TOP1MT Antibody

中文名稱：

TOP1MT兔多克隆抗體
貨號：

CSB-PA853372LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA853372LA01HU diluted at 1:600 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) TOP1MT Polyclonal antibody
Uniprot No.：

Q969P6
基因名：

TOP1MT
別名：

TOP1MT antibody; DNA topoisomerase I antibody; mitochondrial antibody; TOP1mt antibody; EC 5.6.2.1 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，TOP1MT Antibody (CSB-PA853372LA01HU)，的標記方式是Non-conjugated。對于TOP1MT Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA853372LB01HU	TOP1MT Antibody, HRP conjugated	ELISA
FITC	CSB-PA853372LC01HU	TOP1MT Antibody, FITC conjugated
Biotin	CSB-PA853372LD01HU	TOP1MT Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
基因功能參考文獻：
1. This report was the first to focus on the role of TOP1MT in tumors. We identified a new mechanism underlying TOP1MT-deficient gastric cancer (GC) metastasis and showed that TOP1MT played an important role in cancer cells. PMID: 28874393
2. Data indicate that the different coordination geometry achieved by the two transition metal ions has an important role in modulating their efficiency as topoisomerase I inhibitors. PMID: 24172750
3. DNA topoisomerase I stimulates BLM helicase activity on a nucleolar-relevant RNA:DNA hybrid. PMID: 23261817
4. Data reported in this paper indicate that hTop IB does not confer to the cells radio-resistance, but the kinetic analysis of both DNA repair rate as well as colonies growth, demonstrates that the cells containing hTop IB show a more efficient rescue from IR injury. PMID: 23422507
亞細胞定位：

Mitochondrion.
蛋白家族：

Type IB topoisomerase family
組織特異性：

Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.
數據庫鏈接：

HGNC: 29787

OMIM: 606387

KEGG: hsa:116447

STRING: 9606.ENSP00000328835

UniGene: Hs.528574

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