ELP4 Recombinant Monoclonal Antibody
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中文名稱：ELP4 Recombinant Monoclonal Antibody
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貨號：CSB-RA264351A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: A431 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), A549 whole cell lysate(30μg), A2780 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Rat brain tissue lysate(30μg)
All lanes:Elongator complex protein 4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time：1min -
Immunofluorescence staining of MCF-7 cell with CSB-RA264351A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing Hela cells stained with CSB-RA264351A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：ELP4
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別名：C11orf19 antibody; dJ68P15A.1 antibody; elongation protein 4 homolog (S. cerevisiae) antibody; Elongation protein 4 homolog antibody; Elongator complex protein 4 antibody; ELP4 antibody; ELP4_HUMAN antibody; FLJ20498 antibody; hELP4 antibody; PAX6 neighbor gene protein antibody; PAX6NEB antibody; PAXNEB antibody
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反應種屬：Human, Mouse, Rat
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免疫原：A synthesized peptide from human ELP4 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：12F8
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Component of the RNA polymerase II elongator complex, a multiprotein complex associated with the RNA polymerase II (Pol II) holoenzyme, and which is involved in transcriptional elongation. The elongator complex catalyzes formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.
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基因功能參考文獻：
- Our results suggest that ELP4 deletions are highly likely to be pathogenic, predisposing to a range of neurodevelopmental phenotypes from ASD to language impairment and epilepsy. PMID: 26010655
- Results show no difference in BDNF and ELP4 genotype frequencies between benign epilepsy with centrotemporal spikes patients and controls; haplotype analysis also revealed no statistical difference PMID: 25301525
- No association of SNPs in the ELP4 gene was detected in rolandic epilepsy patients. PMID: 24995671
- Data suggest that a non-coding mutation in ELP4 impairs brain-specific Elongator-mediated interaction of genes implicated in brain development, resulting in susceptibility to seizures and neurodevelopmental disorders. PMID: 19172991
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相關疾病：Aniridia 2 (AN2)
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亞細胞定位：Cytoplasm. Nucleus.
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蛋白家族：ELP4 family
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組織特異性：Widely expressed.
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數據庫鏈接：
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