Human Protein prune homolog(PRUNE) ELISA kit

Human Protein prune homolog(PRUNE) ELISA kit

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中文名稱：

人蛋白prune同源物(PRUNE)酶聯免疫試劑盒
貨號：

CSB-EL018831HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人蛋白prune同源物(PRUNE)酶聯免疫試劑盒（CSB-EL018831HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PRUNE含量。試劑盒檢測范圍為47 pg/mL-3000 pg/mL，適用于科研領域中對PRUNE蛋白功能的機制研究，例如探究其在癌癥轉移微環境中的作用、神經退行性疾病相關信號通路調控，或結合細胞模型分析藥物干預對PRUNE表達的影響本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

DRES-17 ELISA Kit; DRES17 ELISA Kit; Drosophila-related expressed sequence 17 ELISA Kit; hPrune ELISA Kit; HTcD37 ELISA Kit; Protein prune homolog ELISA Kit; PRUNE ELISA Kit; Prune homolog ELISA Kit; PRUNE like protein ELISA Kit; PRUNE_HUMAN ELISA Kit; TcD37 homolog ELISA Kit
縮寫：

PRUNE
Uniprot No.：

Q86TP1
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

47 pg/mL-3000 pg/mL
靈敏度：

11.75 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PRUNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-94
1:2	Average %	96
	Range %	91-104
1:4	Average %	89
	Range %	93-96
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of human PRUNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.283	2.183	2.233	2.111
1500	1.647	1.567	1.607	1.485
750	0.982	0.952	0.967	0.845
375	0.532	0.512	0.522	0.400
187.5	0.384	0.374	0.379	0.257
94	0.247	0.237	0.242	0.120
47	0.199	0.189	0.194	0.072
0	0.122	0.121	0.122

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, migration and differentiation, and acts as a negative regulator of NME1. Plays a role in the regulation of neurogenesis. Involved in the regulation of microtubule polymerization.
基因功能參考文獻：
1. PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. PMID: 28334956
2. These results further delineate a new PRUNE1-related syndrome, and highlight the importance of periodic data re-annotation in individuals who remain without a diagnosis after undergoing genome-wide testing. PMID: 28211990
3. h-Prune expression is necessary for cancer cell motility and EMT and is associated with liver and lung metastasis in colorectal cancer cells. h-Prune could be a new prognostic marker and molecular target for CRLM. PMID: 27037526
4. Together these studies define PRUNE as a molecule fundamental for normal human cortical development and define cellular and clinical consequences associated with PRUNE mutation PMID: 28334956
5. h-prune is frequently expressed in anaplastic thyroid cancer cells and lymph nodes metastasis, and promotes migration and invasion of anaplastic thyroid cancer cells and metastasis in an anaplastic thyroid cancer model. PMID: 27109060
6. Authors assessed h-Prune levels in peripheral blood of lung cancer patients using ELISA assay, showing that h-Prune is an early diagnostic marker for lung cancer. PMID: 25026278
7. Data indicate that the D388A and D422A mutant h-Prune proteins interact weakly with Nm23-H1. PMID: 23448979
8. Increased amplification of PRUNE is associated with T4 breast carcinoma. PMID: 20735841
9. prune has a role in breast neoplasm aggressiveness PMID: 15671547
10. GSK-3 and h-prune cooperatively regulate the disassembly of focal adhesions to promote cell migration and that h-prune is useful as a marker for tumor aggressiveness. PMID: 16428445
11. Prune is composed of two independent active sites and two interaction sites for the assembly of oligomeric signalling complexes PMID: 17655525
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相關疾病：

Neurodevelopmental disorder with microcephaly, hypotonia, and variable brain anomalies (NMIHBA)
亞細胞定位：

Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed.
蛋白家族：

PPase class C family, Prune subfamily
組織特異性：

Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.
數據庫鏈接：

HGNC: 13420

OMIM: 617413

KEGG: hsa:58497

STRING: 9606.ENSP00000271620

UniGene: Hs.78524

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.