CHKB Antibody
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中文名稱：CHKB兔多克隆抗體
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貨號：CSB-PA005354LA01HU
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規(guī)格：￥440
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圖片：
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Western Blot
Positive WB detected in: Rat liver tissue, Mouse liver tissue
All lanes: CHKB antibody at 6.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 14 kDa
Observed band size: 46 kDa -
IHC image of CSB-PA005354LA01HU diluted at 1:300 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA005354LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) CHKB Polyclonal antibody
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Uniprot No.：
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基因名：CHKB
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別名：CHETK antibody; Chetk-beta antibody; Chkb antibody; Chkb choline kinase beta antibody; Chkb protein antibody; CHKB_HUMAN antibody; CHKL antibody; Choline kinase beta antibody; Choline kinase-like antibody; Choline kinase-like protein antibody; Choline/ethanolamine kinase antibody; choline/ethanolamine kinase beta antibody; Choline/ethanolamine kinase; beta antibody; CK antibody; CK/EK antibody; CK/EK-beta antibody; CKB antibody; CKEKB antibody; EK antibody; EKB antibody; Ethanolamine kinase antibody; Ethanolamine kinase beta antibody; Ethanolamine kinase; beta antibody; MDCMC antibody
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宿主：Rabbit
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反應(yīng)種屬：Human, Rat, Mouse
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免疫原：Recombinant Human Choline/ethanolamine kinase protein (2-99AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產(chǎn)品，CHKB Antibody (CSB-PA005354LA01HU)，的標記方式是Non-conjugated。對于CHKB Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:100-1:300 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Has a key role in phospholipid metabolism, and catalyzes the first step of phosphatidylethanolamine and phosphatidylcholine biosynthesis.
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基因功能參考文獻：
- findings suggest that CKbeta, in concert with CKalpha, and depending on its phosphorylation status, might play a critical role as a druggable target in carcinogenesis PMID: 27149373
- The whole exome sequencing revealed a novel homozygous missense mutation in the Choline Kinase-Beta (CHKB) gene in exon 9. PMID: 27123443
- A new form of congenital muscular dystrophy with multisystem involvement and characteristic mitochondrial structural changes, due to choline kinase beta gene defects has been characterized. PMID: 26067811
- A novel silent variant in the choline kinase beta causing muscular dystrophy. PMID: 25740612
- Its mutations cause congenital muscular dystrophy.[Review] PMID: 24291895
- study identified a novel haplotype consisting of the indel variation, which had not been detected in previous studies in Japanese and Korean populations, and observed four single-nucleotide polymorphisms in CHKB/CPT1B PMID: 24571861
- CHKB encodes choline kinase beta, an enzyme that catalyzes the first de-novo biosynthetic step of phosphatidylcholine, the most abundant phospholipid in the eukaryotic membrane [review] PMID: 23945283
- CHKB activity was reduced in all three patients, significant reduction in choline:N-acetyl aspartate and choline:creatine ratios in keeping with a general decrease in the amount of choline and phosphocholine-based substrate PMID: 23692895
- The study extends on the observation of a strong multiethnic association of polymorphisms in the TCRA and P2RY11 with narcolepsy, but does not confirm the association of CPT1B/CHKB (rs5770917) in the Chinese population. PMID: 22177342
- homozygous or compound heterozygous mutations in the gene encoding choline kinase beta were identified in individulas with congenital muscular dystrophy. PMID: 21665002
- A single nucleotide polymorphism located between CPT1B and CHKB, was associated with narcolepsy in Japanese (rs5770917[C], odds ratio (OR) = 1.79, combined P = 4.4 x 10(-7)) and other ancestry groups (OR = 1.40, P = 0.02). PMID: 18820697
- Japanese CNS hypersomnias (essential hypersomnia: EHS) other than narcolepsy with cataplexy was significantly associated with SNP rs5770917 (located between CPT1B and CHKB) and HLA-DRB1*1501-DQB1*0602 haplotype PMID: 19404393
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蛋白家族：Choline/ethanolamine kinase family
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