Recombinant Human Methionine aminopeptidase 2 (METAP2)

中文名稱：

人METAP2重組蛋白
貨號：

CSB-YP013716HU
說明書：

MSDS Datasheet
規格：

￥1500
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

METAP2
Uniprot No.：

P50579
別名：

A930035J23Rik; AI047573; AL024412; Amp2; AU014659,; EIF 2 associated p67 homolog; EIF-2-associated p67; Initiation factor 2 associated 67 kDa glycoprotein; Initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MAP2_HUMAN; MetAP 2; Metap2; Methionine aminopeptidase 2; Methionyl aminopeptidase 2; MGC102452; MGC127390; MGC53792; MNPEP; p67; p67eIF2; Peptidase M; Peptidase M2
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

54.8kDa
表達區域：

2-478aa
氨基酸序列

AGVEEVAASGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKKKKKKKRGPKVQTDPPSVPICDLYPNGVFPKGQECEYPPTQDGRTAAWRTTSEEKKALDQASEEIWNDFREAAEAHRQVRKYVMSWIKPGMTMIEICEKLEDCSRKLIKENGLNAGLAFPTGCSLNNCAAHYTPNAGDTTVLQYDDICKIDFGTHISGRIIDCAFTVTFNPKYDTLLKAVKDATNTGIKCAGIDVRLCDVGEAIQEVMESYEVEIDGKTYQVKPIRNLNGHSIGQYRIHAGKTVPIVKGGEATRMEEGEVYAIETFGSTGKGVVHDDMECSHYMKNFDVGHVPIRLPRTKHLLNVINENFGTLAFCRRWLDRLGESKYLMALKNLCDLGIVDPYPPLCDIKGSYTAQFEHTILLRPTCKEVVSRGDDY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
基因功能參考文獻：
1. MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
2. Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
3. Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
4. Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
5. The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
6. Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
7. This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
8. the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
9. Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
10. the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
11. High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
12. Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
13. MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
14. Expressed in colon cancer cells PMID: 15336565
15. MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
16. A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
17. Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
18. MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
19. Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
20. Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176
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亞細胞定位：

Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.
蛋白家族：

Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily
數據庫鏈接：

HGNC: 16672

OMIM: 601870

KEGG: hsa:10988

STRING: 9606.ENSP00000325312

UniGene: Hs.444986