DSC3 Recombinant Monoclonal Antibody

說明書

中文名稱：

DSC3 Recombinant Monoclonal Antibody
貨號(hào)：

CSB-RA830069A0HU
規(guī)格：

￥1320
圖片：
Flow cytometric analysis of Desmocollin 3 expression in HeLa cells using Desmocollin 3 antibody. Green, isotype control; red, Desmocollin 3.

Immunocytochemical staining of Hela cells with Desmocollin 3 antibody. Nuclei were stained blue with DAPI; Desmocollin 3 with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Immunohistochemistry was performed on paraffin-embedded human skin tissue using desmocollin 3 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded human esophageal carcinoma using desmocollin 3 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using Desmocollin 3 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Desmocollin 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

Uniprot No.：

Q14574
基因名：

DSC3
別名：

DSC3; Desmocollin 3; CDHF3; DSC1; DSC2; DSC4; DSC; Cadherin Family Member 3; Desmocollin-3; Desmocollin-4; HT-CP
反應(yīng)種屬：

Human
免疫原：

Recombinant Human DSC3 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

18B5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, FC, ICC, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000
IHC	1:100-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
Immunohistochemistry (IHC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.
基因功能參考文獻(xiàn)：
1. The Findings of this study imply early alteration of the substantia nigra in XDP mutation carriers prone to develop parkinsonism. PMID: 28094105
2. Data show that fetal pMSCs (mesenchymal stromal cells) expressing the highest levels of desmoglein 2, desmocollin 3 and plakophilin 2, followed by maternal pMSCs, while bmMSCs expressed the lowest levels. PMID: 28154962
3. Napsin-A, and Desmocollin-3 were sensitive and specific markers for the diagnosis of AC and SCC, respectively. Both markers allowed classification of 54/60 cases into either AC or SCC. PMID: 26710975
4. Suggest that Dsc3 can mediate FSH-induced ovarian cancer cell proliferation by activating the EGFR/Akt signaling pathway. PMID: 26261554
5. data suggests that DNA methylation contributes to down-regulation of DSC3 in prostate cancer, and loss of DSC3 predicts poor clinical outcome. PMID: 24664224
6. The data show an important role of physiologically occurring transcript d2-d4 in normal brain function. Interference with this role by DSC3 is a likely pathological mechanism in X-chromosomal dystonia parkinsonism syndrome PMID: 23184149
7. The p53 target gene desmocollin 3 acts as a novel tumor suppressor through inhibiting EGFR/ERK pathway in human lung cancer. PMID: 22941060
8. These findings demonstrate that IgG autoantibodies against an additional component of the desmosomes, Dsc3, induce loss of keratinocyte adhesion and thus may contribute to blister formation in pemphigus. PMID: 21281804
9. Methylation status of DSC3 DNA is a prognostic marker for colorectal cancer. P53 appears to have an important role in regulating DSC3 expression. PMID: 21364582
10. Autoimmunity to DSC3 in Pemphigus vulgaris. PMID: 20952584
11. Gene-profiling experiments of breast cancer cells infected with wt p53 revealed both MASPIN and desmocollin 3 (DSC3) to be p53-target genes, even though both genes are silenced in association with aberrant cytosine methylation of their promoters PMID: 12789271
12. Lss of DSC3 expression is a common event in primary breast tumor specimens, and DSC3 gene silencing in breast tumors is frequently linked to aberrant cytosine methylation and concomitant changes in chromatin structure. PMID: 16168112
13. Increased Dsc3 protein is associated with colorectal cancer PMID: 17088906
14. results suggest an abnormal expression of Dsc3, Dsg3, & beta-catenin induced in the progression of oral carcinomas & that Dsc3 expression level might be related to the regulation of beta-catenin in lymph node metastasis and cell proliferation in OSCCs. PMID: 17846785
15. Desmocollin-3 was expressed in almost half of the undifferentiated large-cell cancers PMID: 19287461
16. Both the binding of desmocollin 3 (Dsc3) to plakoglobin and Dsc3 phosphorylation are involved in Dsc3 binding to desmoglein 3 (Dsg3) during Ca2+ -induced desmosome assembly. PMID: 19348003
17. loss of heterophilic Dsc3/Dsg1 binding may contribute to pemphigus skin blistering PMID: 19717567
18. a human desmocollin-3 (DSC3) nonsense mutation underlies hereditary hypotrichosis and recurrent skin vesicles PMID: 19765682
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相關(guān)疾?。?/div>
Hypotrichosis and recurrent skin vesicles (HRSV)
亞細(xì)胞定位：

Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.
組織特異性：

Epidermis, buccal mucosa, esophagus and cervix.
數(shù)據(jù)庫鏈接：

HGNC: 3037

OMIM: 600271

KEGG: hsa:1825

STRING: 9606.ENSP00000353608

UniGene: Hs.41690

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