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中文名稱：小鼠肌酸激酶同工酶MB(CK-MB)酶聯免疫試劑盒
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貨號：CSB-E14404m
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規格：96T/48T
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價格：￥3600/￥2500
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其他：
產品詳情
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產品描述：小鼠肌酸激酶同工酶MB(CK-MB)酶聯免疫試劑盒（CSB-E14404m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CK-MB含量。CK-MB即肌酸激酶同工酶，是心肌損傷的重要標志物。其釋放量與心肌損傷程度相關。在研究機制上，主要通過檢測血液中CK-MB水平變化，輔助診斷急性心肌梗死等心臟疾病，評估病情及預后。試劑盒檢測范圍為1.25 mU/mL-80 mU/mL，適用于心血管疾病機制研究、心肌缺血再灌注損傷模型評估、藥物心臟毒性篩選等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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縮寫：CK-MB
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍：1.25 mU/mL-80 mU/mL
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靈敏度：0.312 mU/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Cardiovascular
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 80-99 1:2 Average % 95 Range % 86-105 1:4 Average % 91 Range % 82-100 1:8 Average % 93 Range % 82-101 -
回收率：
The recovery of mouse CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 98 94-105 EDTA plasma (n=4) 95 89-98 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mU/ml OD1 OD2 Average Corrected 80 2.912 2.787 2.850 2.684 40 2.386 2.374 2.380 2.214 20 1.617 1.664 1.641 1.475 10 0.846 0.895 0.871 0.705 5 0.553 0.523 0.538 0.372 2.5 0.318 0.336 0.327 0.161 1.25 0.246 0.252 0.249 0.083 0 0.164 0.168 0.166 -
數據處理：
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貨期：3-5 working days
引用文獻
- PPARA ameliorates sepsis-induced myocardial injury via promoting macrophage M2 polarization by interacting with DUSP1 L Cheng,Regenerative therapy,2024
- Golden bifid treatment regulates gut microbiota and serum metabolites to improve myocardial dysfunction in cecal ligation and puncture-induced sepsis mice M Wang,Biochimica et biophysica acta. Molecular basis of disease,2024
- Inhibition of miR-143-3p alleviates myocardial ischemia reperfusion injury via limiting mitochondria-mediated apoptosis CH Lu,Biological chemistry,2023
- Dihydrotanshinone I preconditions myocardium against ischemic injury via PKM2 glutathionylation sensitive to ROS X Wu,Acta Pharmaceutica Sinica B,2022
- Propionate alleviates myocardial ischemia-reperfusion injury aggravated by Angiotensin II dependent on caveolin-1/ACE2 axis through GPR41 Fan Deng,International journal of biological sciences,2022
- Propionate alleviates myocardial ischemia-reperfusion injury aggravated by Angiotensin II dependent on caveolin-1/ACE2 axis through GPR41 F Deng,International Journal of Biological Sciences,2021
- Long non-coding RNA THRIL is upregulated in coronary heart disease and binds to microRNA-424 to upregulate TXNIP in mice L Lin,Microvascular Research,2021
- Cardiomyocyte-specific deletion of Senp2 contributes to CVB3 viral replication and inflammation K Wang,International Immunopharmacology,2020
- Follistatin-Like 1 Protects against Doxorubicin-Induced Cardiomyopathy through Upregulation of Nrf2 Y Zhao,Oxidative Medicine and Cellular Longevity,2020
- Tanshinone IIA attenuates cardiac microvascular ischemia-reperfusion injury via regulating the SIRT1-PGC1α-mitochondrial apoptosis pathway Zhong J, et al,Cell Stress and Chaperones,2019
- Fine particulate matter-induced cardiovascular injury is associated with NLRP3 inflammasome activation in Apo E-/- mice Du X, et al,Ecotoxicology and environmental safety,2019
- Ulinastatin Protects against CVB3-Induced Acute Viral Myocarditis through Nrf2 Activation Song F.et al,Inflammation,2018
- A water-soluble nucleolin aptamer-paclitaxel conjugate for tumor-specific targeting in ovarian cancer Li F.et al,Nat Commun.,2017
產品評價
平均分:
樣品類型：血清
樣品信息：小鼠
稀釋比：沒有稀釋
產品評價： 我用CSB-E14404m檢測小鼠血清中CK-MB含量，未進行稀釋，結果在標曲范圍內，靈敏度優異，標曲的OD值分別為0.117，0.13，0.148，0.185，0.222，0.619，1.472，2.755
By 沈老師
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