Rabbit thrombin-antithrombin complex(TAT)ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔凝血酶抗凝血酶復合物(TAT)酶聯免疫試劑盒（CSB-E17821Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的TAT含量。TAT（轉錄激活因子）是HIV-1病毒的關鍵調控蛋白，其在病毒復制和致病過程中發揮重要作用。TAT通過穩定Tat-TAR復合體增強HIV-1轉錄活性，調控T細胞免疫應答。研究發現，TAT與宿主免疫應答通路相互作用，為HIV治療提供了新的分子靶點。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，該產品適用于科研領域對兔模型凝血功能的研究，如血栓形成機制探索、抗凝藥物療效評估或心血管疾病動物模型中凝血激活程度的動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TAT
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-100
1:2	Average %	92
	Range %	85-98
1:4	Average %	90-105
	Range %	83
1:8	Average %	81-92
	Range %	96

回收率：

The recovery of rabbit TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	84-98
EDTA plasma (n=4)	91	85-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.