MAP4K2 Antibody

中文名稱：

MAP4K2兔多克隆抗體
貨號：

CSB-PA013437LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA013437LA01HU diluted at 1:500 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA013437LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) MAP4K2 Polyclonal antibody
Uniprot No.：

Q12851
基因名：

MAP4K2
別名：

B lymphocyte serine/threonine protein kinase antibody; B lymphocyte serine/threonine-protein kinase antibody; BL44 antibody; GC kinase antibody; GCK antibody; Germinal center kinase antibody; germinal centre kinase (GC kinase) antibody; M4K2_HUMAN antibody; Map4k2 antibody; MAPK/ERK kinase kinase kinase 2 antibody; MEK kinase kinase 2 antibody; MEKKK 2 antibody; Mitogen activated protein kinase kinase kinase kinase 2 antibody; Mitogen-activated protein kinase kinase kinase kinase 2 antibody; Rab8 interacting protein antibody; Rab8 interacting protein, formerly antibody; Rab8-interacting protein antibody; RAB8IP antibody; RAB8IP, formerly antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Mitogen-activated protein kinase kinase kinase kinase 2 protein (386-469AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，MAP4K2 Antibody (CSB-PA013437LA01HU)，的標記方式是Non-conjugated。對于MAP4K2 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA013437LB01HU	MAP4K2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA013437LC01HU	MAP4K2 Antibody, FITC conjugated
Biotin	CSB-PA013437LD01HU	MAP4K2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion.
基因功能參考文獻：
1. These studies demonstrate, for the first time, that GCK is a molecular therapeutic target in DLBCL tumors and that inhibiting GCK may significantly extend DLBCL patient survival. PMID: 27151888
2. Data indicate 4-substituted 1H-pyrrolo[2,3-b]pyridines as potent inhibitors against TGFbeta-activated kinase 1 (TAK1) and mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2). PMID: 25075558
3. Data suggest that the Rabin8-Rab8-Sec15 interaction may couple the activation of Rab8 to the recruitment of the Rab8 effector and is involved in the regulation of vesicular trafficking for primary cilium formation. PMID: 22433857
4. CCM3 is located on the Golgi apparatus, forming a complex with proteins of the germinal center kinase III (GCKIII) family and GM130, a Golgi-resident protein. PMID: 20332113
亞細胞定位：

Cytoplasm. Basolateral cell membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein.
蛋白家族：

Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
組織特異性：

Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
數據庫鏈接：

HGNC: 6864

OMIM: 603166

KEGG: hsa:5871

STRING: 9606.ENSP00000294066

UniGene: Hs.534341

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