Rat oxidized low density lipoprotein,OxLDL ELISA Kit

產(chǎn)品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒（CSB-E07932r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的OxLDL含量。OxLDL即氧化低密度脂蛋白，是體內(nèi)低密度脂蛋白被氧化修飾形成。它在動脈粥樣硬化等疾病發(fā)展中扮演重要角色。研究顯示，OxLDL可被巨噬細胞攝取形成泡沫細胞，還能引發(fā)炎癥反應(yīng)、誘導(dǎo)細胞凋亡等，是心血管疾病研究的重要靶點。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品適用于心血管疾病動物模型研究、藥物干預(yù)效果評估、脂質(zhì)代謝相關(guān)機制探索及細胞氧化應(yīng)激實驗等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

OxLDL
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-96
1:200	Average %	94
	Range %	90-99
1:400	Average %	100
	Range %	96-106
1:800	Average %	90
	Range %	85-96

回收率：

The recovery of rat OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-100
EDTA plasma (n=4)	91	89-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.