Sheep arachidonic Acid(AA) ELISA kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

綿羊花生四烯酸(AA)酶聯(lián)免疫試劑盒（CSB-EQ027590SH）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的AA含量。試劑盒檢測范圍為15.625 ng/mL-1000 ng/mL，靈敏度為13.797 ng/mL。該產(chǎn)品適用于科研領域中對綿羊模型炎癥機制、營養(yǎng)代謝調(diào)控、藥物干預效果的評價，以及脂質(zhì)代謝相關通路的基礎研究，為體外實驗提供可靠的定量數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

AA
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma
檢測范圍：

15.625 ng/mL-1000 ng/mL
靈敏度：

13.797 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Biochemicals
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	89-100
1:200	Average %	90
	Range %	85-95
1:400	Average %	100
	Range %	94-104
1:800	Average %	105
	Range %	97-110

回收率：

The recovery of sheep AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-106
EDTA plasma (n=4)	102	96-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進展：

AA是一種抗氧化劑，最新研究發(fā)現(xiàn)，AA對于心血管疾病的預防有益。一項針對高危心血管患者的研究表明，AA的補充可以降低冠心病發(fā)生率和死亡率。