EHMT2 Recombinant Monoclonal Antibody

說明書

中文名稱：

EHMT2 Recombinant Monoclonal Antibody
貨號：

CSB-RA977737A0HU
規格：

￥1320
圖片：
Immunocytochemical staining of HepG2 cells with EHMT2 antibody. Nuclei were stained blue with DAPI; EHMT2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of EHMT2 expression in HepG2 cells using EHMT2 antibody. Green, isotype control; red, EHMT2.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using EHMT2 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded mouse brain using EHMT2 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using EHMT2 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EHMT2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q96KQ7
基因名：

EHMT2
別名：

Euchromatic Histone Lysine Methyltransferase 2; KMT1C; G9A; C6orf30; BAT8; Euchromatic Histone-Lysine N-Methyltransferase 2; Histone-Lysine N-Methyltransferase EHMT2; Histone H3-K9 Methyltransferase 3; HLA-B Associated Transcript 8; Lysine N-Methyltransferase 1C; H3-K9-HMTase 3; Em:AF134726.3; NG36/G9a; NG36; Histone-Lysine N-Methyltransferase, H3 Lysine-9 Specific 3; Chromosome 6 Open Reading Frame 30; Ankyrin Repeat-Containing Protein; G9A Histone Methyltransferase; HLA-B-Associated Transcript 8; EC 2.1.1.367; Protein G9a; EC 2.1.1.-; GAT8
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human EHMT2 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

