DNM2 Recombinant Monoclonal Antibody

說明書

中文名稱：

DNM2 Recombinant Monoclonal Antibody
貨號：

CSB-RA570688A0HU
規(guī)格：

￥1320
圖片：
Flow cytometric analysis of dynamin 2 expression in HT-1080 cells using dynamin 2 antibody. Green, isotype control; red, dynamin 2.

Immunocytochemical staining of HT-1080 cells with dynamin 2 antibody. Nuclei were stained blue with DAPI; Dynamin 2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using dynamin 2 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded mouse kidney using dynamin 2 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using dynamin 2 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dynamin 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

Uniprot No.：

P50570
基因名：

DNM2
別名：

DNM2; Dynamin 2; DYN2; DI-CMTB; CMTDIB; CMTDI1; DYNII; CMT2M; Dynamin II; EC 3.6.5.5; Dynamin-2; Cytoskeletal Protein; LCCS5
反應(yīng)種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human DNM2 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

9B3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, FC, ICC, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000
IHC	1:100-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane.
基因功能參考文獻(xiàn)：
1. DNM2 is a substrate for CDK1-dependent phosphorylation, which plays an important role in the regulation of human sperm acrosomal exocytosis. PMID: 29044420
2. Data found DNM2 highly expressed compared in both forms of ALL and associated with poor prognosis and with tumor cell proliferation. Also, Ikaros directly binds the DNM2 promoter and suppresses its expression. PMID: 27885263
3. a new AHI-1-BCR-ABL-DNM2 protein complex was uncovered, which regulates leukemic properties of these cells through a unique mechanism of cellular endocytosis and ROS-mediated autophagy. Thus, targeting this complex may facilitate eradication of LSCs for curative therapies PMID: 28366933
4. dynamin-2, as a tetramer, might help to establish hemi-fusion and stabilizes the pore during HIV-1 fusion. PMID: 28076788
5. Data suggest DNM2/RRAGB- (or DNM2/RRAGC-)dependent endocytosis of extracellular amino acids (AAs) plays critical role in mTORC1 transport/activation; recruitment of mTORC1 from cytoplasm to lysosome is suppressed by DNM2 inhibition; AA deprivation appears to be main cause of mTORC1 inactivation via DNM2 inhibition. (RHEB = Ras homolog enriched in brain; DNM2 = dynamin II; RRAG = Ras-related GTP binding protein) PMID: 28808055
6. We demonstrate that the dynamin2 effect on T cell adhesion does not involve integrin affinity regulation but instead relies on its ability to modulate integrin valency. PMID: 28273099
7. Thus, dynamin2 contributes to bladder cancer invasion by controlling invadopodia formation in bladder cancer cells and may prove a valuable therapeutic target. PMID: 27771248
8. Study showed that dynamin 2 and cortactin participate in the formation of F-actin bundles, which stabilize filopodia in migrating cancer cells. PMID: 27572123
9. Data demonstrate that dynamin II is required for the E2:ERalpha signaling of physiological functions and uncovers a role for autophagy in the control of ERalpha turnover. PMID: 27009360
10. This study reported the clinical characteristics, molecular diagnosis strategy, and DNM2 gene mutations of four Chinese Han patients with centronuclear myopathy. PMID: 26908122
11. Results demonstrate that overexpression of human DNM2 mRNAs, containing different disease-related mutations, cause a continuum of pathological features in zebrafish similarly to what observed in human centronuclear myopathies and neuropathies. PMID: 26842864
12. uncover a link between the dynamin 2 function and JNK signaling which leads to AP-1 induction PMID: 26475677
13. results provide evidence for a novel Arf6 activation mechanism by Dyn2 through EFA6B and EFA6D in CME in a manner dependent upon the GTPase activity of Dyn2 PMID: 26503427
14. We conclude that DNM2 is a novel negative regulator of NO production in mouse collecting ducts. PMID: 26791826
15. In marked contrast to invadopodia, this degradation does not require the action of Src kinase, Cdc42 or Dyn2. Rather, inhibition of Dyn2 causes a marked upregulation of stromal matrix degradation PMID: 25982272
16. exome sequencing family study reveals that autosomal dominant spastic paraplegia linked to a GTPase-effector domain mutation of dynamin 2 PMID: 26517984
17. This study demonistrated that DNM2 mutation releated to Centronuclear myopathy. PMID: 25957634
18. Dynamin 2 deletion in beta cells caused glucose intolerance and reduced the 2d phase of glucose-stimulated insulin secretion. Dynamin 2 regulates insulin secretory capacity and dynamics in vivo via a mechanism depending on CME and F-actin remodeling. PMID: 26413867
19. this study described for the first time the clinical, pathological and genetic features of DNM2-related CNM in Chinese patients. PMID: 25501959
20. Data indicate that dynamin 2 (Dyn2) interacts with Connexin 26 (Cx26) in a yeast two-hybrid screen, and co-localizes in mammalian cells. PMID: 25263585
21. Dynamin 2 is recruited as dimers during endocytic membrane scission at the neck of a clathrin-coated pit. PMID: 25232009
22. Thus dynamin that was recently found to control late stages of myoblast fusion also controls late stages of macrophage fusion, revealing an intriguing conserved mechanistic motif shared by diverse cell-cell fusion processes. PMID: 25336256
23. FGF21 has a role in promoting endothelial cell angiogenesis through a dynamin-2 and Rab5 dependent pathway PMID: 24848261
