The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA125082(TOP1MT Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA125082(TOP1MT Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
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貨期:
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用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
基因功能參考文獻:
This report was the first to focus on the role of TOP1MT in tumors. We identified a new mechanism underlying TOP1MT-deficient gastric cancer (GC) metastasis and showed that TOP1MT played an important role in cancer cells. PMID: 28874393
Data indicate that the different coordination geometry achieved by the two transition metal ions has an important role in modulating their efficiency as topoisomerase I inhibitors. PMID: 24172750
DNA topoisomerase I stimulates BLM helicase activity on a nucleolar-relevant RNA:DNA hybrid. PMID: 23261817
Data reported in this paper indicate that hTop IB does not confer to the cells radio-resistance, but the kinetic analysis of both DNA repair rate as well as colonies growth, demonstrates that the cells containing hTop IB show a more efficient rescue from IR injury. PMID: 23422507
亞細胞定位:
Mitochondrion.
蛋白家族:
Type IB topoisomerase family
組織特異性:
Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver.