Mouse thrombin-antithrombin complex,TAT ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠凝血酶抗凝血酶復(fù)合物(TAT)酶聯(lián)免疫試劑盒（CSB-E08433m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TAT含量。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，常用于評(píng)估凝血激活狀態(tài)及相關(guān)病理機(jī)制研究；適用于小鼠模型的血栓性疾病、彌散性血管內(nèi)凝血（DIC）等凝血功能障礙研究，或藥物干預(yù)下凝血/抗凝血系統(tǒng)調(diào)控機(jī)制的體外實(shí)驗(yàn)分析；為心血管疾病、炎癥反應(yīng)或腫瘤相關(guān)凝血異常的動(dòng)物模型研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

TAT
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	87
	Range %	82-93
1:20	Average %	95
	Range %	90-101
1:40	Average %	105
	Range %	96-112
1:80	Average %	93
	Range %	87-98

回收率：

The recovery of mouse TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	93-100
EDTA plasma (n=4)	90	85-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.