FUNDC1 Antibody
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中文名稱：FUNDC1兔多克隆抗體
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貨號：CSB-PA816886LA01HU
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規(guī)格：￥3900
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圖片：
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Western Blot
Positive WB detected in: Hela whole cell lysate(20μg), MCF-7 whole cell lysate(20μg), A549 whole cell lysate(20μg), PC-3 whole cell lysate(20μg), U-251 whole cell lysate(20μg), K562 whole cell lysate(20μg),Mouse Brain tissue lysate(20μg)
All lanes: FUNDC1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 17-19 kDa
Observed band size: 25 kDa
Exposure time: 300s -
IHC image of CSB-PA816886LA01HU diluted at 1:60 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody -
Immunofluorescence staining of MCF7 cell with CSB-PA816886LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) FUNDC1 Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：FUNDC1; FUN14 domain-containing protein 1
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宿主：Rabbit
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反應(yīng)種屬：Human, Mouse
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免疫原：Recombinant Human FUN14 domain-containing protein 1 protein
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免疫原種屬：Homo sapiens (Human)
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen affinity purification
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Constituents: 50% Glycerol, PBS, pH 7.3 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:20-1:200 IF 1:10-1:100 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
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靶點詳情
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功能：Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.
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基因功能參考文獻：
- Our data suggested that FUNDC1 can be used as a prognostic biomarker in patients with cervical cancer, and may be a new therapeutic target to improve the antitumor effects of chemoradiotherapy. PMID: 28719148
- phosphorylation of Tyr18 of FUNDC1 serves as a molecular switch for mitophagy. PMID: 27653272
- FUNDC1 integrates mitochondrial fission and mitophagy at the interface of the endoplasmic reticulum-mitochondrial contact site by working in concert with DRP1 and calnexin under hypoxic conditions in mammalian cells. PMID: 27145933
- FUNDC1 regulates both mitochondrial fission or fusion and mitophagy. PMID: 27050458
- The BCL2L1-PGAM5-FUNDC1 axis is critical for receptor-mediated mitophagy. PMID: 25126723
- FUNDC1 regulates ULK1 recruitment to damaged mitochondria, where FUNDC1 phosphorylation by ULK1 is crucial for mitophagy. PMID: 24671035
- Data show that Knockdown of endogenous FUNDC1 significantly prevented hypoxia-induced mitophagy, which could be reversed by the expression of wild-type FUNDC1. PMID: 22267086
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亞細(xì)胞定位：Mitochondrion outer membrane; Multi-pass membrane protein.
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蛋白家族：FUN14 family
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組織特異性：Widely expressed.
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數(shù)據(jù)庫鏈接：
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