PRUNE1 Antibody
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中文名稱：PRUNE1兔多克隆抗體
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貨號：CSB-PA771430LA01HU
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規格：￥440
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圖片：
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate
All lanes: PRUNE1 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51, 43, 31, 27, 25, 19 kDa
Observed band size: 60 kDa -
IHC image of CSB-PA771430LA01HU diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA771430LA01HU at 1:135, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunoprecipitating PRUNE1 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA771430LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA771430LA01HU (8µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (10µg)
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) PRUNE1 Polyclonal antibody
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Uniprot No.：
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基因名：PRUNE1
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別名：DRES-17 antibody; DRES17 antibody; Drosophila-related expressed sequence 17 antibody; hPrune antibody; HTcD37 antibody; Protein prune homolog antibody; PRUNE antibody; Prune homolog antibody; PRUNE like protein antibody; PRUNE_HUMAN antibody; TcD37 homolog antibody
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宿主：Rabbit
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反應種屬：Human
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免疫原：Recombinant Human Exopolyphosphatase PRUNE1 protein (1-168AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產品，PRUNE1 Antibody (CSB-PA771430LA01HU)，的標記方式是Non-conjugated。對于PRUNE1 Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 IHC 1:200-1:500 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.
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基因功能參考文獻：
- PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. PMID: 28334956
- These results further delineate a new PRUNE1-related syndrome, and highlight the importance of periodic data re-annotation in individuals who remain without a diagnosis after undergoing genome-wide testing. PMID: 28211990
- h-Prune expression is necessary for cancer cell motility and EMT and is associated with liver and lung metastasis in colorectal cancer cells. h-Prune could be a new prognostic marker and molecular target for CRLM. PMID: 27037526
- Together these studies define PRUNE as a molecule fundamental for normal human cortical development and define cellular and clinical consequences associated with PRUNE mutation PMID: 28334956
- h-prune is frequently expressed in anaplastic thyroid cancer cells and lymph nodes metastasis, and promotes migration and invasion of anaplastic thyroid cancer cells and metastasis in an anaplastic thyroid cancer model. PMID: 27109060
- Authors assessed h-Prune levels in peripheral blood of lung cancer patients using ELISA assay, showing that h-Prune is an early diagnostic marker for lung cancer. PMID: 25026278
- Data indicate that the D388A and D422A mutant h-Prune proteins interact weakly with Nm23-H1. PMID: 23448979
- Increased amplification of PRUNE is associated with T4 breast carcinoma. PMID: 20735841
- prune has a role in breast neoplasm aggressiveness PMID: 15671547
- GSK-3 and h-prune cooperatively regulate the disassembly of focal adhesions to promote cell migration and that h-prune is useful as a marker for tumor aggressiveness. PMID: 16428445
- Prune is composed of two independent active sites and two interaction sites for the assembly of oligomeric signalling complexes PMID: 17655525
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相關疾病：Neurodevelopmental disorder with microcephaly, hypotonia, and variable brain anomalies (NMIHBA)
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亞細胞定位：Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed.
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蛋白家族：PPase class C family, Prune subfamily
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組織特異性：Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.
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數據庫鏈接：
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