NEDD1 Antibody
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中文名稱：NEDD1兔多克隆抗體
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貨號：CSB-PA822820DSR1HU
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規格：￥440
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圖片：
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Western blot
All lanes: NEDD1 antibody at 3.25μg/ml
Lane 1: Mouse small intestine tissue
Lane 2: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 72, 63, 73 kDa
Observed band size: 72 kDa -
Immunohistochemistry of paraffin-embedded human glioma using CSB-PA822820DSR1HU at dilution of 1:100 -
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA822820DSR1HU at dilution of 1:100 -
Immunoprecipitating NEDD1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA822820DSR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA822820DSR1HU (4μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) NEDD1 Polyclonal antibody
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Uniprot No.：
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基因名：NEDD1
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別名：FLJ35902 antibody; GCP WD antibody; NEDD-1 antibody; NEDD1 antibody; NEDD1_HUMAN antibody; Neural precursor cell expressed developmentally down-regulated protein 1 antibody; Neural precursor cell expressed, developmentally down regulated gene 1 antibody; Protein NEDD1 antibody; TUBGCP7 antibody
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宿主：Rabbit
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反應種屬：Human, Mouse
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免疫原：Recombinant Human Protein NEDD1 protein (411-660AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen Affinity Purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 IHC 1:20-1:200 IP 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.
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基因功能參考文獻：
- One important function of Aurora A in mitotic cells is to promote microtubule nucleation around the chromatin by phosphorylating NEDD1, and thereby to promote functional spindle assembly. PMID: 23273898
- The mice treated with small interfering (si) RNA targeting NEDD1 by the atelocollagen-mediated delivery system showed a significantly prolonged survival in a xenograft model of human gastric cancer. PMID: 23106787
- Data suggest that the NEDD1/gamma-tubulin interaction is finely tuned by multiple phosphorylation events in the S557-S574 region and is critical for spindle assembly. PMID: 22595525
- a direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome PMID: 20224777
- GCP-WD (NEDD1) broadly mediates targeting of the gamma-tubulin ring complex to sites of microtubule nucleation and to the mitotic spindle, which is essential for spindle formation. PMID: 16378099
- The NEED1 protein was characterized and its function in centriole duplication and spindle assembly determined. PMID: 16461362
- Data propose NEDD1 may be a promising target for controlling cell proliferation, in particular if targeted in combination with Plk1 inhibitors. PMID: 19243593
- Data suggest that sequential phosphorylation of Nedd1 by Cdk1 and Plk1 plays a role in targeting gamma tubulin ring complex to the centrosome by promoting the interaction of Nedd1 with the gammaTuRC component gamma-tubulin, during mitosis. PMID: 19509060
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亞細胞定位：Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
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數據庫鏈接：
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