Pig Lysyl Oxidase ELISA Kit

說明書下載

中文名稱：

豬賴氨酰氧化酶酶聯(lián)免疫試劑盒
貨號：

CSB-E15813p
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豬賴氨酰氧化酶酶聯(lián)免疫試劑盒（CSB-E15813p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LOX含量。LOX（脂氧化酶）是一種催化花生四烯酸生成生物活性分子的關(guān)鍵酶，廣泛分布于人體，參與細胞信號傳導和代謝。研究發(fā)現(xiàn)，LOX在多種惡性腫瘤中表達增高，其代謝產(chǎn)物與腫瘤的發(fā)生、發(fā)展密切相關(guān)。研究LOX的分子生物學特征和促癌機制，有助于開發(fā)新的抗腫瘤藥物。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，可廣泛應用于豬模型的組織病理研究，例如評估纖維化疾病進程中LOX的表達動態(tài)、腫瘤轉(zhuǎn)移相關(guān)基質(zhì)硬化研究，或心血管疾病模型中膠原沉積的分子機制解析，為畜牧疾病模型構(gòu)建和基礎醫(yī)學研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

LOX ELISA Kit; Protein-lysine 6-oxidase ELISA Kit; EC 1.4.3.13 ELISA Kit; Lysyl oxidase) [Cleaved into: Protein-lysine 6-oxidase ELISA Kit; long form; Protein-lysine 6-oxidase ELISA Kit; short form] ELISA Kit
縮寫：

LOX
Uniprot No.：

P45845
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig lysyl oxidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-89
1:2	Average %	93
	Range %	90-95
1:4	Average %	106
	Range %	101-111
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of pig lysyl oxidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-85
EDTA plasma (n=4)	106	103-109

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.073	2.000	2.037	1.950
50	1.616	1.636	1.626	1.539
25	1.149	1.201	1.175	1.088
12.5	0.721	0.735	0.728	0.641
6.25	0.440	0.457	0.449	0.362
3.12	0.286	0.269	0.278	0.191
1.56	0.198	0.187	0.193	0.106
0	0.085	0.089	0.087

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture.
亞細胞定位：

Secreted. Secreted, extracellular space.
蛋白家族：

Lysyl oxidase family
數(shù)據(jù)庫鏈接：

STRING: 9823.ENSSSCP00000015138

UniGene: Ssc.10386

Pig Lysyl Oxidase ELISA Kit

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