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Mouse Collagen Type Ⅰ,Col ⅠELISA Kit

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小鼠Ⅰ型膠原(Col Ⅰ)酶聯免疫試劑盒（CSB-E08083m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的Col Ⅰ含量。Col Ⅰ（Ⅰ型膠原）是細胞外基質主要成分，在維持組織形態和功能上起重要作用。其異常表達與纖維化疾病等密切相關。研究機制多聚焦其合成、降解的調控，信號通路參與情況等，以探索針對 Col Ⅰ的防治策略。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外培養細胞模型、動物組織樣本或體液中的Col Ⅰ蛋白表達研究，可為纖維化疾病機制探索、骨/軟骨再生研究、創傷修復模型構建等科研領域提供可靠數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。

別名：

Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I

縮寫：

Col Ⅰ

種屬：

Mus musculus (Mouse)

樣本類型：

serum, plasma, cell culture supernates, tissue homogenates

檢測范圍：

0.156 ng/mL-10 ng/mL

靈敏度：

0.039 ng/mL

反應時間：

1-5h

樣本體積：

50-100ul

檢測波長：

450 nm

研究領域：

Signal Transduction

測定原理：

quantitative

測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ColⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-97
1:200	Average %	96
	Range %	91-104
1:400	Average %	97
	Range %	94-101
1:800	Average %	98
	Range %	89-102

回收率：

The recovery of mouse ColⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	98-107
EDTA plasma (n=4)	92	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.595	2.494	2.545	2.355
5	1.904	1.803	1.854	1.664
2.5	1.148	1.128	1.138	0.948
1.25	0.660	0.671	0.666	0.476
0.625	0.432	0.442	0.437	0.247
0.312	0.304	0.315	0.310	0.120
0.156	0.249	0.258	0.254	0.064
0	0.189	0.190	0.190

本試劑盒所含材料：

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse Col I antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled Col I antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

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本試劑盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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數據處理：

ELISA操作視頻及標曲繪制軟件下載

貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.