Mouse Mannan-binding lectin serine protease 2(MASP2) ELISA kit

文獻引用 (1) 說明書下載

中文名稱：

小鼠甘露糖結合凝集素相關絲氨酸蛋白酶2(MASP2)酶聯免疫試劑盒
貨號：

CSB-EL013510MO
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠甘露糖結合凝集素相關絲氨酸蛋白酶2(MASP2)酶聯免疫試劑盒（CSB-EL013510MO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MASP2含量。MASP2是一種血清蛋白酶，通過甘露糖結合凝集素激活補體系統，裂解C2和C4，形成C3轉化酶，是補體級聯中的關鍵步驟。其研究機制涉及MASP2的激活、裂解和補體系統的激活過程。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于免疫學、炎癥機制、補體系統功能等領域的基礎研究，如探索感染性疾病模型、自身免疫疾病動物實驗中MASP2的動態變化及其調控機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Masp2Mannan-binding lectin serine protease 2 ELISA Kit; EC 3.4.21.104 ELISA Kit; MBL-associated serine protease 2 ELISA Kit; Mannose-binding protein-associated serine protease 2 ELISA Kit; MASP-2) [Cleaved into: Mannan-binding lectin serine protease 2 A chain; Mannan-binding lectin serine protease 2 B chain] ELISA Kit
縮寫：

MASP2
Uniprot No.：

Q91WP0
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

125 pg/mL-8000 pg/mL
靈敏度：

31.2 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MASP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
Range %	89-95
1:200	Average %	101
Range %	97-106
1:400	Average %	86
Range %	83-90
1:800	Average %	102
Range %	97-107

回收率：

The recovery of mouse MASP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	100	95-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
8000	0.100	0.106	0.103
4000	0.243	0.240	0.242
2000	0.358	0.361	0.360
1000	0.516	0.531	0.524
500	0.701	0.740	0.721
250	1.027	1.100	1.064
125	1.507	1.496	1.502
0	2.311	2.298	2.305

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Mannose-binding lectin-associated serine protease 2 (MASP-2) contributes to poor disease outcome in humans and mice with pneumococcal meningitis E. Soemirien Kasanmoentalib.et al,Journal of Neuroinflammati ,2017

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功能：

Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.
基因功能參考文獻：
1. MASP-2 has an essential role in the mediation of cerebral ischemia-reperfusion injury. PMID: 27577570
2. this study shows that MASP-2 contributes to the development of collagen Ab-induced arthritis PMID: 28739878
3. We induced anti-myeloperoxidase vasculitis in mice and showed that neither MASP-2- nor properdin-deficient mice were protected, suggesting that alternative pathway activation does not require properdin or the lectin pathway. PMID: 27235854
4. Mannan-binding lectin-associated serine protease 2 is critical for the development of renal ischemia reperfusion injury and mediates tissue injury in the absence of complement C4. PMID: 24868011
5. Gene targeting of mouse TDP-43 negatively affects Masp2 expression. PMID: 24740308
6. ALI in H5N1-infected mice was caused by excessive complement activation, as demonstrated by deposition of C3, C5b-9, and MBL-C in lung tissue, and by up-regulation of MBL-associated serine protease-2 and the complement receptors C3aR and C5aR. PMID: 23526211
7. study corroborates the essential function of MASP-2 in the lectin pathway and highlights the importance of mannan-binding lectin-independent lectin pathway activation in the host defense against pneumococci PMID: 22792067
8. Targeting of mannan-binding lectin-associated serine protease-2 confers protection from myocardial and gastrointestinal ischemia/reperfusion injury. PMID: 21502512
9. Highly conserved intron sequences. Restricted expression contrasts with the ubiquitous expression of neighboring genes. PMID: 15672593
10. Recombinant protein of masp2 was made and purified. PMID: 15826378
11. MASP-2 is essential for the activation of C4 and sMAP plays a regulatory role in the activation of the lectin pathway. PMID: 17142762
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亞細胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
組織特異性：

Plasma.
數據庫鏈接：

KEGG: mmu:17175

STRING: 10090.ENSMUSP00000049729

UniGene: Mm.378962

Mouse Mannan-binding lectin serine protease 2(MASP2) ELISA kit

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