DBF4 Recombinant Monoclonal Antibody

說明書

中文名稱：

DBF4重組抗體
貨號：

CSB-RA155202A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate
All lanes: DBF4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 77, 27 kDa
Observed band size: 72-95 kDa

Immunofluorescence staining of A549 cell with CSB-RA155202A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 551-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA155202A0HU重組單克隆抗體是針對DBF4蛋白的高特異性科研工具，適用于ELISA、Western Blot（WB）和免疫熒光（IF）檢測。DBF4作為DNA復制起始的關鍵調控因子，通過與CDC7激酶形成復合物激活其激酶活性，在細胞周期S期啟動、DNA復制檢驗點調控及基因組穩定性維持中發揮重要作用。經多平臺驗證，該抗體在WB實驗中可檢測到目標蛋白的清晰特異性條帶，推薦使用稀釋度為1:500-1:2000；在IF實驗中能精準定位細胞內靶蛋白，推薦稀釋度1:50-1:200，其嚴格的質控標準確保了實驗結果的可靠性和重復性。該產品適用于研究DNA復制調控機制、細胞周期異常相關疾病（如腫瘤發生發展）以及基因組穩定性相關信號通路，為分子生物學、細胞生物學等基礎研究領域提供高效工具。所有實驗數據均經陽性和陰性對照驗證，滿足科研實驗對特異性、靈敏度及穩定性的高標準需求。
Uniprot No.：

Q9UBU7
基因名：

DBF4
別名：

Protein DBF4 homolog A (Activator of S phase kinase) (Chiffon homolog A) (DBF4-type zinc finger-containing protein 1), DBF4, ASK DBF4A ZDBF1
反應種屬：

Human
免疫原：

A synthesized peptide derived from human DBF4
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

24D8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
基因功能參考文獻：
1. Both CDC7 and DBF4 promoters bind E2F, suggesting that increased E2F activity in RB1 mutant cancers promotes increased DDK expression. Surprisingly, increased DDK expression levels are also correlated with both increased chemoresistance and genome-wide mutation frequencies. PMID: 28448802
2. Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication. PMID: 27341472
3. we propose that phosphorylation of TOP2A by CDC7/DBF4 in early S-phase prevents its localization and/or activity at centromeres, and inhibition of TOP2A function could be relevant to prevent premature separation of centromeric DNA. PMID: 27407105
4. The anti-invasive and cell cycle arrest-inducing effects of nitrogen-containing bisphosphonates are not DBF4 mediated in human breast cancer cells. PMID: 24287290
5. Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint. PMID: 22123827
6. bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. PMID: 21536671
7. Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction PMID: 21036905
8. Data document the role of DBF4 as a key player in nitrogen-containing bisphosphonate-induced cytotoxicity, thus explaining the effects on the cell-cycle. PMID: 19744312
9. The interaction with LEDGF relieves autoinhibition of Cdc7-ASK kinase, imposed by the C terminus of ASK. PMID: 19864417
10. results suggest that E2F regulates the ASK promoter through an atypical mode of recognition of the target site PMID: 12015319
11. The HuDbf4 gene has of 12 exons over a 33-kb region. The MluI cell-cycle box is needed for core promoter activation. The Sp1 upregulator & HES-1 repressor coordinately determine promoter efficiency. Transcription initiations occur at -220, -235 and -245. PMID: 12420215
12. Results demonstrate a DNA damage checkpoint that targets Cdc7/Dbf4 protein kinase. PMID: 12535533
13. Cdc7-Dbf4 kinase efficiently phosphorylates p150. PMID: 16826239
14. Taken together, these results indicate that Cdc7/Dbf4 phosphorylation of MCM2 is essential for the initiation of DNA replication in mammalian cells. PMID: 16899510
15. Overexpression of Dbf4 abrogates the S checkpoint response to ultraviolet radiation but not ionizing radiation. PMID: 17276990
16. There is the differential regulation of a novel gene, namely ASK/Dbf4, in melanoma and suggest that up-regulation of ASK/Dbf4 is a novel molecular determinant with prognostic relevance that confers a proliferative advantage in cutaneous melanoma. PMID: 17768177
17. Cdc7/Dbf4 kinase activity inhibition affects specific phosphorylation sites on MCM2 in cancer cells PMID: 18286467
18. ASK/Dbf4, a novel cell survival gene in melanoma is transcriptionally regulated by E2F1. PMID: 18503552
19. A strong origin of replication at the DBF4 promoter locus, which contains two initiation zones, two origin recognition complex (ORC) binding sites and two DNase I-hypersensitive regions, is identified. PMID: 18536724
20. increased Cdc7-Dbf4 abundance may be a common occurrence in human malignancies PMID: 18714392
21. These results indicate that Ddk functions as an upstream regulator to monitor S-phase checkpoint signaling. PMID: 19111665
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亞細胞定位：

Nucleus.
組織特異性：

Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
數據庫鏈接：

HGNC: 17364

OMIM: 604281

KEGG: hsa:10926

STRING: 9606.ENSP00000265728

UniGene: Hs.485380

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