Mouse Protein disulfide-isomerase(P4HB) ELISA kit

說明書下載

中文名稱：

小鼠蛋白質(zhì)二硫鍵異構(gòu)酶(P4HB)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL017342MO
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠蛋白質(zhì)二硫鍵異構(gòu)酶(P4HB)酶聯(lián)免疫試劑盒（CSB-EL017342MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的P4HB含量。P4HB 即脯氨酰 4-羥化酶β亞基，是內(nèi)質(zhì)網(wǎng)內(nèi)的多功能蛋白。其參與膠原蛋白合成，對(duì)維持細(xì)胞外基質(zhì)穩(wěn)定至關(guān)重要。在研究機(jī)制上，它作為伴侶蛋白促進(jìn)蛋白質(zhì)折疊，還參與氧化還原反應(yīng)。P4HB 與多種疾病相關(guān)，是潛在的治療靶點(diǎn)。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，為研究蛋白質(zhì)折疊調(diào)控機(jī)制、氧化應(yīng)激相關(guān)疾病模型（如肝臟疾病或代謝異常）以及內(nèi)質(zhì)網(wǎng)功能相關(guān)分子通路提供可靠工具；適用于體外實(shí)驗(yàn)中探索P4HB在細(xì)胞生理病理過程中的表達(dá)變化及其分子互作研究，滿足基礎(chǔ)科研中對(duì)目標(biāo)蛋白定量分析的需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

P4hb ELISA Kit; Pdia1Protein disulfide-isomerase ELISA Kit; PDI ELISA Kit; EC 5.3.4.1 ELISA Kit; Cellular thyroid hormone-binding protein ELISA Kit; Endoplasmic reticulum resident protein 59 ELISA Kit; ER protein 59 ELISA Kit; ERp59 ELISA Kit; Prolyl 4-hydroxylase subunit beta ELISA Kit; p55 ELISA Kit
縮寫：

P4HB
Uniprot No.：

P09103
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse P4HB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-98
1:2	Average %	103
	Range %	96-108
1:4	Average %	92
	Range %	85-97
1:8	Average %	101
	Range %	95-107

回收率：

The recovery of mouse P4HB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-95
EDTA plasma (n=4)	99	91-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.248	2.175	2.212	2.044
10	1.615	1.584	1.600	1.432
5	1.016	0.967	0.992	0.824
2.5	0.545	0.536	0.541	0.373
1.25	0.342	0.358	0.350	0.182
0.625	0.251	0.243	0.247	0.079
0.312	0.215	0.210	0.213	0.045
0	0.171	0.165	0.168

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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功能：

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
基因功能參考文獻(xiàn)：
1. Unfolded protein response caused by protein misfolding, may lead to PDI-dependent NOX activation and contribute to neurotoxicity in neurodegenerative diseases including ALS. PMID: 23118353
2. PDI from endothelial cells is required for fibrin generation in vivo. PMID: 20668226
3. Protein disulfide isomerase (PDI) on the platelet surface is recognized by anti-dengue virus NS1 antibodies. PMID: 19822367
亞細(xì)胞定位：

Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein.
蛋白家族：

Protein disulfide isomerase family
數(shù)據(jù)庫鏈接：

KEGG: mmu:18453

STRING: 10090.ENSMUSP00000026122

UniGene: Mm.16660

Mouse Protein disulfide-isomerase(P4HB) ELISA kit

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