Rat thrombin-antithrombin complex,TAT ELISA Kit

產品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

大鼠凝血酶抗凝血酶復合物(TAT)酶聯免疫試劑盒（CSB-E08432r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的TAT含量。大鼠凝血酶抗凝血酶復合物（TAT）是凝血酶與抗凝血酶結合形成的復合物，反映凝血酶生成和活性，用于評估抗凝藥物效果、研究血栓形成機制及血液疾病診斷。通過ELISA技術檢測TAT水平，可監測機體凝血狀態和血栓前狀態。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于科研場景中動態監測凝血激活狀態，如在心血管疾病模型、血栓性疾病動物實驗或藥物干預研究中分析凝血與抗凝血平衡，也可用于體外細胞實驗中探究凝血相關信號通路或藥物對凝血級聯反應的影響。其寬泛的樣本兼容性支持從基礎研究到藥物開發的多層次實驗需求，為凝血功能相關研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

TAT
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	97
	Range %	91-105
1:10	Average %	103
	Range %	97-109
1:20	Average %	95
	Range %	90-98
1:40	Average %	96
	Range %	90-102

回收率：

The recovery of rat TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-94
EDTA plasma (n=4)	94	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.