20B10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000
IHC	1:100-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
基因功能參考文獻：
1. Our results demonstrate of the central role of G9a HMT in the promotion of endothelial cells proliferation, and suggest that endothelial G9a HMT may be a target in the treatment of vascular proliferative disorders and tumor neovascularization. PMID: 29113759
2. unveil E4BP4 as a critical transcriptional modulator repressing RASSF8 expression through histone methyltransferases, G9a and SUV39H1. PMID: 29467226
3. Results establish a direct functional link between G9a and MMP-9 in the context of pro-osteoclastogenic transcriptional programs. The model suggests that G9a mediated H3K27me1 serves as an essential mark for MMP-9 recruitment and proteolytic activity at genes encoding positive regulators of osteoclast differentiation, thus keeping them active. PMID: 29807539
4. The study identified that decreased expression of SATB2 was correlated with tumor progression and poor prognosis in non-small cell lung cancer (NSCLC) patients. Furthermore, SATB2 suppressed lung cancer cell invasion and metastasis and regulated the expression of EMT-related proteins and histone methylation by G9a. PMID: 28667416
5. G9a plays a role as an epigenetic mediator of hypoxic response in breast cancer PMID: 28630300
6. Identified the tumor suppressor RARRES3 as a critical target of G9a. Epigenetic silencing of RARRES3 contributed to the tumor-promoting function of G9a PMID: 28532996
7. regulatory pathway based on casein kinase 2-G9a-RPA permits homologous recombination in cancer cells PMID: 28698370
8. these findings reveal a role for REST-associated G9a and histone H3K9 methylation in the repression of USP37 expression in medulloblastoma. Reactivation of USP37 by G9a inhibition has the potential for therapeutic applications in REST-expressing medulloblastomas PMID: 28483947
9. Regulated methylation and phosphorylation serve as a switch controlling G9a and GLP coactivator function, suggesting that this mechanism may be a general paradigm for directing specific transcription factor and coregulator actions on different genes. PMID: 28615290
10. interleukin-8 is a downstream effector of G9a to increase gemcitabine resistance in pancreatic cancer. Overexpression of G9a correlated with poor survival and early recurrence in pancreatic cancer patients. PMID: 27531902
11. Taken together, our findings provide evidence that G9a protects head and neck squamous cell carcinomas (HNSCC)cells against chemotherapy by increasing the synthesis of GSH, and imply G9a as a promising target for overcoming cisplatin resistance in HNSCC PMID: 28265008
12. In this study, EHMT2 (a histone methyltransferase) was determined to be significantly overexpressed in breast cancer tissues and in Oncomine data. In addition, knockdown of EHMT2 reduced cell migration and invasion. PMID: 29337058
13. Knockdown of G9a increased the sensitivity of cells to radiation treatment and sensitized cells to DNA damage agents through PP2A-RPA axis. PMID: 28351524
14. EHMT2 can directly repress Beclin-1 and the inhibition of EHMT2 may be a useful therapeutic approach for cancer prevention by activating autophagy PMID: 27174920
15. Loss of miR-1 and gene copy number gain of G9a contributed to its frequent upregulation in liver cancer, which epigenetically silenced the expression of tumor suppressor RARRES3, and ultimately contributed to hepatocellular carcinoma (HCC) cell proliferation and migration. PMID: 28532996
16. G9a promotes breast cancer by regulating iron metabolism through the repression of ferroxidase hephaestin. PMID: 28819251
17. study uncovers a novel mechanism of G9A promoting tumor cell growth and invasion by silencing CASP1, and implies that G9A may serve as a therapeutic target in treating Non-small-cell lung cancer. PMID: 28383547
18. G9a inhibition impairs anchorage-dependent and -independent cell growth in hepatocellular carcinoma cells PMID: 28423509
19. A histone H3K9-like mimic within LIG1 is methylated by G9a and GLP and avidly binds UHRF1. Interaction with methylated LIG1 promotes the recruitment of UHRF1 to DNA replication sites and is required for DNA methylation maintenance. PMID: 28803780
20. G9a is an important regulator in placental diseases caused by defective vascular maturation. PMID: 28455378
21. Low G9a expression is associated with lung and colonic cancer. PMID: 27452519
22. G9A overexpression is associated with peritoneal fibrosis. PMID: 28278257
23. G9a inhibition potentiates the anti-tumour activity of DNA double-strand break inducing agents by impairing DNA repair independent of p53 status. PMID: 27431310
24. Inhibition of EZH2 (by GSK-343 or EPZ-6438) or inhibition of EHMT2 (by UNC-0638) in the Th17 primary cell model of HIV latency or resting memory T cells isolated from HIV-1-infected patients receiving highly active antiretroviral therapy, was sufficient to induce the reactivation of latent proviruses. PMID: 28246360
25. we find an estrogen receptor-independent synthetic lethal interaction between a GATA3 frameshift mutant with an extended C-terminus and the histone methyltransferases G9A and GLP, indicating perturbed epigenetic regulation PMID: 27588951
26. High expression of G9a and H3K9me2 in glioma tissue samples was associated with the WHO grade, which indicated that G9a and H3K9me2 may promote generation and development of glioma. BIX01294 may be a potential novel therapeutic agent in the treatment of glioma. PMID: 27748874
27. Depletion of G9a inhibits cell growth and induces cells apoptosis in gastric cancer. PMID: 27081761
28. G9a and GLP are required for stable maintenance of imprinted DNA methylation in embryonic stem cells. PMID: 27052169
29. The G9a exon 10(+) isoform is necessary for neuron differentiation and regulates the alternative splicing pattern of its own pre-mRNA, enhancing exon 10 inclusion. PMID: 26997278
30. G9a promotes H3K27 methylation of the E-cadherin promoter by upregulating PCL3 to increase PRC2 promoter recruitment and by downregulating the H3K27 demethylase KDM7A to silence E-cadherin gene PMID: 26688070
31. High G9a contributes to endometrial cancer progression. PMID: 25613390
32. LASP-1 associated with UHRF1, G9a, Snail1 and di- and tri-methylated histoneH3 in a CXCL12-dependent manner based on immunoprecipitation and proximity ligation assays PMID: 25982273
33. Along with the PHF20/MOF complex, G9a links the crosstalk between ERalpha methylation and histone acetylation that governs the epigenetic regulation of hormonal gene expression. PMID: 26960573
34. high expression in hepatocellular carcinoma associated with worse outcome PMID: 26765460
35. The present study aimed at examining the potential role of autophagy in the anti-proliferation effect of G9a inhibition on transitional cell carcinoma T24 and UMUC-3 cell lines in vitro. PMID: 26397365
36. Homocysteine Induces Collagen I Expression by Downregulating Histone Methyltransferase G9a PMID: 26192994
37. G9a has a dominant function in transcriptional repression of potassium channels and in acute-to-chronic pain transition. PMID: 26551542
38. KMT1c (G9a) is markedly up regulated after differentiation of monocytes into immature dendritic cells. PMID: 25843229
39. This study demonstrate that the increases in a restrictive epigenome seen in schizophrenia are sex dependent. Specifically,G9A were significantly increased in lymphocytes from men with schizophrenia. PMID: 25935252
40. Data indicate zinc finger proteins ZNF644 and WIZ as two core subunits in the histone-lysine N-methyltransferase G9a/GLP complex, and interact with the transcription activation domain of G9a and GLP. PMID: 25789554
41. data provide genetic and pharmacologic evidence that EHMT1 and EHMT2 are epigenetic regulators involved in gamma-globin repression and represent a novel therapeutic target for SCD. PMID: 26320100
42. G9a protein is essential for the induction of epithelial-to-mesenchymal transition and cancer stem cell-like properties in HNSCC PMID: 25749385
43. Down-regulation of G9a triggers DNA damage response and inhibits colorectal cancer cells proliferation. PMID: 25595900
44. The expression level of EHMT1 and EHMT2 inversely correlates with the type I interferon responsiveness in chronic myeloid leukemia cell lines. PMID: 25079219
45. G9a-dependent H3K9me2 repression on CD133 and Sox2 was one of the main switches of the self-renewal in glioma cancer stem cells. PMID: 24833465
46. The current knowledge on the mechanisms of action and function of EHMT2, with particular emphasis on their interplay in the regulation of chromatin states and biological processes. PMID: 25365549
47. MCM7 and G9a may serve as effective prognostic factors and could also be used as biomarkers for predicting various clinical outcomes of oesophageal squamous cell carcinoma PMID: 24805087
48. we defined a role for activated STAT3 and G9a histone methyltransferase in epigenetic silencing of miR-200c, which promotes the formation of breast CSCs defined by elevated cell surface levels of the leptin receptor PMID: 25840984
49. G9a contributes to multiple steps of ovarian cancer metastasis. PMID: 25115793
50. H3K9 methyltransferase G9a plays role in the apoptosis and autophagy in oral squamous cell carcinoma. PMID: 25634693
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亞細胞定位：

Nucleus. Chromosome. Note=Associates with euchromatic regions. Does not associate with heterochromatin.
蛋白家族：

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily
組織特異性：

Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
數據庫鏈接：

HGNC: 14129

OMIM: 604599

KEGG: hsa:10919

STRING: 9606.ENSP00000364687

UniGene: Hs.709218

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