CALB2 Recombinant Monoclonal Antibody

說明書

中文名稱：

CALB2 Recombinant Monoclonal Antibody
貨號：

CSB-RA193105A0HU
規格：

￥1320
圖片：
Immunofluorescence staining of SH-SY5Y cell with CSB-RA193105A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA193105A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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產品詳情
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靶點詳情

產品詳情

Uniprot No.：

P22676
基因名：

CALB2
別名：

Calretinin (CR) (29 kDa calbindin), CALB2, CAB29
反應種屬：

Human
免疫原：

A synthesized peptide from human CALB2 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

11F9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Calretinin is a calcium-binding protein which is abundant in auditory neurons.
基因功能參考文獻：
1. we have identified septin 7, an essential cellular component implicated in the final steps of cell division, as a strong Bt-dependent gene regulatory protein binding to the promoter of CALB2. PMID: 29699512
2. Calretinin was positive in 20 cases with MM. PMID: 27761727
3. Calretinin could be verified as a blood-based marker for Malignant mesothelioma (MM). The assay is robust and shows a performance that is comparable to that of mesothelin. Retrospective analyses would not be limited by storage time. The high specificity supports a combination of calretinin with other markers. PMID: 28558669
4. calretinin increases Leydig cell viability and proliferation, possibly via ERK1/2 and AKT pathways, and suppresses apoptosis possibly via the mitochondria-related apoptotic pathway PMID: 28437248
5. expression of calretinin in human fetal testis specimens, was examined. PMID: 27990638
6. In Malignant mesothelioma , increased calretinin expression is associated with epithelioid histology and better survival. PMID: 27780599
7. Calretinin, D2-40 and mesothelin are aberrantly expressed in a proportion of CRC cases. PMID: 27062033
8. We studied 38 bone marrow biopsy specimens of non-CML myeloproliferative neoplasms from 31 patients. All eight bone marrow biopsy specimens from patients with mutant CALR showed strong cytoplasmic staining of the megakaryocytes (83.5%; range, 50%-98%; median, 87%) with the CAL2 antibody. PMID: 27686170
9. NCX1 and the calcium binding protein calretinin cooperate within the striatum to confer tolerance against cerebral ischemia. PMID: 25633096
10. Overexpression of human calretinin in mouse primary mesothelial cells inversely affects proliferation and cell migration. PMID: 26695618
11. Patients with Down syndrome may have enteric nervous system disorders functionally similar to HD, related to abnormal or imbalanced autonomic innervation, of which distal calretinin hypoinnervation is one manifestation, despite the presence of ganglia. PMID: 26230373
12. the unique disposition of CALR at the MII spindle uncovers a physical interaction with endoplasmic reticulum that may mediate cytoskeletal remodelling during oocyte maturation. PMID: 25620022
13. In focal cortical dysplasia (FCD), the density of CR positive neurons was increased in cortical layer V compared to non-FCD temporal lobe. PMID: 26081613
14. might be used as a marker for the ameloblastomas and could play an important role in the differentiation of aggressiveness of different odontogenic tumors PMID: 25046230
15. We concluded that WT1, but not calretinin, is a useful prognostic factor in malignant pleural mesothelioma PMID: 24323466
16. This article reviews the information that is currently available on calretinin expression in tumors and on its application as an immunohistochemical marker in diagnostic pathology. PMID: 24185118
17. Calretinin positivity was observed in all rectal biopsies of Hirschsprung disease showing the presence of ganglion cells, and the staining was consistently absent in all cases of aganglionosis. PMID: 25119007
18. Calretinin IHC offered additional diagnostic value in the Hirschprung disease using specimens with inadequate amount of submucosa and rarely seen ganglion cells. PMID: 24294384
19. calretinin immunoexpression and the hormonal status for estrogen receptors (ER), progesterone receptors (PR), and ERRB2, in 33 cases of cutaneous metastases of ductal breast carcinoma, as well as in seven primary cutaneous apocrine tumors, were studied PMID: 24322014
20. Restricted diffusion of calretinin in cerebellar granule cell dendrites implies Ca(2)-dependent interactions via its EF-hand 5 domain. PMID: 23732647
21. Calretinin was found in nerve fibrils in all of the fetal colonic samples. Calretinin was expressed in the gut as early as 22 gestational weeks. PMID: 23701779
22. CD34/calretinin immunohistochemistry could be useful for differentiating sinonasal schwannoma from its histological mimics. PMID: 23995573
23. high-level calretinin expression in invasive ductal carcinoma of the breast is most common in grade 3 tumors with a basal-like phenotype and is associated with poor overall survival. PMID: 24262018
24. Data indicate that fallopian tube and the epithelial component of Brenner tumours (BTs) expressed AKR1C3 and androgen receptor, but the tumour stromal cells showed strong expression of calretinin, inhibin and steroidogenic factor 1 in the majority of BTs. PMID: 24012099
25. All 12 patients without Hirschsprung disease had calretinin-positive nerve fibers in the lamina propria or muscularis mucosae, and all 5 patients with Hirschsprung disease had no calretinin staining of nerves. PMID: 23899067
26. High Calretinin expression is associated with mesothelioma. PMID: 23595591
27. Our results show that different levels of serous ovarian tumor progression are accompanied by changes in the immunohistochemical expression pattern of EpCAM, CD24, and calretinin PMID: 23011824
28. an unexpected role for CR in directly modulating effectors such as Ca(v)2.1, which may have major consequences for Ca(2+) signaling and neuronal excitability. PMID: 23033479
29. This study demonistrated that significant linear regression between calbindin expression and short-term and long-term percent retention of auditory verbal learning test in patients whose epilepsy PMID: 22796338
30. Data show that calretinin and CK5/6 were positive in 100 and 64% of mesotheliomas, and 92 and 31% of reactive effusions, respectively, and desmin was negative in all malignant cases and positive in 85% of reactive effusions. PMID: 23075894
31. Over-expression of CR reduces huntingtin-caused cytotoxicity in both non-neuronal and neuronal cell models of Huntington's disease. PMID: 22891683
32. A set of cryosections revealed extensive colocalization of calretinin with vasoactive intestinal peptide in both surviving submucosal perikarya and mucosal nerve fibres in human chagasic submucosa and mucosa. PMID: 22555304
33. Immunohistochemical study of calretinin expression in normal skin and cutaneous adnexal proliferations. PMID: 22343111
34. Low calretinin is associated with high neutrophil-to-lymphocyte ratio and malignant mesothelioma. PMID: 22011651
35. Calretinin appears to be a useful marker to distinguish Olfactory neuroblastoma from other small round blue cell tumors PMID: 22020045
36. Results suggest that following 5-FU treatment in CRC cell lines, CALB2 is involved in apoptosis induction through the intrinsic mitochondrial pathway. This indicates that CALB2 may be an important mediator of 5-FU-induced cell death PMID: 21629658
37. calretinin-containing cells in the human hippocampus are highly vulnerable, thus inhibition mediated by dendritic inhibitory cells and their synchronization by interneuron-specific interneurons may be impaired in epilepsy. PMID: 20576695
38. Developed a novel and sensitive ELISA for the detection of calretinin in serum, heparin plasma, or EDTA plasma augmented with calcium from mesothelioma patients. PMID: 20509881
39. identification of two elements that act as butyrate-sensitive repressors in all colon cancer cell lines tested PMID: 19998412
40. The overall density of Calretinin neurons in cerebral cortex was significantly decreased in focal cortical dysplasias. PMID: 19854615
41. Calretinin plays a crucial role in the modulation of intrinsic neuronal excitability and the induction of long-term potentiation. PMID: 19450707
42. a tumor marker for cardiac myxoma PMID: 11642722
43. Two types of calretinin-immunostained terminals were found in the CA1 region. PMID: 12064772
44. AP2-like cis element acts as an enhancer of gene expression in neuronal cells PMID: 12359331
45. E-cadherin and calretinin are sensitive and specific in differential diagnosis of benign and malignant serous effusion specimens. PMID: 15285029
46. Disorders of Sertoli cell differentiation as indicated by calretinin and/or cytokeratin-18 expression contribute to the multifactorial mechanisms underlying spermatogenic failure PMID: 15950053
47. Calretinin-immunoreactive unipolar brush cells are presented in the vermis after the 28th gestational week. At birth, they are presentm in the vestibular lobules. At the birth the number of calretinin-immunoreactive unipolar brush cells increase. PMID: 16289944
48. Calretinin immunostaining provides some insights into the histogenesis of invasive and noninvasive implants in serous borderline tumors of the ovary. PMID: 16415795
49. Cells lining the mesothelial inclusion cysts had CAL2 immunohistochemical reactivity. PMID: 17535091
50. The combinational characterization of calretinin (either by polyclonal or monoclonal antibody), inhibin alpha, and Melan-A expression is of great significance in the differential diagnosis of adrenocortical tumors. PMID: 18091323
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蛋白家族：

Calbindin family
組織特異性：

Brain.
數據庫鏈接：

HGNC: 1435

OMIM: 114051

KEGG: hsa:794

STRING: 9606.ENSP00000307508

UniGene: Hs.106857

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