HGD Recombinant Monoclonal Antibody
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中文名稱：HGD重組抗體
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貨號：CSB-RA695552A0HU
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規(guī)格：￥1320
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圖片：
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IHC image of CSB-RA695552A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA695552A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA695552A0HU HGD重組單克隆抗體是針對人類尿黑酸1,2-雙加氧酶（Homogentisate 1,2-dioxygenase）研發(fā)的高特異性檢測工具，該酶是酪氨酸代謝途徑中的關(guān)鍵催化酶，其功能缺陷與遺傳性尿黑酸癥及相關(guān)骨關(guān)節(jié)病變密切相關(guān)。本抗體經(jīng)ELISA和免疫組化（IHC）嚴格驗證，在1:50-1:200稀釋范圍內(nèi)可清晰顯示組織樣本中HGD蛋白的定位分布，實驗數(shù)據(jù)顯示其在多種人類組織切片中呈現(xiàn)特異性染色模式，檢測結(jié)果與靶蛋白預(yù)期表達特征高度吻合，且不同批次產(chǎn)品具有良好穩(wěn)定性。適用于探究HGD在代謝性疾病發(fā)生發(fā)展中的作用機制，特別是在構(gòu)建酪氨酸代謝異常模型、骨關(guān)節(jié)退行性病變研究及病理樣本分析等領(lǐng)域具有重要應(yīng)用價值。該抗體為科研人員研究HGD蛋白表達調(diào)控及其在遺傳代謝缺陷中的病理生理意義提供了可靠工具。
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Uniprot No.：
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基因名：HGD
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別名：Homogentisate 1,2-dioxygenase (EC 1.13.11.5) (Homogentisate oxygenase) (Homogentisic acid oxidase) (Homogentisicase), HGD, HGO
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide derived from human HGD
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：20D12
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, IHC
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推薦稀釋比：
Application Recommended Dilution IHC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
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靶點詳情
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基因功能參考文獻：
- Twelve novel HGD gene variants have been identified in 99 alkaptonuria patients affecting the bones. PMID: 25804398
- A novel alkaptonuria mutation, c.87 + 1G > A, shows a significant founder effect and high prevalence in a nomadic Indian population. PMID: 24575791
- study described two novel HGD mutations in a Chinese alkaptonuria family, the splicing mutation of IVS7 1G>C, a donor splice site of exon 7, and a missense mutation of F329C in exon 12 PMID: 23353776
- Report mutations of the HGD gene in Jordanian alkaptonuria patients. PMID: 21437689
- The observed increase of HGD expression in Alkaptonuria cells is probably due to a compensatory mechanism to overcome the almost null catalytic activity of the deficient enzyme PMID: 22105303
- An update on molecular genetics of Alkaptonuria (AKU).(review) PMID: 21720873
- Familiar ochronotic arthropathy is caused by a gene mutation of the homogentisic acid 1,2-dioxygenase (HGD) gene traced three hundred years in a Hungarian family. PMID: 20462779
- A comprehensive mutation analysis of 93 patients enrolled in this study, as well as an extensive update of all previously published HGD mutations associated with alkaptonuria, is reported. PMID: 19862842
- Turkish mutation shares an homogentisate 1,2-dioxygenase haplotype with the mutation found in Finland, Slovakia and India, suggesting that R58fs is an old alkaptonuria mutation that probably originated in central Asia PMID: 12872836
- Four different mutations of the HGD gene were found in alkaptonuria and ochronotic arthropathy diagnoses. PMID: 16085442
- A single nucleotide deletion located in exon 3 resulted in a frameshift mutation in HGD gene in family with alkaptonuria. PMID: 18945288
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相關(guān)疾?。?/div>Alkaptonuria (AKU)蛋白家族：Homogentisate dioxygenase family組織特異性：Highest expression in the prostate, small intestine, colon, kidney and liver.數(shù)據(jù)庫鏈接：
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