ILDR2 Recombinant Monoclonal Antibody
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中文名稱：ILDR2 Recombinant Monoclonal Antibody
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貨號：CSB-RA751251MA1HU
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規(guī)格：￥1320
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圖片：
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Immunofluorescence?staining?of?Hela?cell?with?CSB-RA751251MA1HU?at?1:30,?counter-stained?with?DAPI.?The?cells?were?fixed?in?4%?formaldehyde?and?blocked?in?10%?normal?Goat?Serum.?The?cells?were?then?incubated?with?the?antibody?overnight?at?4C.?The?secondary?antibody?was?FITC-conjugated?AffiniPure?Goat?Anti-Human?IgG(H+L). -
Immunofluorescence?staining?of U251?cell?with?CSB-RA751251MA1HU?at?1:30,?counter-stained?with?DAPI.?The?cells?were?fixed?in?4%?formaldehyde?and?blocked?in?10%?normal?Goat?Serum.?The?cells?were?then?incubated?with?the?antibody?overnight?at?4C.?The?secondary?antibody?was?FITC-conjugated?AffiniPure?Goat?Anti-Human?IgG(H+L). -
Overlay Peak curve showing Jurkat cells stained with CSB-RA751251MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100.Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA751251MA1HU ILDR2重組單克隆抗體是經(jīng)ELISA、免疫熒光（IF）和流式細胞術(shù)（FC）嚴格驗證的高特異性科研工具。該抗體靶向ILDR2蛋白，該分子作為免疫球蛋白超家族成員，在細胞間連接復合體形成及上皮極性建立中發(fā)揮重要作用，與組織屏障功能調(diào)控密切相關(guān)。實驗數(shù)據(jù)顯示，該抗體在IF應(yīng)用中能清晰呈現(xiàn)細胞膜或特定亞細胞結(jié)構(gòu)的定位信號，推薦使用稀釋比例為1:50-1:200；在FC檢測中可有效識別天然構(gòu)象的靶蛋白，推薦同等稀釋范圍。經(jīng)ELISA驗證其具備優(yōu)異的目標蛋白結(jié)合能力，適用于體外蛋白互作分析。本產(chǎn)品可廣泛應(yīng)用于細胞連接機制研究、上皮組織發(fā)育模型構(gòu)建，以及相關(guān)信號通路的功能解析等基礎(chǔ)研究領(lǐng)域，為探索ILDR2在細胞極性調(diào)控和生理病理過程中的分子機制提供可靠支持。
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Uniprot No.：
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基因名：
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別名：ILDR2; C1orf32Immunoglobulin-like domain-containing receptor 2
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反應(yīng)種屬：Human
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免疫原：Recombinant Human ILDR2 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：hIgG1
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純化方式：Affinity-chromatography
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克隆號：13G7
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, IF, FC
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推薦稀釋比：
Application Recommended Dilution IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
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靶點詳情
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功能：May be involved in lipid homeostasis and ER stress pathways.
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基因功能參考文獻：
- We demonstrated that ZNF70 interacts with ZFP64 and activates HES1 transcription by binding to the HES1 promoter. In addition, HES1 gene expression is increased in ILDR2-knockdown HepG2 cells, in which ZNF70 is translocated from the cytoplasm to the nucleus, suggesting that ZNF70 migration to the nucleus after dissociating from the ILDR2-ZNF70 complex activates HES1 transcription. These results support a novel link betwee PMID: 27353377
- Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
- Observational study of gene-disease association. (HuGE Navigator) PMID: 19536175
- Observational study of gene-disease association. (HuGE Navigator) PMID: 19064610
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亞細胞定位：Endoplasmic reticulum membrane; Single-pass type I membrane protein.
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蛋白家族：Immunoglobulin superfamily, LISCH7 family
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數(shù)據(jù)庫鏈接：
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