Rabbit Interleukin 8,IL-8 ELISA Kit

Rabbit Interleukin 8,IL-8 ELISA Kit

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中文名稱：

兔子白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E06905Rb
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

兔子白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒（CSB-E06905Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CXCL8含量。CXCL8是一種趨化因子，在炎癥反應(yīng)、免疫調(diào)節(jié)等生理病理過(guò)程中發(fā)揮重要作用。其研究機(jī)制主要聚焦于與受體結(jié)合，介導(dǎo)細(xì)胞趨化和激活，可調(diào)節(jié)中性粒細(xì)胞遷移和活化，在感染、腫瘤、自身免疫病等疾病中表達(dá)常異常，是潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為23.5 pg/mL-1500 pg/mL，適用于實(shí)驗(yàn)室中炎癥機(jī)制研究、免疫調(diào)控實(shí)驗(yàn)及藥物開(kāi)發(fā)過(guò)程中的生物標(biāo)志物動(dòng)態(tài)監(jiān)測(cè)，例如在細(xì)菌感染模型、創(chuàng)傷修復(fù)研究或抗炎藥物藥效評(píng)估中量化IL - 8的表達(dá)變化本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant/activation protein 1 ELISA Kit; NAP-1 ELISA Kit; Permeability factor 1 ELISA Kit; PF1 ELISA Kit; rPF1 ELISA Kit
縮寫：

CXCL8
Uniprot No.：

P19874
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	94-105
1:2	Average %	97
	Range %	91-102
1:4	Average %	89
	Range %	85-94
1:8	Average %	90
	Range %	86-98

回收率：

The recovery of rabbit IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-96
EDTA plasma (n=4)	92	89-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.390	2.345	2.368	2.285
750	1.587	1.476	1.532	1.449
375	0.814	0.778	0.796	0.713
187.5	0.409	0.389	0.399	0.316
94	0.276	0.264	0.270	0.187
47	0.182	0.172	0.177	0.094
23.5	0.134	0.131	0.133	0.050
0	0.085	0.081	0.083

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Pathophysiological changes and injury markers for acute lung injury from blunt impact in infant rabbits K Wang,Frontiers in pediatrics,2024
In Vivo Pathogenicity of Methicillin-Susceptible Staphylococcus aureus Strains Carrying Panton–Valentine Leukocidin Gene F Yildirim,Life,2022
Mesenchymal stem cells significantly improved treatment effects of Linezolid on severe pneumonia in a rabbit model Kong D, et al,Bioscience Reports,2019
Cyanidin-3-glucoside attenuates 4-hydroxynonenal and visible light-induced retinal damage in vitro and in vivo Yong Wang, et al,Food & Function,2019
Phenolic-Rich Baccaurea angulata Modulates Inflammatory Biomarkers of Atherosclerosis Muhammad Ibrahim, et al,Journal of Nutrition and Metabolism,2018
Simultaneous Increase in Multiple Proinflammatory Cytokines in the Aqueous Humor in Neovascular Glaucoma With and Without Intravitreal Bevacizumab Injection Ohira S.et al,Investigative ophthalmology & visual science,2015
Assessing the Systemic Toxicity in Rabbits after Sub Acute Exposure to Ocular Irritant Chemicals Reshma CS et al,Toxicol Res,2015
Statins Reduce Ambient Particulate Matter (PM10)-Induced Lung Inflammation by Promoting the Clearance of PM10 from Lung Tissues Miyata R et al,Chest,2013

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平均分:

5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他兔

稀釋比：沒(méi)有稀釋

產(chǎn)品評(píng)價(jià)：我用CSB-E06905Rb檢測(cè)模型組術(shù)前，術(shù)中30min-2h，術(shù)后24h血漿，標(biāo)準(zhǔn)曲線用四參數(shù)模型擬合出來(lái)的R2為0.9998，見(jiàn)圖片，OD值范圍為0.04（Blank)-2.5(1500pg/ml)，標(biāo)準(zhǔn)曲線符合預(yù)期。部分樣本低于標(biāo)曲的Blank吸光度，經(jīng)過(guò)文獻(xiàn)查詢，該模型需要6h才能檢測(cè)出大量IL-8炎癥因子水平，因此屬于正常現(xiàn)象。且得到的數(shù)據(jù)符合預(yù)期趨勢(shì)，后續(xù)會(huì)測(cè)量6h及以上的炎癥因子水平。另回答郵件中的疑問(wèn)：我做的是術(shù)前，術(shù)中0-2h，術(shù)后24h共6個(gè)點(diǎn)的指標(biāo)，沒(méi)做6h的指標(biāo)，因?yàn)?h兔子耳朵采不出動(dòng)脈血了，所以后續(xù)會(huì)在6h處死兔子來(lái)檢測(cè)炎癥指標(biāo)。

By 陳老師

功能：

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
基因功能參考文獻(xiàn)：
1. At the 2-wk time point, interleukin 8 gene expression was comparable in intervertebral discs injected with chondrocytes in controls, whereas its expression in IVDs injected with saline increased 50-fold PMID: 25133623
2. Data show that the serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) decreased significantly in Dachengqi decoction treatment group. PMID: 25270202
3. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways. PMID: 24070709
4. IL-8 and IL-1ra showed correlation with the severity of colitis. These observations should significantly further understandings on the role of neutrophils and monocytes in the immunopathogenesis of ulcerative colitis PMID: 21807533
亞細(xì)胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
數(shù)據(jù)庫(kù)鏈接：

KEGG: ocu:100009129

STRING: 9986.ENSOCUP00000010171

UniGene: Ocu.1938

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.