METAP2 Antibody
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中文名稱：METAP2兔多克隆抗體
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貨號：CSB-PA013716LA01HU
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規格：￥440
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圖片：
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Western Blot
Positive WB detected in: Rat liver tissue
All lanes: METAP2 antibody at 3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53, 52, 51 kDa
Observed band size: 53 kDa -
IHC image of CSB-PA013716LA01HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA013716LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) METAP2 Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：A930035J23Rik antibody; AI047573 antibody; AL024412 antibody; Amp2 antibody; AU014659; antibody; EIF 2 associated p67 homolog antibody; EIF-2-associated p67 antibody; Initiation factor 2 associated 67 kDa glycoprotein antibody; Initiation factor 2-associated 67 kDa glycoprotein antibody; MAP 2 antibody; MAP2 antibody; MAP2_HUMAN antibody; MetAP 2 antibody; Metap2 antibody; Methionine aminopeptidase 2 antibody; Methionyl aminopeptidase 2 antibody; MGC102452 antibody; MGC127390 antibody; MGC53792 antibody; MNPEP antibody; p67 antibody; p67eIF2 antibody; Peptidase M antibody; Peptidase M2 antibody
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宿主：Rabbit
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反應種屬：Human, Rat
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免疫原：Recombinant Human Methionine aminopeptidase 2 protein (313-449AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產品，METAP2 Antibody (CSB-PA013716LA01HU)，的標記方式是Non-conjugated。對于METAP2 Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:20-1:200 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
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基因功能參考文獻：
- MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
- Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
- Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
- Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
- The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
- Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
- This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
- the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
- Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
- the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
- High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
- Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
- MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
- Expressed in colon cancer cells PMID: 15336565
- MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
- A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
- Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
- MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
- Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
- Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176
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亞細胞定位：Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.
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蛋白家族：Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily
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數據庫鏈接：
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