indicated that up-regulated expression of LAP3 might contribute to the proliferation and metastasis of hepatocellular carcinoma PMID: 25120751
Overexpression of leucine aminopeptidase 3 contributes to malignant development of human esophageal squamous cell carcinoma. PMID: 24477662
results suggest that Lys528 is important for maximal activity of adipocyte-derived LAP by maintaining the appropriate structure of the substrate pocket of the enzyme PMID: 16513116