RFXANK Antibody

中文名稱：

RFXANK兔多克隆抗體
貨號：

CSB-PA019617LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: 293T whole cell lysate, SH-SY5Y whole cell lysate
All lanes: RFXANK antibody at 6.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29, 25, 26 kDa
Observed band size: 29 kDa

IHC image of CSB-PA019617LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of PC-3 cells with CSB-PA019617LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) RFXANK Polyclonal antibody
Uniprot No.：

O14593
基因名：

RFXANK
別名：

ANKRA1 antibody; Ankyrin repeat containing regulatory factor X associated protein antibody; Ankyrin repeat family A protein 1 antibody; BLS antibody; DNA-binding protein RFXANK antibody; F14150_1 antibody; MGC138628 antibody; Regulatory factor X associated ankyrin containing protein antibody; Regulatory factor X subunit B antibody; Regulatory factor X, ankyrin repeat containing antibody; Regulatory factor X-associated ankyrin-containing protein antibody; RFX Bdelta4 antibody; RFX-B antibody; RFXANK antibody; RFXB antibody; RFXB delta 4 antibody; RFXK_HUMAN antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human DNA-binding protein RFXANK protein (3-84AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，RFXANK Antibody (CSB-PA019617LA01HU)，的標記方式是Non-conjugated。對于RFXANK Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA019617LB01HU	RFXANK Antibody, HRP conjugated	ELISA
FITC	CSB-PA019617LC01HU	RFXANK Antibody, FITC conjugated
Biotin	CSB-PA019617LD01HU	RFXANK Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator/CIITA. May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. May also potentiate the activation of RAF1.; Isoform 2 is not involved in the positive regulation of MHC class II genes.
基因功能參考文獻：
1. mutation results in Bare Lymphocyte Syndrome, Type 2 in a child of Mexican descent PMID: 26634365
2. genetic, clinical, and immunologic features of 35 patients from 30 unrelated kindreds from North Africa sharing the same RFXANK founder mutation, a 26-bp deletion called I5E6-25_I5E6 + 1) PMID: 21908431
3. Findings confirm the association between the high frequency of the combined immunodeficiency and the defect in MHC class II expression and provides strong evidence for a founder effect of the 752delG26 mutation in the North African population. PMID: 20414676
4. Low levels of RFX-B are found in macrophages of colorectal cancer patients, partly explaining immunodeficiency in cancer. PMID: 11836625
5. in vivo effects of mutations on the expression of the RFXANK RNA and protein PMID: 12618906
6. ANKRA, RFXANK, and CIITA are novel targets of class IIa HDACs which may deacetylases play a role in regulating MHCII expression PMID: 15964851
7. analysis of RFXANK domains and function PMID: 16166641
8. RFXAP and RFXB have roles in relieving autoinhibition of RFX5 PMID: 18723135
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相關疾病：

Bare lymphocyte syndrome 2 (BLS2)
亞細胞定位：

Cytoplasm. Nucleus.
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 9987

OMIM: 209920

KEGG: hsa:8625

STRING: 9606.ENSP00000305071

UniGene: Hs.153629

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