VPS26B Antibody

中文名稱：

VPS26B兔多克隆抗體
貨號：

CSB-PA676824LA01HU
規格：

￥3900
圖片：
Western Blot
Positive WB detected in: K562 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MCF7 whole cell lysate, A431 whole cell lysate
All lanes: VPS26B antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa

IHC image of CSB-PA676824LA01HU diluted at 1:50 and staining in paraffin-embedded mouse brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.

Immunofluorescence staining of Hela cell with CSB-PA676824LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) VPS26B Polyclonal antibody
Uniprot No.：

Q4G0F5
基因名：

VPS26B
別名：

Pep8b antibody; Vacuolar protein sorting 26 homolog B (S. pombe) antibody; Vacuolar protein sorting 26 homolog B (yeast) antibody; Vacuolar protein sorting-associated protein 26B antibody; Vesicle protein sorting 26B antibody; VP26B_HUMAN antibody; VPS26B antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Vacuolar protein sorting-associated protein 26B protein (1-336AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，VPS26B Antibody (CSB-PA676824LA01HU)，的標記方式是Non-conjugated。對于VPS26B Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA676824LB01HU	VPS26B Antibody, HRP conjugated	ELISA
FITC	CSB-PA676824LC01HU	VPS26B Antibody, FITC conjugated
Biotin	CSB-PA676824LD01HU	VPS26B Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen?affinity?purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative:?0.02%?sodium?azide
Constituents:?PBS?containing?50%?glycerol pH 7.3
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	?1:20-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. May be involved in retrograde transport of SORT1 but not of IGF2R. Acts redundantly with VSP26A in SNX-27 mediated endocytic recycling of SLC2A1/GLUT1.
基因功能參考文獻：
1. Authors propose that PAR-2 plasma membrane repopulation is regulated by Vps26B-retromer, describing a potential novel role for this complex. PMID: 26113136
2. Colocalization of Vps26 paralogues with different endosomally located Rab proteins shows prolonged association of Vps26B-retromer with maturing endosomes relative to Vps26A-retromer. PMID: 21920005
亞細胞定位：

Cytoplasm. Membrane; Peripheral membrane protein. Early endosome. Late endosome.
蛋白家族：

VPS26 family
數據庫鏈接：

HGNC: 28119

OMIM: 610027

KEGG: hsa:112936

STRING: 9606.ENSP00000281187

UniGene: Hs.334684

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