Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子Ⅰ型膠原(Col Ⅰ)酶聯免疫試劑盒（CSB-E06858Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Col Ⅰ含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本試劑盒通過高特異性抗體對實現靶標捕獲與信號放大，適用于體外研究膠原代謝調控機制、評估纖維化疾病模型進展或探索組織工程材料中膠原沉積規律等科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
縮寫：

Col Ⅰ
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	90
	Range %	85-96
1:4	Average %	88
	Range %	84-92
1:8	Average %	104
	Range %	100-109

回收率：

The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	99	94-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.