24. Inhibition of Dyn2 prevents cell invasiveness in androgen-responsive and -refractory PCA models, supporting the potential benefit of Dyn2 to serve as a therapeutic target for advanced prostate cancer . PMID: 24402972
25. These results reveal that girdin regulates selective clathrin-mediated endocytosis via a mechanism involving dynamin 2, but not by operating as a cargo-specific adaptor. PMID: 25061227
26. Upon overexpression of dynamin-2 mutants or depletion of clathrin heavy chain, flotillins are permanently trapped in endosomes. PMID: 24809731
27. dynamin2 recruitment is regulated by actin polymerization PMID: 24891602
28. Results indicate that hSNF5 affects both the stability and the activity of DNM2, uncovering an unexpected role of hSNF5 in modulating endocytosis, and open new perspectives in understanding the role of hSNF5 in tumour genesis. PMID: 23851497
29. results demonstrate a regulatory relationship between DNM2, miR-199a, and HIF, with implications in cancer metastasis PMID: 24706848
30. A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes. PMID: 24016602
31. These findings provide new evidence for the participation of the autolysosome in hepatocyte lipid droplet metabolism and demonstrate a novel role for dynamin2 in the function and maturation of an autophagic compartment. PMID: 24145164
32. Dual role of BAR domain-containing proteins in regulating vesicle release catalyzed by the GTPase, dynamin-2. PMID: 23861397
33. We identify dynamin and the EAP-binding alpha-adaptin appendage domain of the AP2 adaptor as switches in a regulated, multistep maturation process and provide direct evidence for a molecular checkpoint in clathrin mediated endocytosis. PMID: 23891661
34. The first homozygous mutation in the DNM2 protein p.Phe379Val, in three consanguineous patients with a lethal congenital syndrome, is reported. PMID: 23092955
35. Three novel DNM2 mutations in an Italy cohort of centronuclear myopathy patients. PMID: 23394783
36. This mutation (D614N) within the DNM2 gene in a large centronuclear myopathy family with a late age of overt clinical manifestation caused profound changes in DNM2 localization and impaired proper organization of myofibers and skeletal muscle function. PMID: 23374900
37. New onset strabismus and a history of a myopathy should consider this entity in the differential diagnosis that could be confirmed by a muscle biopsy and mutational analysis. PMID: 22924779
38. study provides evidence the large GTPase Dyn2 regulates the small GTPase Rac1 to potentiate invasive migration of pancreatic tumor cells; Dyn2 plays an essential role in regulating Rac1-mediated pancreatic tumor cell migration through modulation of the Rac1 activator Vav1 via a direct interaction PMID: 23537630
39. investigation of dynamin 2 expression in cervix of patients with suspected cervical intraepithelial neoplasia; evaluation of dynamin 2 expression as biological marker in diagnosis/staging/grading of cervical intraepithelial neoplasia PMID: 22959143
40. Dynamin II function is required for EGF-mediated Stat3 activation but not Erk1/2 phosphorylation PMID: 22574813
41. clinicopathological features of DNM2 centronuclear myopathy are rather homogeneous and can be distinguished from the features of non-DNM2 centronuclear myopathy PMID: 22613877
42. Caveolin-1 and dynamin-2 are essential for removal of the complement C5b-9 complex via endocytosis. PMID: 22528500
43. Defects in membrane trafficking due to DNM2 mutations potentially represent a common pathological mechanism in CNM and CMT. PMID: 22396310
44. DNM2 mutations cause autosomal dominant centronuclear myopathy or autosomal dominant (AD) Charcot-Marie-Tooth (CMT) disease; one large Czech family with 15 members affected with an AD CMT phenotype of extraordinary variability is reported. PMID: 22091729
45. this study identifies Dyn2 as an effector that mediates PDGFRalpha-SHP-2-induced glioma tumor growth and invasion PMID: 21996738
46. tumor cells overexpressing Dyn2 protruded lamellipodia at twice the rate, migrated faster (180%) and farther (2.5-fold greater distance) on glass and through transwell chambers PMID: 21841817
47. The alteration of microtubules by dynamin2 depletion reduced the length and the speed of the actin comet. PMID: 22174845
48. The mild functional defects are suggestive of differences between CMT and CNM disease-causing dynamin 2 mutants and suggest that a slight impairment in clathrin-mediated pathways may accumulate over time to foster the respective human diseases. PMID: 22096584
49. In summary, these results suggested that dynamin 2 might play a role in HIV-1 Env-mediated cell-cell fusion. PMID: 21338326
50. The deletion of PRD domain of dynamin 2 resulted in the impairment of both the localization and the abscission of daughter cells. PMID: 21150131
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相關(guān)疾病：

Myopathy, centronuclear, 1 (CNM1); Lethal congenital contracture syndrome 5 (LCCS5); Charcot-Marie-Tooth disease, dominant, intermediate type, B (CMTDIB); Charcot-Marie-Tooth disease 2M (CMT2M)
亞細(xì)胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Cell junction. Membrane, clathrin-coated pit. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Midbody. Cell projection, phagocytic cup. Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein.
蛋白家族：

TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
組織特異性：

Ubiquitously expressed.
數(shù)據(jù)庫鏈接：

HGNC: 2974

OMIM: 160150

KEGG: hsa:1785

STRING: 9606.ENSP00000347890

UniGene: Hs.211463

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