Pig oxidized low density lipoprotein,OxLDL ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬氧化低密度脂蛋白(OxLDL)酶聯(lián)免疫試劑盒（CSB-E06527p）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的OxLDL含量。OxLDL（氧化低密度脂蛋白）是一種被氧化修飾的低密度脂蛋白。它與動脈粥樣硬化等疾病密切相關。，OxLDL可被巨噬細胞攝取形成泡沫細胞，促進脂質(zhì)斑塊形成，還能引發(fā)炎癥反應、影響血管內(nèi)皮功能，進而參與疾病的發(fā)生發(fā)展。試劑盒檢測范圍為0.39 nmol/mL-100 nmol/mL，可為心血管疾病機制研究及氧化應激相關模型評估提供關鍵數(shù)據(jù)；適用于基礎科研中探究豬模型動脈粥樣硬化病理進程、評估抗氧化干預效果或代謝性疾病相關研究，為心血管及代謝領域研究者提供穩(wěn)定可靠的氧化應激生物標志物檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

OxLDL
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

0.39 nmol/mL-100 nmol/mL
靈敏度：

0.39 nmol/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-105
1:400	Average %	95
	Range %	83-100
1:800	Average %	95
	Range %	89-100
1:1600	Average %	89
	Range %	85-99

回收率：

The recovery of pig OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	94	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.